Browsing by Author "Murray, Andrew"
Now showing items 1-20 of 29
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Asymmetry in Sexual Pheromones Is Not Required for Ascomycete Mating
Gonçalves-Sá, Joana; Murray, Andrew W. (Elsevier BV, 2011)Highlights Asymmetric modification of pheromones is not required for yeast mating Two yeast strains that express complementary pheromones and receptors mate with each other Two yeast strains that express the ... -
Chromosomal attachments set length and microtubule number in the Saccharomyces cerevisiae mitotic spindle
Nannas, Natalie J.; O’Toole, Eileen T.; Winey, Mark; Murray, Andrew W. (The American Society for Cell Biology, 2014)The length of the mitotic spindle varies among different cell types. A simple model for spindle length regulation requires balancing two forces: pulling, due to microtubules that attach to the chromosomes at their ... -
Complications Dawn for Kinetochore Regulation by Aurora
Nannas, Natalie; Murray, Andrew W. (Proceedings of the National Academy of Sciences, 2012)Organisms must faithfully segregate their chromosomes during cell division; mistakes in this process can be costly and even fatal to the organism (1, 2). During mitosis, replicated chromosomes attach to the spindle, a ... -
Conservation Weighting Functions Enable Covariance Analyses to Detect Functionally Important Amino Acids
Colwell, Lucy J.; Brenner, Michael P.; Murray, Andrew W. (Public Library of Science, 2014)The explosive growth in the number of protein sequences gives rise to the possibility of using the natural variation in sequences of homologous proteins to find residues that control different protein phenotypes. Because ... -
The Cost of Gene Expression Underlies a Fitness Trade-Off in Yeast
Lang, Gregory I.; Murray, Andrew W.; Botstein, David (National Academy of Sciences, 2009)Natural selection optimizes an organism's genotype within the context of its environment. Adaptations to one environment can decrease fitness in another, revealing evolutionary trade-offs. Here, we show that the cost of ... -
Dissecting Protein-Protein Interactions that Regulate the Spindle Checkpoint in Budding Yeast
Lau, Tsz Cham Derek (2013-03-05)Errors in segregation of genetic materials are detrimental to all organisms. The budding yeast ensures accurate chromosome segregation by employing a system called the spindle checkpoint. The spindle checkpoint, which ... -
Genetically Engineered Transvestites Reveal Novel Mating Genes in Budding Yeast
Huberman, Lori Bromer; Murray, Andrew W. (The Genetics Society of America, 2013)Haploid budding yeast has two mating types, defined by the alleles of the MAT locus, MATa and MATα. Two haploid cells of opposite mating types mate by signaling to each other using reciprocal pheromones and receptors, ... -
High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast
Segrè, Ayellet V; Murray, Andrew W.; Leu, Jun-Yi (Public Library of Science, 2006)Understanding the genetic basis of evolutionary adaptation is limited by our ability to efficiently identify the genomic locations of adaptive mutations. Here we describe a method that can quickly and precisely map the ... -
How Obstacles Perturb Population Fronts and Alter Their Genetic Structure
Möbius, Wolfram; Murray, Andrew W.; Nelson, David R. (Public Library of Science, 2015)As populations spread into new territory, environmental heterogeneities can shape the population front and genetic composition. We focus here on the effects of an important building block of heterogeneous environments, ... -
Improved Use of a Public Good Selects for the Evolution of Undifferentiated Multicellularity
Koschwanez, John H; Foster, Kevin R; Murray, Andrew W. (eLife Sciences Publications, Ltd, 2013)We do not know how or why multicellularity evolved. We used the budding yeast, Saccharomyces cerevisiae, to ask whether nutrients that must be digested extracellularly select for the evolution of undifferentiated ... -
Investigation of Force, Kinetochores, and Tension in the Saccharomyces Cerevisiae Mitotic Spindle
Nannas, Natalie Jo (2013-08-14)Cells must faithfully segregate their chromosomes at division; errors in this process causes cells to inherit an incorrect number of chromosomes, a hallmark of birth defects and cancer. The machinery required to segregate ... -
Mad2 and Mad3 Cooperate to Arrest Budding Yeast in Mitosis
Lau, Derek T. C.; Murray, Andrew W. (Elsevier, 2012)Background: The spindle checkpoint ensures accurate chromosome transmission by delaying chromosome segregation until all chromosomes are correctly aligned on the mitotic spindle. The checkpoint is activated by kinetochores ... -
Mechanisms of Transcriptional Control in Phosphate-responsive Signaling Pathway of Saccharomyces cerevisiae
Zhou, Xu (2013-10-08)Regulation of gene expression is essential for many biological processes. Binding of transcription factors to DNA is a key regulatory step in the control of gene expression. It is commonly observed that DNA sequences ... -
A Model for Cell Wall Dissolution in Mating Yeast Cells: Polarized Secretion and Restricted Diffusion of Cell Wall Remodeling Enzymes Induces Local Dissolution
Huberman, Lori B.; Murray, Andrew W. (Public Library of Science, 2014)Mating of the budding yeast, Saccharomyces cerevisiae, occurs when two haploid cells of opposite mating types signal using reciprocal pheromones and receptors, grow towards each other, and fuse to form a single diploid ... -
A Model for the Evolution of Biological Specificity: a Cross-Reacting DNA-Binding Protein Causes Plasmid Incompatibility
Hyland, E. M.; Wallace, E. W. J.; Murray, Andrew W. (American Society for Microbiology, 2014)Few biological systems permit rigorous testing of how changes in DNA sequence give rise to adaptive phenotypes. In this study, we sought a simplified experimental system with a detailed understanding of the genotype-to-phenotype ... -
Non-Canonical Translation in Vertebrates
Chew, Guo-Liang (2015-05-18)Translation is a key process during gene expression: to produce proteins, ribosomes translate the coding sequences of mRNAs. However, vertebrate genomes contain more translation potential than these annotated coding ... -
A Predictive Model for Yeast Cell Polarization in Pheromone Gradients
Muller, Nicolas; Piel, Matthieu; Calvez, Vincent; Voituriez, Raphaël; Gonçalves-Sá, Joana; Guo, Chin-Lin; Jiang, Xingyu; Murray, Andrew; Meunier, Nicolas (Public Library of Science, 2016)Budding yeast cells exist in two mating types, a and α, which use peptide pheromones to communicate with each other during mating. Mating depends on the ability of cells to polarize up pheromone gradients, but cells also ... -
Rapid Expansion and Functional Divergence of Subtelomeric Gene Families in Yeasts
Brown, Chris A.; Murray, Andrew W.; Verstrepen, Kevin J. (Elsevier BV, 2010)Background—Subtelomeres, regions proximal to telomeres, exhibit characteristics unique to eukaryotic genomes. Genes residing in these loci are subject to epigenetic regulation and elevated rates of both meiotic and mitotic ... -
Rapid hierarchical assembly of medium-size DNA cassettes
Schmid-Burgk, Jonathan Leo; Xie, Zhen; Frank, Stefan; Winter, Sebastian Virreira; Mitschka, Sibylle; Kolanus, Waldemar; Murray, Andrew W.; Benenson, Yaakov (Oxford University Press (OUP), 2012) -
Recruiting a Microtubule-Binding Complex to DNA Directs Chromosome Segregation in Budding Yeast
Lacefield, Soni; Lau, Tsz Cham Derek; Murray, Andrew W. (Natur Publishing Group, 2009)Accurate chromosome segregation depends on the kinetochore, the complex of proteins that link microtubules to centromeric DNA1. The budding yeast kinetochore consists of more than 80 proteins assembled on a 125bp region ...