Now showing items 1-20 of 25

    • Biochemical and Crystallographic Studies of the Escherichia Coli β-Barrel Assembly Machine 

      Westwood, David (2016-09-01)
      Bacteria have developed resistance mechanisms to every class of antibiotics that has been created and combating the rise of multidrug-resistant bacteria—a major threat to public health—requires the continual development ...
    • Centromere-specific histone Cse4 by the chaperone Scm3 

      Cho, Uhn-Soo; Harrison, Stephen C. (BioMed Central, 2013)
    • Crystal Structure of the Yeast Inner Kinetochore Subunit cep3p 

      Bellizzi, John J. III; Sorger, Peter; Harrison, Stephen (Elsevier, 2007)
      In budding yeast, the four-protein CBF3 complex (Skp1p-Ctf13p-Cep3p-Ndc10p) initiates kinetochore assembly by binding to the CDEIII locus of centromeric DNA. A Cep3p dimer recruits a Ctf13p-Skp1p heterodimer and contacts ...
    • Data publication with the structural biology data grid supports live analysis 

      Meyer, Peter A.; Socias, Stephanie; Key, Jason; Ransey, Elizabeth; Tjon, Emily C.; Buschiazzo, Alejandro; Lei, Ming; Botka, Chris; Withrow, James; Neau, David; Rajashankar, Kanagalaghatta; Anderson, Karen S.; Baxter, Richard H.; Blacklow, Stephen C.; Boggon, Titus J.; Bonvin, Alexandre M. J. J.; Borek, Dominika; Brett, Tom J.; Caflisch, Amedeo; Chang, Chung-I; Chazin, Walter J.; Corbett, Kevin D.; Cosgrove, Michael S.; Crosson, Sean; Dhe-Paganon, Sirano; Di Cera, Enrico; Drennan, Catherine L.; Eck, Michael J.; Eichman, Brandt F.; Fan, Qing R.; Ferré-D'Amaré, Adrian R.; Christopher Fromme, J.; Garcia, K. Christopher; Gaudet, Rachelle; Gong, Peng; Harrison, Stephen C.; Heldwein, Ekaterina E.; Jia, Zongchao; Keenan, Robert J.; Kruse, Andrew C.; Kvansakul, Marc; McLellan, Jason S.; Modis, Yorgo; Nam, Yunsun; Otwinowski, Zbyszek; Pai, Emil F.; Pereira, Pedro José Barbosa; Petosa, Carlo; Raman, C. S.; Rapoport, Tom A.; Roll-Mecak, Antonina; Rosen, Michael K.; Rudenko, Gabby; Schlessinger, Joseph; Schwartz, Thomas U.; Shamoo, Yousif; Sondermann, Holger; Tao, Yizhi J.; Tolia, Niraj H.; Tsodikov, Oleg V.; Westover, Kenneth D.; Wu, Hao; Foster, Ian; Fraser, James S.; Maia, Filipe R. N C.; Gonen, Tamir; Kirchhausen, Tom; Diederichs, Kay; Crosas, Mercè; Sliz, Piotr (Nature Publishing Group, 2016)
      Access to experimental X-ray diffraction image data is fundamental for validation and reproduction of macromolecular models and indispensable for development of structural biology processing methods. Here, we established ...
    • Distinct functional determinants of influenza hemagglutinin-mediated membrane fusion 

      Ivanovic, Tijana; Harrison, Stephen C (eLife Sciences Publications, Ltd, 2015)
      Membrane fusion is the critical step for infectious cell penetration by enveloped viruses. We have previously used single-virion measurements of fusion kinetics to study the molecular mechanism of influenza-virus envelope ...
    • Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant 

