Browsing by Author "Polyak, Kornelia"

Now showing items 1-18 of 18

    • Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells 

      Huh, Sung Jin; Clement, Kendell; Jee, David; Merlini, Alessandra; Choudhury, Sibgat; Maruyama, Reo; Yoo, Ronnie; Chytil, Anna; Boyle, Patrick; Ran, Fei Ann; Moses, Harold L.; Barcellos-Hoff, Mary Helen; Jackson-Grusby, Laurie; Meissner, Alexander; Polyak, Kornelia (Elsevier, 2015)
      Summary Postnatal mammary gland development and differentiation occur during puberty and pregnancy. To explore the role of DNA methylation in these processes, we determined the genome-wide DNA methylation and gene expression ...

    • BRCA1 haploinsufficiency for replication stress suppression in primary cells 

      Pathania, Shailja; Bade, Sangeeta; Le Guillou, Morwenna; Burke, Karly; Reed, Rachel; Bowman-Colin, Christian; Su, Ying; Ting, David T.; Polyak, Kornelia; Richardson, Andrea L.; Feunteun, Jean; Garber, Judy E.; Livingston, David M. (Nature Pub. Group, 2014)
      BRCA1—a breast and ovarian cancer suppressor gene—promotes genome integrity. To study the functionality of BRCA1 in the heterozygous state, we established a collection of primary human BRCA1+/+ and BRCA1mut/+ mammary ...

    • Dermcidin exerts its oncogenic effects in breast cancer via modulation of ERBB signaling 

      Bancovik, Jasna; Moreira, Dayson F; Carrasco, Daniel; Yao, Jun; Porter, Dale; Moura, Ricardo; Camargo, Anamaria; Fontes-Oliveira, Cibely C; Malpartida, Miguel G; Carambula, Silvia; Vannier, Edouard; Strauss, Bryan E; Wakamatsu, Alda; Alves, Venancio AF; Logullo, Angela F; Soares, Fernando A; Polyak, Kornelia; Belizário, José E (BioMed Central, 2015)
      Background: We previously identified dermicidin (DCD), which encodes a growth and survival factor, as a gene amplified and overexpressed in a subset of breast tumors. Patients with DCD-positive breast cancer have worse ...

    • Expression of estrogen receptor, progesterone receptor, and Ki67 in normal breast tissue in relation to subsequent risk of breast cancer 

      Oh, Hannah; Eliassen, A Heather; Wang, Molin; Smith-Warner, Stephanie A; Beck, Andrew H; Schnitt, Stuart J; Collins, Laura C; Connolly, James L; Montaser-Kouhsari, Laleh; Polyak, Kornelia; Tamimi, Rulla M (2016)
      Although expression of estrogen receptor (ER), progesterone receptor (PR), and cell proliferation marker Ki67 serve as predictive and prognostic factors in breast cancers, little is known about their roles in normal breast ...

    • The Expression of Psoriasin (S100A7) and CD24 Is Linked and Related to the Differentiation of Mammary Epithelial Cells 

      Vegfors, Jenny; Petersson, Stina; Kovács, Anikó; Polyak, Kornelia; Enerbäck, Charlotta (Public Library of Science, 2012)
      Psoriasin (S100A7), a member of the S100 family of calcium-binding proteins, is highly expressed in high-grade ductal carcinoma in situ (DCIS) and in the benign hyperproliferative skin disorder psoriasis. The gene that ...

    • Gene expression profiling of human breast tissue samples using SAGE-Seq 

      Wu, Z. J.; Meyer, Clifford; Choudhury, S.; Shipitsin, M.; Maruyama, R.; Bessarabova, M.; Nikolskaya, T.; Sukumar, S.; Schwartzman, A.; Liu, Jun; Polyak, Kornelia; Liu, Xiaole (Shirley) Shirley (Cold Spring Harbor Laboratory Press, 2010)
      We present a powerful application of ultra high-throughput sequencing, SAGE-Seq, for the accurate quantification of normal and neoplastic mammary epithelial cell transcriptomes. We develop data analysis pipelines that allow ...

