Browsing by Author "Tsankov, Alexander M."

Now showing items 1-6 of 6

    • Epigenomics and Chromatin Dynamics 

      Akopian, Veronika; Chan, Michelle; Clement, Kendell; Galonska, Christina; Gifford, Casey; Lehtola, Elizabeth; Liao, Jing; Samavarchi-Tehrani, Payman; Sindhu, Camille; Smith, Zachary D; Tsankov, Alexander; Webster, Jamie Orme; Zhang, Yingying; Ziller, Michael Johannes; Meissner, Alexander (BioMed Central, 2012)
      A report of the 'Joint Keystone Symposium on Epigenomics and Chromatin Dynamics', Keystone, Colorado, 17-22 January 2012. This year's Joint Keystone Symposium on Epigenomics and Chromatin Dynamics was one of the largest ...

    • An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells 

      Tsankov, Alexander M.; Akopian, Veronika; Pop, Ramona; Chetty, Sundari; Gifford, Casey A.; Daheron, Laurence; Melton, Douglas A.; Tsankova, Nadejda M.; Meissner, Alexander (2015)
      Research on human pluripotent stem cells has been hampered by the lack of a standardized, quantitative, scalable assay of pluripotency. We have previously described an assay called ScoreCard that used gene expression ...

    • A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation 

      Chetty, Sundari; Engquist, Elise N.; Mehanna, Elie; Lui, Kathy O.; Tsankov, Alexander M.; Melton, Douglas A. (The Rockefeller University Press, 2015)
      Driving human pluripotent stem cells (hPSCs) into specific lineages is an inefficient and challenging process. We show that a potent Src inhibitor, PP1, regulates expression of genes involved in the G1 to S phase transition ...

    • Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells 

      Liao, Jing; Karnik, Rahul; Gu, Hongcang; Ziller, Michael J.; Clement, Kendell; Tsankov, Alexander M.; Akopian, Veronika; Gifford, Casey A.; Donaghey, Julie; Galonska, Christina; Pop, Ramona; Reyon, Deepak; Tsai, Shengdar Q.; Mallard, William; Joung, J. Keith; Rinn, John L.; Gnirke, Andreas; Meissner, Alexander (2015)
      DNA methylation is a key epigenetic modification involved in regulating gene expression and maintaining genomic integrity. Here we inactivated all three catalytically active DNA methyltransferases in human embryonic stem ...

    • Transcription factor binding dynamics during human ESC differentiation 

      Tsankov, Alexander M.; Gu, Hongcang; Akopian, Veronika; Ziller, Michael J.; Donaghey, Julie; Amit, Ido; Gnirke, Andreas; Meissner, Alexander (2015)
      Summary Pluripotent stem cells provide a powerful system to dissect the underlying molecular dynamics that regulate cell fate changes during mammalian development. Here we report the integrative analysis of genome wide ...

    • Transcriptional and Epigenetic Dynamics during Specification of Human Embryonic Stem Cells 

      Gifford, Casey A.; Ziller, Michael Johannes; Gu, Hongcang; Trapnell, Cole; Donaghey, Julie A; Tsankov, Alexander; Shalek, Alex K.; Kelley, David Roy; Shishkin, Alexander A.; Issner, Robbyn; Zhang, Xiaolan; Coyne, Michael J.; Fostel, Jennifer L.; Holmes, Laurie; Meldrim, Jim; Guttman, Mitchell; Epstein, Charles; Park, Hongkun; Kohlbacher, Oliver; Rinn, John L; Gnirke, Andreas; Lander, Eric Steven; Bernstein, Bradley E.; Meissner, Alexander (Elsevier BV, 2013)
      Differentiation of human embryonic stem cells (hESCs) provides a unique opportunity to study the regulatory mechanisms that facilitate cellular transitions in a human context. To that end, we performed comprehensive ...