      Earnshaw, W. C.; Allshire, R. C.; Black, B. E.; Bloom, K.; Brinkley, B. R.; Brown, W.; Cheeseman, I. M.; Choo, K. H. A.; Copenhaver, G. P.; DeLuca, J. G.; Desai, A.; Diekmann, S.; Erhardt, S.; Fitzgerald-Hayes, M.; Foltz, D.; Fukagawa, T.; Gassmann, R.; Gerlich, D. W.; Glover, D. M.; Gorbsky, G. J.; Harrison, Stephen C.; Heun, P.; Hirota, T.; Jansen, L. E. T.; Karpen, G.; Kops, G. J. P. L.; Lampson, M. A.; Lens, S. M.; Losada, A.; Luger, K.; Maiato, H.; Maddox, P. S.; Margolis, R. L.; Masumoto, H.; McAinsh, A. D.; Mellone, B. G.; Meraldi, P.; Musacchio, A.; Oegema, K.; O’Neill, R. J.; Salmon, E. D.; Scott, K. C.; Straight, A. F.; Stukenberg, P. T.; Sullivan, B. A.; Sullivan, K. F.; Sunkel, C. E.; Swedlow, J. R.; Walczak, C. E.; Warburton, P. E.; Westermann, S.; Willard, H. F.; Wordeman, L.; Yanagida, M.; Yen, T. J.; Yoda, K.; Cleveland, D. W. (Springer Netherlands, 2013)
      The first centromeric protein identified in any species was CENP-A, a divergent member of the histone H3 family that was recognised by autoantibodies from patients with scleroderma-spectrum disease. It has recently been ...
    • Influenza-virus membrane fusion by cooperative fold-back of stochastically induced hemagglutinin intermediates 

      Ivanovic, Tijana; Choi, Jason L; Whelan, Sean P; van Oijen, Antoine M; Harrison, Stephen C (eLife Sciences Publications, Ltd, 2013)
      Influenza virus penetrates cells by fusion of viral and endosomal membranes catalyzed by the viral hemagglutinin (HA). Structures of the initial and final states of the HA trimer define the fusion endpoints, but do not ...
    • Interactions of Lipids with Aquaporin-0 and Other Membrane Proteins 

      Hite, Richard; Tamir, Gonen; Harrison, Stephen; Walz, Thomas (Springer Verlag, 1995)
      The structure of aquaporin-0 (AQP0) has recently been determined by electron crystallography of two-dimensional (2D) crystals and by x-ray crystallography of three-dimensional (3D) crystals. The electron crystallographic ...
    • Key mutations stabilize antigen-binding conformation during affinity maturation of a broadly neutralizing influenza antibody lineage 

      Xu, Huafeng; Schmidt, Aaron G; O'Donnell, Timothy; Therkelsen, Matthew D; Kepler, Thomas B; Moody, M Anthony; Haynes, Barton F; Liao, Hua-Xin; Harrison, Stephen C; Shaw, David E (BlackWell Publishing Ltd, 2014)
      Affinity maturation, the process in which somatic hypermutation and positive selection generate antibodies with increasing affinity for an antigen, is pivotal in acquired humoral immunity. We have studied the mechanism of ...
    • Kinetics of Proton Transport into Influenza Virions by the Viral M2 Channel 

      Ivanovic, Tijana; Rozendaal, Rutger; Floyd, Daniel Lee; Popovic, Milos; van Oijen, Antoine M.; Harrison, Stephen C. (Public Library of Science, 2012)
      M2 protein of influenza A viruses is a tetrameric transmembrane proton channel, which has essential functions both early and late in the virus infectious cycle. Previous studies of proton transport by M2 have been limited ...
    • Lipid-Protein Interactions in Double-Layered Two-Dimensional AQP0 Crystals 

      Gonen, Tamir; Cheng, Yifan; Sliz, Piotr; Hiroaki, Yoko; Fujiyoshi, Yoshinori; Harrison, Stephen; Walz, Thomas (Nature Publishing Group, 2005)
      Lens-specific aquaporin-0 (AQP0) functions as a specific water pore and forms the thin junctions between fibre cells. We describe a 1.9 Å resolution structure of junctional AQP0, determined by electron crystallography of ...
    • Mechanism for Coordinated RNA Packaging and Genome Replication by Rotavirus Polymerase VP1 

