Now showing items 1-20 of 31

    • Angiotensin II Induced Cardiac Dysfunction on a Chip 

      Horton, Renita E.; Yadid, Moran; McCain, Megan L.; Sheehy, Sean P.; Pasqualini, Francesco S.; Park, Sung-Jin; Cho, Alexander; Campbell, Patrick; Parker, Kevin Kit (Public Library of Science, 2016)
      In vitro disease models offer the ability to study specific systemic features in isolation to better understand underlying mechanisms that lead to dysfunction. Here, we present a cardiac dysfunction model using angiotensin ...
    • Blast-induced phenotypic switching in cerebral vasospasm 

      Alford, Patrick W.; Dabiri, Borna Esfahani; Goss, Josue; Hemphill, Matthew Allen; Brigham, Mark Daniel; Parker, Kevin Kit (Proceedings of the National Academy of Sciences, 2011)
      Vasospasm of the cerebrovasculature is a common manifestation of blast-induced traumatic brain injury (bTBI) reported among combat casualties in the conflicts in Afghanistan and Iraq. Cerebral vasospasm occurs more frequently, ...
    • Cell-to-cell coupling in engineered pairs of rat ventricular cardiomyocytes: relation between Cx43 immunofluorescence and intercellular electrical conductance 

      McCain, Megan; Desplantez, Thomas; Geisse, Nicholas A.; Rothen-Rutishauser, Barbara; Oberer, Helene; Parker, Kevin Kit; Kleber, Andre (American Physiological Society, 2012)
      Gap junctions are composed of connexin (Cx) proteins, which mediate intercellular communication. Cx43 is the dominant Cx in ventricular myocardium, and Cx45 is present in trace amounts. Cx43 immunosignal has been associated ...
    • Coupling primary and stem cell–derived cardiomyocytes in an in vitro model of cardiac cell therapy 

      Aratyn-Schaus, Yvonne; Pasqualini, Francesco S.; Yuan, Hongyan; McCain, Megan L.; Ye, George J.C.; Sheehy, Sean P.; Campbell, Patrick H.; Parker, Kevin Kit (The Rockefeller University Press, 2016)
      The efficacy of cardiac cell therapy depends on the integration of existing and newly formed cardiomyocytes. Here, we developed a minimal in vitro model of this interface by engineering two cell microtissues (μtissues) ...
    • Cyclic Strain Induces Dual-Mode Endothelial-Mesenchymal Transformation of the Cardiac Valve 

      Balachandran, Kartik; Alford, Patrick W.; Wylie-Sears, Jill; Goss, Josue; Grosberg, Anna; Bischoff, Joyce E.; Aikawa, Elena; Levine, Robert A.; Parker, Kevin Kit (Proceedings of the National Academy of Sciences, 2011)
      Endothelial-mesenchymal transformation (EMT) is a critical event for the embryonic morphogenesis of cardiac valves. Inducers of EMT during valvulogenesis include VEGF, TGF-β1, and wnt/β-catenin (where wnt refers to the ...
    • Design and Assembly Considerations in the Engineering of Vascular Tissue 

      Ye, Jin Cheng (2014-02-25)
      Native vascular tissue functions are highly dependent on structural organization at the super-cellular, cellular, and sub-cellular spatial scales. We hypothesized that the structure-function relationship of vascular tissues ...
    • Design Considerations for Engineered Myocardium 

      Sheehy, Sean Paul (2014-06-06)
      The fabrication of biomimetic heart muscle suitable for pharmaceutical compound evaluation and disease modeling is hindered by limitations in our understanding of how to guide and assess the maturity of engineered myocardium ...
    • Differential Contributions of Conformation Extension and Domain Unfolding to Properties of Fibronectin Nanotextiles 

      Deravi, Leila F; Su, Tianxiang; Paten, Jeffrey A.; Ruberti, Jeffrey W.; Bertoldi, Katia; Parker, Kevin Kit (American Chemical Society, 2012)
      Fibronectin (FN) textiles are built as nanometer-thick fabrics. When uniaxially loaded, these fabrics exhibit a distinct threshold between elastic and plastic deformation with increasing stretch. Fabric mechanics are ...
    • Ensembles of engineered cardiac tissues for physiological and pharmacological study: Heart on a chip 

      Grosberg, Anna; Alford, Patrick W.; McCain, Megan L.; Parker, Kevin Kit (Royal Society of Chemistry (RSC), 2011)
      Traditionally, muscle physiology experiments require multiple tissue samples to obtain morphometric, electrophysiological, and contractility data. Furthermore, these experiments are commonly completed one at a time on cover ...
    • From Womb to Doom: Mechanical Regulation of Cardiac Tissue Assembly in Morphogenesis and Pathogenesis 

      McCain, Megan Laura (2013-02-14)
      The assembly, form, and function of the heart is regulated by complex mechanical signals originating from intrinsic and extrinsic sources, such as the cytoskeleton and the extracellular matrix. During development, mechanical ...
    • Hierarchical architecture influences calcium dynamics in engineered cardiac muscle 