    • In situ single cell analysis identifies heterogeneity for PIK3CA mutation and HER2 amplification in HER2+ breast cancer 

      Janiszewska, Michalina; Liu, Lin; Almendro, Vanessa; Kuang, Yanan; Paweletz, Cloud; Sakr, Rita A.; Weigelt, Britta; Hanker, Ariella B.; Chandarlapaty, Sarat; King, Tari A.; Reis-Filho, Jorge S.; Arteaga, Carlos L.; Park, So Yeon; Michor, Franziska; Polyak, Kornelia (2015)
      Detection of minor genetically distinct subpopulations within tumors is a key challenge in cancer genomics. Here we report STAR-FISH (Specific-To-Allele PCR – FISH), a novel method for the combined detection of single ...

    • An Intraductal Human-in-mouse Transplantation Model Mimics the Subtypes of Ductal Carcinoma In Situ 

      Behbod, Fariba; Kittrell, Frances S; LaMarca, Heather; Kerbawy, Sofia; Heestand, Jessica C; Young, Evelin; Mukhopadhyay, Purna; Yeh, Hung-Wen; Allred, D Craig; Medina, Daniel; Edwards, David; Hu, Min; Polyak, Kornelia; Rosen, Jeffrey M (BioMed Central, 2009)
      Introduction: Human models of noninvasive breast tumors are limited, and the existing in vivo models do not mimic inter- and intratumoral heterogeneity. Ductal carcinoma in situ (DCIS) is the most common type (80%) of ...

    • Mechanisms of Oncogenesis Driven by SNF5-Loss in Pediatric Rhabdoid Tumors 

      Lee, Ryan (2015-05-16)
      The characterization of inactivating mutations affecting SNF5 in pediatric rhabdoid tumors constituted the first clear connection between an epigenetic regulator and tumor formation. SNF5 is a core subunit of the evolutionarily ...

    • The Microenvironment in Breast Cancer Progression: Biology and Implications for Treatment 

      Place, Andrew Elliott; Huh, Sung Jin; Polyak, Kornelia (BioMed Central, 2011)
      Breast cancer comprises a heterogeneous group of malignancies derived from the ductal epithelium. The microenvironment of these cancers is now recognized as a critical participant in tumor progression and therapeutic ...

    • Molecular Determinants and Transcriptional Regulators in Triple Negative Breast Cancer 

      Subedee, Ashim (2015-08-07)
      Breast cancer is a highly heterogeneous disease with differences in histopathological and biological characteristics, variable prognoses, and response to therapy. Clinically, breast tumors are classified based on the ...

    • Non-cell autonomous tumor-growth driving supports sub-clonal heterogeneity 

      Marusyk, Andriy; Tabassum, Doris P.; Altrock, Philipp M.; Almendro, Vanessa; Michor, Franziska; Polyak, Kornelia (2014)
      SUMMARY Cancers arise through a process of somatic evolution that can result in substantial sub-clonal heterogeneity within tumors. The mechanisms responsible for the coexistence of distinct sub-clones and the biological ...

    • PAK1 is a Breast Cancer Oncogene that Coordinately Activates MAPK and MET Signaling 

      Shrestha, Yashaswi; Schafer, Eric J.; Boehm, Jesse S.; He, Frank; Wang, Shumei; Barretina, Jordi; Thomas, Sapana Rachael; Du, Jinyan; Weir, Barbara Ann; Zhao, Jean J.; Golub, Todd R.; Beroukhim, Rameen; Hahn, William C.; Polyak, Kornelia (Nature Publishing Group, 2012)
      Activating mutations in the RAS family or BRAF frequently occur in many types of human cancers but are rarely detected in breast tumors. However, activation of the RAS-RAF-MEK-ERK Mitogen-Activated Protein Kinase (MAPK) ...

