Now showing items 1-10 of 10

    • Crossover recombination mediated by HIM-18/SLX4-associated nucleases 

      Saito, Takamune T; Colaiácovo, Monica P (Landes Bioscience, 2014)
      Meiosis is a specialized cell division program that results in the formation of haploid gametes (i.e., sperm and eggs) from diploid parental cells, and is essential for all sexually reproducing organisms. Crossover formation, ...
    • A Genetic Screen Identifies FAN1, a Fanconi Anemia-Associated Nuclease Necessary for DNA Interstrand Crosslink Repair 

      Smogorzewska, Agata; Desetty, Rohini; Saito, Takamune; Schlabach, Michael; Lach, Francis P.; Sowa, Mathew E.; Clark, Alan B.; Kunkel, Thomas A.; Harper, Jeffrey Wade; Colaiacovo, Monica P.; Elledge, Stephen J. (Elsevier BV, 2010)
      The Fanconi anemia (FA) pathway is responsible for interstrand crosslink repair. At the heart of this pathway is the FANCI-FAND2 (ID) complex, which, upon ubiquitination by the FA core complex, travels to sites of damage ...
    • Heritable genome editing in C. elegans via a CRISPR-Cas9 system 

      Friedland, Ari E.; Tzur, Yonatan B.; Esvelt, Kevin M.; Colaiácovo, Monica P.; Church, George M.; Calarco, John A. (2013)
      CRISPR-Cas systems have been used with single-guide RNAs for accurate gene disruption and conversion in multiple biological systems. Here we report the use of the endonuclease Cas9 to target genomic sequences in the C. ...
    • Interplay between Structure-Specific Endonucleases for Crossover Control during Caenorhabditis elegans Meiosis 

      Saito, Takamune T.; Lui, Doris Y.; Kim, Hyun-Min; Meyer, Katherine; Colaiácovo, Monica P. (Public Library of Science, 2013)
      The number and distribution of crossover events are tightly regulated at prophase of meiosis I. The resolution of Holliday junctions by structure-specific endonucleases, including MUS-81, SLX-1, XPF-1 and GEN-1, is one of ...
    • Investigating the Role of RIO Protein Kinases in Caenorhabditis elegans 

      Mendes, Tasha K.; Novakovic, Stevan; Raymant, Greta; Bertram, Sonja E.; Esmaillie, Reza; Nadarajan, Saravanapriah; Breugelmans, Bert; Hofmann, Andreas; Gasser, Robin B.; Colaiácovo, Monica P.; Boag, Peter R. (Public Library of Science, 2015)
      RIO protein kinases (RIOKs) are a relatively conserved family of enzymes implicated in cell cycle control and ribosomal RNA processing. Despite their functional importance, they remain a poorly understood group of kinases ...
    • LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion 

      Egydio de Carvalho, Carlos; Van Bostelen, Ivo; Gu, Yanjie; Chu, Diana S.; Cheeseman, Iain M.; Tzur, Yonatan B; Nadarajan, Saravanapriah; Colaiacovo, Monica P. (Public Library of Science, 2012)
      Successful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific ...
    • The MAP kinase pathway coordinates crossover designation with disassembly of synaptonemal complex proteins during meiosis 

      Nadarajan, Saravanapriah; Mohideen, Firaz; Tzur, Yonatan B; Ferrandiz, Nuria; Crawley, Oliver; Montoya, Alex; Faull, Peter; Snijders, Ambrosius P; Cutillas, Pedro R; Jambhekar, Ashwini; Blower, Michael D; Martinez-Perez, Enrique; Harper, J Wade; Colaiacovo, Monica P (eLife Sciences Publications, Ltd, 2016)
      Asymmetric disassembly of the synaptonemal complex (SC) is crucial for proper meiotic chromosome segregation. However, the signaling mechanisms that directly regulate this process are poorly understood. Here we show that ...
    • NatB Domain-Containing CRA-1 Antagonizes Hydrolase ACER-1 Linking Acetyl-CoA Metabolism to the Initiation of Recombination during C. elegans Meiosis 

      Gao, Jinmin; Kim, Hyun-Min; Elia, Andrew E.; Elledge, Stephen J.; Colaiácovo, Monica P. (Public Library of Science, 2015)
      The formation of DNA double-strand breaks (DSBs) must take place during meiosis to ensure the formation of crossovers, which are required for accurate chromosome segregation, therefore avoiding aneuploidy. However, DSB ...
    • REST and Stress Resistance in Ageing and Alzheimer’s Disease 

      Lu, Tao; Aron, Liviu; Zullo, Joseph M.; Pan, Ying; Kim, Haeyoung; Chen, Yiwen; Yang, Tun-Hsiang; Kim, Hyun-Min; Drake, Derek Mark; Liu, Xiaole (Shirley) Shirley; Bennett, David A.; Colaiacovo, Monica P.; Yankner, Bruce Albert (Nature Publishing Group, 2014)
      Human neurons are functional over an entire lifetime, yet the mechanisms that preserve function and protect against neurodegeneration during ageing are unknown. Here we show that induction of the repressor element 1-silencing ...
    • ZTF-8 Interacts with the 9-1-1 Complex and Is Required for DNA Damage Response and Double-Strand Break Repair in the C. elegans Germline 

      Kim, Hyun-Min; Colaiácovo, Monica P. (Public Library of Science, 2014)
      Germline mutations in DNA repair genes are linked to tumor progression. Furthermore, failure in either activating a DNA damage checkpoint or repairing programmed meiotic double-strand breaks (DSBs) can impair chromosome ...