      Pong, T.; Adams, William James; Bray, Mark-Anthony; Feinberg, Adam W.; Sheehy, Sean Paul; Werdich, Andreas A.; Parker, Kevin Kit (SAGE Publications, 2011)
      Changes in myocyte cell shape and tissue structure are concurrent with changes in electromechanical function in both the developing and diseased heart. While the anisotropic architecture of cardiac tissue is known to ...
    • Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing 

      Lind, Johan Ulrik; Busbee, Travis Alexander; Valentine, Alexander; Pasqualini, Francesco; Yuan, Hongyan; Yedid, Moran; Park, Sung-Jin; Kotikian, Arda; Nesmith, Alexander Peyton; Campbell, Patrick H.; Vlassak, Joost J.; Lewis, Jennifer; Parker, Kevin Kit (Springer Nature, 2016)
      Biomedical research has relied on animal studies and conventional cell cultures for decades. Recently, microphysiological systems (MPS), also known as organs-on-chips, that recapitulate the structure and function of native ...
    • Inter- and Intracellular Effects of Traumatic Axonal Injury 

      Dabiri, Borna Esfahani (2014-06-06)
      Mild Traumatic Brain Injuries (mTBIs) are non-penetrating brain injuries that do not result in gross pathological lesions, yet they may cause a spectrum of cognitive and behavioral deficits. mTBI has been placed in the ...
    • Mechanotransduction and Metabolism in Cardiomyocyte Microdomains 

      Pasqualini, Francesco S.; Nesmith, Alexander P.; Horton, Renita E.; Sheehy, Sean P.; Parker, Kevin Kit (Hindawi Publishing Corporation, 2016)
      Efficient contractions of the left ventricle are ensured by the continuous transfer of adenosine triphosphate (ATP) from energy production sites, the mitochondria, to energy utilization sites, such as ionic pumps and the ...
    • Mechanotransduction: the role of mechanical stress, myocyte shape, and cytoskeletal architecture on cardiac function 

      McCain, Megan L.; Parker, Kevin Kit (Springer Nature, 2011)
      Mechanotransduction refers to the conversion of mechanical forces into biochemical or electrical signals that initiate structural and functional remodeling in cells and tissues. The heart is a kinetic organ whose form ...
    • Metrics for Assessing Cytoskeletal Orientational Correlations and Consistency 

      Drew, Nancy K.; Eagleson, Mackenzie A.; Baldo Jr., Danny B.; Parker, Kevin Kit; Grosberg, Anna (Public Library of Science, 2015)
      In biology, organization at multiple scales potentiates biological function. Current advances in staining and imaging of biological tissues provide a wealth of data, but there are few metrics to quantitatively describe ...
    • Microfluidic heart on a chip for higher throughput pharmacological studies 

      Agarwal, Ashutosh; Goss, Josue Adrian; Cho, Alexander; McCain, Megan Laura; Parker, Kevin Kit (Royal Society of Chemistry (RSC), 2013)
      We present the design of a higher throughput “heart on a chip” which utilizes a semi-automated fabrication technique to process sub millimeter sized thin film cantilevers of soft elastomers. Anisotropic cardiac microtissues ...
    • Micropatterning Alginate Substrates for In Vitro Cardiovascular Muscle on a Chip 

      Agarwal, Ashutosh; Farouz, Yohan; Nesmith, Alexander Peyton; Deravi, Leila F.; McCain, Megan Laura; Parker, Kevin Kit (Wiley-Blackwell, 2013)
      Soft hydrogels such as alginate are ideal substrates for building muscle in vitro because they have structural and mechanical properties close to the in vivo extracellular matrix (ECM) network. However, hydrogels are ...
    • Modeling of cardiac muscle thin films: Pre-stretch, passive and active behavior 

      Shim, Jongmin; Grosberg, Anna; Nawroth, Janna; Parker, Kevin Kit; Bertoldi, Katia (Elsevier, 2012)
      Recent progress in tissue engineering has made it possible to build contractile bio-hybrid materials that undergo conformational changes by growing a layer of cardiac muscle on elastic polymeric membranes. Further development ...
    • Modeling the mitochondrial cardiomyopathy of Barth syndrome with iPSC and heart-on-chip technologies 

      Wang, Gang; McCain, Megan L.; Yang, Luhan; He, Aibin; Pasqualini, Francesco Silvio; Agarwal, Ashutosh; Yuan, Hongyan; Jiang, Dawei; Zhang, Donghui; Zangi, Lior; Geva, Judith; Roberts, Amy E.; Ma, Qing; Ding, Jian; Chen, Jinghai; Wang, Da-zhi; Li, Kai; Wang, Jiwu; Wanders, Ronald J. A.; Kulik, Wim; Vaz, Frédéric M.; Laflamme, Michael A.; Murry, Charles E.; Chien, Kenneth R.; Kelley, Richard I.; Church, George M.; Parker, Kevin Kit; Pu, William T. (2014)
      Studying monogenic mitochondrial cardiomyopathies may yield insights into mitochondrial roles in cardiac development and disease. Here, we combine patient-derived and genetically engineered iPSCs with tissue engineering ...