    • Profiling Critical Cancer Gene Mutations in Clinical Tumor Samples 

      Campbell, Catarina D.; Kehoe, Sarah M.; Hatton, Charles; Niu, Lili; Yao, Keluo; Hanna, Megan; Mondal, Chandrani; Luongo, Lauren; Baker, Alissa C.; Philips, Juliet; Goff, Deborah J.; Rubin, Mark A.; Corso, Gianni; Roviello, Franco; MacConaill, Laura Eleanor; Bass, Adam Joel; Davis, Matthew J; Emery, Caroline Margaret; Fiorentino, Michelangelo; Polyak, Kornelia; Chan, Jennifer Ang; Wang, Yufang; Fletcher, Jonathan A.; Santagata, Sandro; Shivdasani, Ramesh Arjun; Kieran, Mark W.; Ligon, Keith Lloyd; Stiles, Charles Dean; Hahn, William C.; Meyerson, Matthew Langer; Garraway, Levi Alexander; Jones, Chris (Public Library of Science, 2009)
      Background: Detection of critical cancer gene mutations in clinical tumor specimens may predict patient outcomes and inform treatment options; however, high-throughput mutation profiling remains underdeveloped as a diagnostic ...

    • PTK6 Regulates IGF-1-Induced Anchorage-Independent Survival 

      Iida, Naoko; Zou, Lihua; Yao, Jun; Lu, Yiling; Epstein, Charles B.; Natesan, Sridaran; Mills, Gordon B.; Irie, Hanna Yoko; Shrestha, Yashaswi; Selfors, Laura Marie; Frye, Fabianne; Wang, Zhigang C.; Richardson, Andrea Lynn; Polyak, Kornelia; Hahn, William C.; Brugge, Joan S. (Public Library of Science, 2010)
      Background: Proteins that are required for anchorage-independent survival of tumor cells represent attractive targets for therapeutic intervention since this property is believed to be critical for survival of tumor cells ...

    • Response and resistance to BET bromodomain inhibitors in triple negative breast cancer 

      Shu, Shaokun; Lin, Charles Y.; He, Housheng Hansen; Witwicki, Robert M.; Tabassum, Doris P.; Roberts, Justin M.; Janiszewska, Michalina; Huh, Sung Jin; Liang, Yi; Ryan, Jeremy; Doherty, Ernest; Mohammed, Hisham; Guo, Hao; Stover, Daniel G.; Ekram, Muhammad B.; Brown, Jonathan; D'Santos, Clive; Krop, Ian E.; Dillon, Deborah; McKeown, Michael; Ott, Christopher; Qi, Jun; Ni, Min; Rao, Prakash K.; Duarte, Melissa; Wu, Shwu-Yuan; Chiang, Cheng-Ming; Anders, Lars; Young, Richard A.; Winer, Eric; Letai, Antony; Barry, William T.; Carroll, Jason S.; Long, Henry; Brown, Myles; Liu, X. Shirley; Meyer, Clifford A.; Bradner, James E.; Polyak, Kornelia (2015)
      Triple negative breast cancer (TNBC) is a heterogeneous and clinically aggressive disease for which there is no targeted therapy1-3. BET bromodomain inhibitors, which have shown efficacy in several models of cancer4-6, ...

    • RNA sequencing of cancer reveals novel splicing alterations 

      Eswaran, Jeyanthy; Horvath, Anelia; Godbole, Sucheta; Reddy, Sirigiri Divijendra; Mudvari, Prakriti; Ohshiro, Kazufumi; Cyanam, Dinesh; Nair, Sujit; Fuqua, Suzanne A. W.; Polyak, Kornelia; Florea, Liliana D.; Kumar, Rakesh (Nature Publishing Group, 2013)
      Breast cancer transcriptome acquires a myriad of regulation changes, and splicing is critical for the cell to “tailor-make” specific functional transcripts. We systematically revealed splicing signatures of the three most ...

    • S100A7-Downregulation Inhibits Epidermal Growth Factor-Induced Signaling in Breast Cancer Cells and Blocks Osteoclast Formation 

      Paruchuri, Vikram; Prasad, Anil; McHugh, Kevin P.; Bhat, Hari K.; Polyak, Kornelia; Ganju, Ramesh K. (Public Library of Science, 2008)
      S100A7 is a small calcium binding protein, which has been shown to be differentially expressed in psoriatic skin lesions, as well as in squamous cell tumors of the skin, lung and breast. Although its expression has been ...