Browsing by Author "Chapman, Alec"

Now showing items 1-6 of 6

    • Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell 

      Zong, Chenghang; Chapman, Alec Randolph; Xie, Xiaoliang Sunney (American Association for the Advancement of Science, 2012)
      Kindred cells can have different genomes because of dynamic changes in DNA. Single cell sequencing is needed to characterize these genomic differences but has been hindered by whole-genome amplification bias, resulting in ...

    • Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing 

      Lu, Sijia; Zong, Chenghang; Fan, Wei; Yang, Mingyu; Li, Jinsen; Chapman, Alec Randolph; Zhu, Ping; Hu, Xuesong; Xu, Liya; Yan, Liying; Bai, Fan; Qiao, Jie; Tang, Fuchou; Li, Ruiqiang; Xie, Xiaoliang Sunney (American Association for the Advancement of Science, 2012)
      Meiotic recombination creates genetic diversity and ensures segregation of homologous chromosomes. Previous population analyses yielded results averaged among individuals and affected by evolutionary pressures. We sequenced ...

    • Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients 

      Ni, Xiaohui; Zhuo, Minglei; Su, Zhe; Duan, J.; Gao, Yan; Wang, Z.; Zong, Chenghang; Bai, H.; Chapman, Alec Randolph; Zhao, J.; Xu, L.; An, T.; Ma, Q.; Wang, Y.; Wu, M.; Sun, Y.; Wang, S.; Li, Z.; Yang, X.; Yong, Jun; Su, X.-D.; Lu, Y.; Bai, F.; Xie, Xiaoliang Sunney; Wang, J. (Proceedings of the National Academy of Sciences, 2013)
      Circulating tumor cells (CTCs) enter peripheral blood from primary tumors and seed metastases. The genome sequencing of CTCs could offer noninvasive prognosis or even diagnosis, but has been hampered by low single-cell ...

    • Single Cell Transcriptome Amplification with MALBAC 

      Chapman, Alec Randolph; He, Hertz Zi; Sijia, Lu; Jun, Young; Longzhi, Tan; Fuchou, X Tang; Sunney, Xie (Public Library of Science (PLoS), 2015)
      Recently, Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) has been developed for whole genome amplification of an individual cell, relying on quasilinear instead of exponential amplification to achieve ...

    • Single Molecule Imaging of Transcription Factor Binding to DNA in Live Mammalian Cells 

      Gebhardt, Johann Christof manuel; Suter, David Michael; Roy, Rahul; Zhao, Zingqing W.; Chapman, Alec Randolph; Basu, Srinjan; Maniatis, Thomas P.; Xie, Xiaoliang Sunney (Nature Publishing Group, 2013)
      Imaging single fluorescent proteins in living mammalian cells is challenged by out-of-focus fluorescence excitation. To reduce out-of-focus fluorescence we developed reflected light-sheet microscopy (RLSM), a fluorescence ...

    • Spatial organization of RNA polymerase II inside a mammalian cell nucleus revealed by reflected light-sheet superresolution microscopy 

      Zhao, Ziqing; Xie, Xiaoliang Sunney; Roy, Rahul; Gebhardt, J. Christof M.; Suter, David M.; Chapman, Alec R. (2015-08-07)
      Superresolution microscopy based on single-molecule centroid determination has been widely applied to cellular imaging in recent years. However, quantitative imaging of the mammalian nucleus has been challenging due to the ...