Now showing items 1-10 of 10

    • Assembly Line Polyketide Synthases: Mechanistic Insights and Unsolved Problems 

      Khosla, Chaitan; Herschlag, Daniel; Cane, David E.; Walsh, Christopher T. (American Chemical Society, 2014)
      Two hallmarks of assembly line polyketide synthases have motivated an interest in these unusual multienzyme systems, their stereospecificity and their capacity for directional biosynthesis. In this review, we summarize the ...
    • The biosynthesis of cyanobacterial sunscreen scytonemin in intertidal microbial mat communities 

      Balskus, Emily Patricia; Case, Rebecca J.; Walsh, Christopher T. (Wiley-Blackwell, 2011)
      We have examined the biosynthesis and accumulation of cyanobacterial sunscreening pigment scytonemin within intertidal microbial mat communities using a combination of chemical, molecular, and phylogenetic approaches. Both ...
    • Crystal structure of O-methyltransferase CalO6 from the calicheamicin biosynthetic pathway: a case of challenging structure determination at low resolution 

      Tsodikov, Oleg V.; Hou, Caixia; Walsh, Christopher T.; Garneau-Tsodikova, Sylvie (BioMed Central, 2015)
      Background: Calicheamicins (CAL) are enedyine natural products with potent antibiotic and cytotoxic activity, used in anticancer therapy. The O-methyltransferase CalO6 is proposed to catalyze methylation of the hydroxyl ...
    • Cyclization of Fungal Nonribosomal Peptides by a Terminal Condensation-Like Domain 

      Gao, Xue; Haynes, Stuart W.; Ames, Brian D.; Wang, Peng; Vien, Linda P.; Walsh, Christopher T.; Tang, Yi (2012)
      Cyclization of linear peptidyl precursors produced by nonribosomal peptide synthetases (NRPSs) is an important step in the biosynthesis of bioactive cyclic peptides. Whereas bacterial NRPSs use thioesterase (TE) domains ...
    • An Enzymatic Cyclopentyl[b]indole Formation Involved in Scytonemin Biosynthesis 

      Balskus, Emily Patricia; Walsh, Christopher T. (American Chemical Society (ACS), 2009)
      Previous studies of the biosynthetic enzymes involved in the assembly of scytonemin (1), a cyanobacterial sunscreen, have identified β-ketoacid 2 as an important intermediate that is produced by ThDP-dependent enzyme ScyA. ...
    • Enzymatic Glycosylation of Vancomycin Aglycon: Completion of a Total Synthesis of Vancomycin and N- and C-Terminus Substituent Effects of the Aglycon Substrate 

      Nakayama, Atsushi; Okano, Akinori; Feng, Yiqing; Collins, James C.; Collins, Karen C.; Walsh, Christopher T.; Boger, Dale L. (American Chemical Society, 2014)
      Studies on the further development of the sequential glycosylations of the vancomycin aglycon catalyzed by the glycosyltransferases GtfE and GtfD and the observation of unusual, perhaps unexpected, aglycon substrate ...
    • The FAD Cofactor of RebC Shifts to an IN Conformation upon Flavin Reduction†,‡ 

      Ryan, Katherine S.; Chakraborty, Sumita; Howard-Jones, Annaleise R.; Walsh, Christopher T.; Ballou, David P.; Drennan, Catherine L. (American Chemical Society, 2008)
      RebC is a putative flavin hydroxylase functioning together with RebP to carry out a key step in the biosynthesis of rebeccamycin. To probe the mechanism of flavin-based chemistry in RebC, we solved the structure of RebC ...
    • The Genetic and Molecular Basis for Sunscreen Biosynthesis in Cyanobacteria 

      Balskus, Emily Patricia; Walsh, Christopher T. (American Association for the Advancement of Science (AAAS), 2010)
      Ultraviolet UV-A and UV-B radiation is harmful to living systems, causing damage to biological macromolecules. An important strategy for dealing with UV exposure is the biosynthesis of small-molecule sunscreens. Among such ...
    • Investigating the Initial Steps in the Biosynthesis of Cyanobacterial Sunscreen Scytonemin 

      Balskus, Emily Patricia; Walsh, Christopher T. (American Chemical Society (ACS), 2008)
      The cyanobacterial natural product scytonemin (1) functions as a sunscreen, absorbing harmful UV-A radiation. Using information from a recently identified gene cluster, we propose a biosynthetic route to this pigment. We ...
    • Minimum Information about a Biosynthetic Gene cluster 