      Lu, Xiaohui; McDonald, Sarah M.; Tortorici, M. Alejandra; Tao, Yizhi Jane; Carpio, Rodrigo Vasquez-Del; Nibert, Max; Patton, John T.; Harrison, Stephen (Elsevier (Cell Press), 2008)
      Rotavirus RNA-dependent RNA polymerase, VP1, catalyzes RNA synthesis within a subviral particle. This activity depends on core shell protein VP2. A conserved sequence at the 3′ end of plusstrand RNA templates is important ...
    • Mechanisms of Membrane Disruption by Viral Entry Proteins 

      Kim, Irene (2013-02-15)
      To enter and infect cells, viruses must overcome the barrier presented by the cell membrane. Enveloped viruses, which possess their own lipid bilayer, fuse their viral membrane with the cell membrane. Non-enveloped viruses, ...
    • A Molecular Basis for the Coupling of Sister Centromeres 

      Hinshaw, Stephen M. (2016-08-18)
      Chromosome segregation during mitosis requires that two forces act on DNA: one that pulls chromatids away from each other and towards presumptive daughter cells, and another that holds chromatids together and resists the ...
    • Peptide Inhibitors of Dengue-Virus Entry Target a Late-Stage Fusion Intermediate 

      Schmidt, Aaron Gregory; Yang, Priscilla; Harrison, Stephen C. (Public Library of Science, 2010)
      The mechanism of membrane fusion by “class II” viral fusion proteins follows a pathway that involves large-scale domain rearrangements of the envelope glycoprotein (E) and a transition from dimers to trimers. The rearrangement ...
    • Phosphorylated T Cell Receptor ζ-Chain and ZAP70 Tandem SH2 Domains Form a 1:3 Complex In Vitro 

      Weissenhorn, Winfried; Eck, Michael; Harrison, Stephen C.; Wiley, Don C. (Blackwell Science on behalf of the Federation of European Biochemical Societies, 1996)
      The ζ polypeptide is part of the T cell antigen receptor (TCR). The ζ-chain contributes to efficient cell-surface expression of the TCR and accounts for part of its signal transduction capability. TCR recognition triggers ...
    • Professional Values and Reported Behaviours of Doctors in the USA and UK: Quantitative Survey 

      Roland, Martin; Rao, Sowmya R.; Sibbald, Bonnie; Hann, Mark; Harrison, Stephen C.; Walter, Alex; Guthrie, Bruce; Desroches, Catherine; Ferris, Timothy Gregg; Campbell, Eric G. (BMJ Group, 2011)
      Background: The authors aimed to determine US and UK doctors’ professional values and reported behaviours, and the extent to which these vary with the context of care. Method: 1891 US and 1078 UK doctors completed the ...
    • Protein Arms in the Kinetochore-Microtubule Interface of the Yeast DASH Complex 

      Miranda, JJ L.; King, David S.; Harrison, Stephen (American Society for Cell Biology, 2007)
      The yeast DASH complex is a heterodecameric component of the kinetochore necessary for accurate chromosome segregation. DASH forms closed rings around microtubules with a large gap between the DASH ring and the microtubule ...
    • Sequential conformational rearrangements in flavivirus membrane fusion 

      Chao, Luke H; Klein, Daryl E; Schmidt, Aaron G; Peña, Jennifer M; Harrison, Stephen C (eLife Sciences Publications, Ltd, 2014)
      The West Nile Virus (WNV) envelope protein, E, promotes membrane fusion during viral cell entry by undergoing a low-pH triggered conformational reorganization. We have examined the mechanism of WNV fusion and sought evidence ...
    • Small-Molecule Inhibitors of Dengue-Virus Entry 

      Schmidt, Aaron Gregory; Lee, Kyungae; Yang, Priscilla; Harrison, Stephen C. (Public Library of Science, 2012)
      Flavivirus envelope protein (E) mediates membrane fusion and viral entry from endosomes. A low-pH induced, dimer-to-trimer rearrangement and reconfiguration of the membrane-proximal "stem" of the E ectodomain draw together ...