Now showing items 1-4 of 4

    • Charting a dynamic DNA methylation landscape of the human genome 

      Ziller, Michael J.; Gu, Hongcang; Müller, Fabian; Donaghey, Julie; Tsai, Linus T.-Y.; Kohlbacher, Oliver; De Jager, Phil L.; Rosen, Evan D.; Bennett, David A.; Bernstein, Bradley E.; Gnirke, Andreas; Meissner, Alexander (2013)
      DNA methylation is a defining feature of mammalian cellular identity and essential for normal development1,2. Most cell types, except germ cells and pre-implantation embryos3–5, display relatively stable DNA methylation ...
    • Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells 

      Liao, Jing; Karnik, Rahul; Gu, Hongcang; Ziller, Michael J.; Clement, Kendell; Tsankov, Alexander M.; Akopian, Veronika; Gifford, Casey A.; Donaghey, Julie; Galonska, Christina; Pop, Ramona; Reyon, Deepak; Tsai, Shengdar Q.; Mallard, William; Joung, J. Keith; Rinn, John L.; Gnirke, Andreas; Meissner, Alexander (2015)
      DNA methylation is a key epigenetic modification involved in regulating gene expression and maintaining genomic integrity. Here we inactivated all three catalytically active DNA methyltransferases in human embryonic stem ...
    • Transcription factor binding dynamics during human ESC differentiation 

      Tsankov, Alexander M.; Gu, Hongcang; Akopian, Veronika; Ziller, Michael J.; Donaghey, Julie; Amit, Ido; Gnirke, Andreas; Meissner, Alexander (2015)
      Summary Pluripotent stem cells provide a powerful system to dissect the underlying molecular dynamics that regulate cell fate changes during mammalian development. Here we report the integrative analysis of genome wide ...
    • Transcriptional and Epigenetic Dynamics during Specification of Human Embryonic Stem Cells 

      Gifford, Casey A.; Ziller, Michael Johannes; Gu, Hongcang; Trapnell, Cole; Donaghey, Julie A; Tsankov, Alexander; Shalek, Alex K.; Kelley, David Roy; Shishkin, Alexander A.; Issner, Robbyn; Zhang, Xiaolan; Coyne, Michael J.; Fostel, Jennifer L.; Holmes, Laurie; Meldrim, Jim; Guttman, Mitchell; Epstein, Charles; Park, Hongkun; Kohlbacher, Oliver; Rinn, John L; Gnirke, Andreas; Lander, Eric Steven; Bernstein, Bradley E.; Meissner, Alexander (Elsevier BV, 2013)
      Differentiation of human embryonic stem cells (hESCs) provides a unique opportunity to study the regulatory mechanisms that facilitate cellular transitions in a human context. To that end, we performed comprehensive ...