Browsing by Author "Ramirez, Cherie Lynn"
Now showing items 1-4 of 4
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Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects
Ramirez, Cherie Lynn; Certo, Michael T.; Mussolino, Claudio; Goodwin, Matthew J.; Cradick, Thomas J.; McCaffrey, Anton P.; Cathomen, Toni; Scharenberg, Andrew M.; Joung, Jae Keith (Oxford University Press, 2012)Engineered zinc finger nucleases (ZFNs) induce DNA double-strand breaks at specific recognition sequences and can promote efficient introduction of desired insertions, deletions or substitutions at or near the cut site via ... -
Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs)
Moore, Finola Elizabeth; Reyon, Deepak; Sander, Jeffry D.; Martinez, Sarah A.; Blackburn, Jessica S.; Khayter, Cyd; Ramirez, Cherie Lynn; Joung, Jae Keith; Langenau, David M. (Public Library of Science, 2012)Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in ... -
Improving Zinc Finger Nucleases - Strategies for Increasing Gene Editing Activities and Evaluating Off-Target Effects
Ramirez, Cherie Lynn (2012-08-23)Zinc finger nucleases (ZFNs) induce double-strand DNA breaks at specific recognition sites. ZFNs can dramatically increase the efficiency of incorporating desired insertions, deletions, or substitutions in living cells. ... -
In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites
Sander, Jeffry D.; Ramirez, Cherie L.; Linder, Samantha J.; Pattanayak, Vikram; Shoresh, Noam; Ku, Manching; Foden, Jennifer A.; Reyon, Deepak; Bernstein, Bradley E.; Liu, David R.; Joung, J. Keith (Oxford University Press, 2013)Gene-editing nucleases enable targeted modification of DNA sequences in living cells, thereby facilitating efficient knockout and precise editing of endogenous loci. Engineered nucleases also have the potential to introduce ...