Browsing by Author "Dove, Simon"
Now showing items 1-11 of 11
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A Conserved Pattern of Primer-Dependent Transcription Initiation in Escherichia coli and Vibrio cholerae Revealed by 5′ RNA-seq
Druzhinin, Sergey Y.; Tran, Ngat T.; Skalenko, Kyle S.; Goldman, Seth R.; Knoblauch, Jared G.; Dove, Simon L.; Nickels, Bryce E. (Public Library of Science, 2015)Transcription initiation that involves the use of a 2- to ~4-nt oligoribonucleotide primer, “primer-dependent initiation,” (PDI) has been shown to be widely prevalent at promoters of genes expressed during the stationary ... -
Deep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat shock and oxidative stress response
Balasubramanian, Deepak; Kumari, Hansi; Jaric, Melita; Fernandez, Mitch; Turner, Keith H.; Dove, Simon L.; Narasimhan, Giri; Lory, Stephen; Mathee, Kalai (Oxford University Press, 2013)Pathogenicity of Pseudomonas aeruginosa, a major cause of many acute and chronic human infections, is determined by tightly regulated expression of multiple virulence factors. Quorum sensing (QS) controls expression of ... -
Elucidating the virulence control network in Francisella tularensis
Levasseur, Kathryn (2014-06-06)The Gram-negative bacterium Francisella tularensis is the causative agent of tularemia and a model intracellular pathogen. It is also considered a potential bioweapon, as F. tularensis is highly infectious and has the ... -
Higher order oligomerization is required for H-NS family member MvaT to form gene-silencing nucleoprotein filament
Winardhi, Ricksen S.; Fu, Wenbo; Castang, Sandra; Li, Yanan; Dove, Simon L.; Yan, Jie (Oxford University Press, 2012)MvaT from Pseudomonas aeruginosa is a member of the histone-like nucleoid structuring protein (H-NS) family of nucleoid-associated proteins widely spread among Gram-negative bacteria that functions to repress the expression ... -
Identification of MltG as a Potential Terminase for Peptidoglycan Polymerization in Bacteria
Yunck, Rachel (2016-05-05)Bacterial cells are fortified against osmotic lysis by a cell wall made of peptidoglycan (PG). Synthases called penicillin-binding proteins (PBPs), the targets of penicillin and related antibiotics, polymerize the glycan ... -
A Novel AT-Rich DNA Recognition Mechanism for Bacterial Xenogeneic Silencer MvaT
Ding, Pengfei; McFarland, Kirsty A.; Jin, Shujuan; Tong, Grace; Duan, Bo; Yang, Ally; Hughes, Timothy R.; Liu, Jun; Dove, Simon L.; Navarre, William Wiley; Xia, Bin (Public Library of Science, 2015)Bacterial xenogeneic silencing proteins selectively bind to and silence expression from many AT rich regions of the chromosome. They serve as master regulators of horizontally acquired DNA, including a large number of ... -
Single-Molecule Study on Histone-Like Nucleoid-Structuring Protein (H-NS) Paralogue in Pseudomonas aeruginosa: MvaU Bears DNA Organization Mode Similarities to MvaT
Winardhi, Ricksen S.; Castang, Sandra; Dove, Simon L.; Yan, Jie (Public Library of Science, 2014)Pseudomonas aeruginosa contains two distinct members of H-NS family of nucleoid-structuring proteins: MvaT and MvaU. Together, these proteins bind to the same regions of the chromosome and function coordinately in the ... -
Small Molecule Control of Virulence Gene Expression in Francisella tularensis
Charity, James Carl; Blalock, LeeAnn T.; Costante-Hamm, Michelle M.; Kasper, Dennis Lee; Dove, Simon L. (Public Library of Science, 2009)In Francisella tularensis, the SspA protein family members MglA and SspA form a complex that associates with RNA polymerase (RNAP) to positively control the expression of virulence genes critical for the intramacrophage ... -
Structural Basis for Type VI Secretion Effector Recognition by a Cognate Immunity Protein
Li, Mo; Le Trong, Isolde; Carl, Mike A.; Larson, Eric T.; Chou, Seemay; De Leon, Justin A.; Dove, Simon L.; Stenkamp, Ronald E.; Mougous, Joseph D. (Public Library of Science, 2012)The type VI secretion system (T6SS) has emerged as an important mediator of interbacterial interactions. A T6SS from Pseudomonas aeruginosa targets at least three effector proteins, \(\underline t\)ype VI \(\underline ... -
Twin RNA Polymerase–associated Proteins Control Virulence Gene Expression in Francisella tularensis
Costante-Hamm, Michelle M; Balon, Emmy; Schneewind, Olaf; Charity, James Carl; Rubin, Eric Joseph; Boyd, Dana H.; Dove, Simon L. (Public Library of Science, 2007)The MglA protein is the only known regulator of virulence gene expression in Francisella tularensis, yet it is unclear how it functions. F. tularensis also contains an MglA-like protein called SspA. Here, we show that MglA ... -
Ubiquitous Promoter-Localization of Essential Virulence Regulators in Francisella tularensis
Ramsey, Kathryn M.; Osborne, Melisa L.; Vvedenskaya, Irina O.; Su, Cathy; Nickels, Bryce E.; Dove, Simon L. (Public Library of Science, 2015)Francisella tularensis is a Gram-negative bacterium whose ability to replicate within macrophages and cause disease is strictly dependent upon the coordinate activities of three transcription regulators called MglA, SspA, ...