      Medema, Marnix H; Kottmann, Renzo; Yilmaz, Pelin; Cummings, Matthew; Biggins, John B; Blin, Kai; de Bruijn, Irene; Chooi, Yit Heng; Claesen, Jan; Coates, R Cameron; Cruz-Morales, Pablo; Duddela, Srikanth; Düsterhus, Stephanie; Edwards, Daniel J; Fewer, David P; Garg, Neha; Geiger, Christoph; Gomez-Escribano, Juan Pablo; Greule, Anja; Hadjithomas, Michalis; Haines, Anthony S; Helfrich, Eric J N; Hillwig, Matthew L; Ishida, Keishi; Jones, Adam C; Jones, Carla S; Jungmann, Katrin; Kegler, Carsten; Kim, Hyun Uk; Kötter, Peter; Krug, Daniel; Masschelein, Joleen; Melnik, Alexey V; Mantovani, Simone M; Monroe, Emily A; Moore, Marcus; Moss, Nathan; Nützmann, Hans-Wilhelm; Pan, Guohui; Pati, Amrita; Petras, Daniel; Reen, F Jerry; Rosconi, Federico; Rui, Zhe; Tian, Zhenhua; Tobias, Nicholas J; Tsunematsu, Yuta; Wiemann, Philipp; Wyckoff, Elizabeth; Yan, Xiaohui; Yim, Grace; Yu, Fengan; Xie, Yunchang; Aigle, Bertrand; Apel, Alexander K; Balibar, Carl J; Balskus, Emily Patricia; Barona-Gómez, Francisco; Bechthold, Andreas; Bode, Helge B; Borriss, Rainer; Brady, Sean F; Brakhage, Axel A; Caffrey, Patrick; Cheng, Yi-Qiang; Clardy, Jon C.; Cox, Russell J; De Mot, René; Donadio, Stefano; Donia, Mohamed S; van der Donk, Wilfred A; Dorrestein, Pieter C; Doyle, Sean; Driessen, Arnold J M; Ehling-Schulz, Monika; Entian, Karl-Dieter; Fischbach, Michael A; Gerwick, Lena; Gerwick, William H; Gross, Harald; Gust, Bertolt; Hertweck, Christian; Höfte, Monica; Jensen, Susan E; Ju, Jianhua; Katz, Leonard; Kaysser, Leonard; Klassen, Jonathan L; Keller, Nancy P; Kormanec, Jan; Kuipers, Oscar P; Kuzuyama, Tomohisa; Kyrpides, Nikos C; Kwon, Hyung-Jin; Lautru, Sylvie; Lavigne, Rob; Lee, Chia Y; Linquan, Bai; Liu, Xinyu; Liu, Wen; Luzhetskyy, Andriy; Mahmud, Taifo; Mast, Yvonne; Méndez, Carmen; Metsä-Ketelä, Mikko; Micklefield, Jason; Mitchell, Douglas A; Moore, Bradley S; Moreira, Leonilde M; Müller, Rolf; Neilan, Brett A; Nett, Markus; Nielsen, Jens; O, Fergal; Oikawa, Hideaki; Osbourn, Anne; Osburne, Marcia S; Ostash, Bohdan; Payne, Shelley M; Pernodet, Jean-Luc; Petricek, Miroslav; Piel, Jörn; Ploux, Olivier; Raaijmakers, Jos M; Salas, José A; Schmitt, Esther K; Scott, Barry; Seipke, Ryan F; Shen, Ben; Sherman, David; Sivonen, Kaarina; Smanski, Michael J; Sosio, Margherita; Stegmann, Evi; Süssmuth, Roderich D; Tahlan, Kapil; Thomas, Christopher M; Tang, Yi; Truman, Andrew W; Viaud, Muriel; Walton, Jonathan D; Walsh, Christopher T.; Weber, Tilmann; van Wezel, Gilles P; Wilkinson, Barrie; Willey, Joanne M; Wohlleben, Wolfgang; Wright, Gerard D; Ziemert, Nadine; Zhang, Changsheng; Zotchev, Sergey B; Breitling, Rainer; Takano, Eriko; Glöckner, Frank Oliver (Springer Nature, 2015)
      A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded in biosynthetic gene clusters. Information about these clusters, pathways and metabolites is ...