Browsing by Author "Bernhardt, Thomas"
Now showing items 1-10 of 10
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The ABC's of Cell Division: Regulation of Peptidoglycan Amidase Activity during Cytokinesis in Escherichia coli
Yang, Desiree Choy (2012-09-07)The bacterial cell wall, composed of peptidoglycan (PG), is an essential component of the cell envelope. This macromolecular structure fortifies the cell membrane, determines cell shape, and helps prevent osmotic lysis. ... -
Bacterial Cell Wall Synthases Require Outer Membrane Lipoprotein Cofactors
Markovski, Monica (2012-09-12)To fortify their cytoplasmic membrane and protect it from osmotic rupture, most bacteria surround themselves with a peptidoglycan (PG) exoskeleton. The PG synthases that build this structure are called penicillin-binding ... -
Coordinate control of virulence gene expression in Francisella tularensis
Rohlfing, Amy Elizabeth (2015-05-19)Francisella tularensis is a Gram-negative, intracellular pathogen and the causative agent of tularemia. Due to its low infectious dose, ability to cause potentially fatal disease, and ability to be easily aerosolized, ... -
A Genome-Wide Screen for Bacterial Envelope Biogenesis Mutants Identifies a Novel Factor Involved in Cell Wall Precursor Metabolism
Paradis-Bleau, Catherine; Kritikos, George; Orlova, Katya; Typas, Athanasios; Bernhardt, Thomas G. (Public Library of Science, 2014)The cell envelope of Gram-negative bacteria is a formidable barrier that is difficult for antimicrobial drugs to penetrate. Thus, the list of treatments effective against these organisms is small and with the rise of new ... -
High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis
Meeske, Alexander J.; Rodrigues, Christopher D. A.; Brady, Jacqueline; Lim, Hoong Chuin; Bernhardt, Thomas G.; Rudner, David Z. (Public Library of Science, 2016)The differentiation of the bacterium Bacillus subtilis into a dormant spore is among the most well-characterized developmental pathways in biology. Classical genetic screens performed over the past half century identified ... -
Identification of the SlmA Active Site Responsible for Blocking Bacterial Cytokinetic Ring Assembly over the Chromosome
Cho, Hongbaek; Bernhardt, Thomas G. (Public Library of Science, 2013)Bacterial cells use chromosome-associated division inhibitors to help coordinate the processes of DNA replication and segregation with cytokinesis. SlmA from Escherichia coli, a member of the tetracycline repressor (TetR)–like ... -
Lipoprotein Activators Stimulate Escherichia coli Penicillin-Binding Proteins by Different Mechanisms
Lupoli, Tania J.; Lebar, Matthew; Markovski, Monica; Bernhardt, Thomas G.; Kahne, Daniel; Kahne, Suzanne Walker (American Chemical Society (ACS), 2014)In Escherichia coli, the bifunctional penicillin-binding proteins (PBPs), PBP1A and PBP1B, play critical roles in the final stage of peptidoglycan (PG) biosynthesis. These synthetic enzymes each possess a PG glycosyltransferase ... -
Mechanisms Regulating Target Selection and Degradation by Selective Autophagy
Kamber, Roarke Alexander (2016-09-12)Selective autophagy eliminates protein aggregates, damaged organelles, and other targets that otherwise accumulate and cause disease. A class of proteins called autophagy receptors is known to select targets for degradation, ... -
Molecular Dissection of the Essential Features of the Origin of Replication of the Second Vibrio Cholerae Chromosome
Gerding, Matthew (2015-05-16)Vibrionaceae family members are interesting models for studying DNA replication initiation as they contain two circular chromosomes. Chromosome II (chrII) replication is governed by two evolutionarily unique, yet highly ... -
New Genomics Tools and Strategies for Studying Antibiotics and Antibiotic-Resistance in Staphylococcus Aureus
Santiago, Marina Joy (2016-05-18)Staphylococcus aureus is a gram positive coccoid pathogen that causes intractable infections in hospitals and communities around the world, and tens of thousands of people die of these infections every year. In order to ...