Browsing by Author "Porichis, Filippos"
Now showing items 1-3 of 3
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Co-Infection with Mycobacterium tuberculosis Impairs HIV-Specific CD8+ and CD4+ T Cell Functionality
Chetty, Shivan; Govender, Pamla; Zupkosky, Jennifer; Pillay, Mona; Ghebremichael, Musie; Moosa, Mahomed-Yunus S.; Ndung’u, Thumbi; Porichis, Filippos; Kasprowicz, Victoria O.; Paxton, William A. (Public Library of Science, 2015)The ability of antigen-specific T cells to simultaneously produce multiple cytokines is thought to correlate with the functional capacity and efficacy of T cells. These 'polyfunctional' T cells have been associated with ... -
High throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometry
Porichis, Filippos; Hart, Meghan G.; Griesbeck, Morgane; Everett, Holly L.; Hassan, Muska; Baxter, Amy E.; Lindqvist, Madelene; Miller, Sara M.; Soghoian, Damien Z.; Kavanagh, Daniel G.; Reynolds, Susan; Norris, Brett; Mordecai, Scott K.; Nguyen, Quan; Lai, Chunfai; Kaufmann, Daniel E. (2014)Fluorescent in situ hybridization (FISH) is a method that uses fluorescent probes to detect specific nucleic acid sequences at the single cell level. Here we describe optimized protocols that exploit a highly sensitive ... -
HIV-1 Antibody Neutralization Breadth Is Associated with Enhanced HIV-Specific CD4+ T Cell Responses
Ranasinghe, Srinika; Soghoian, Damien; Lindqvist, Madelene; Ghebremichael, Musie; Donaghey, Faith; Carrington, Mary; Seaman, Michael; Kaufmann, Daniel; Walker, Bruce; Porichis, Filippos (American Society for Microbiology, 2016)Antigen-specific CD4(+) T helper cell responses have long been recognized to be a critical component of effective vaccine immunity. CD4(+) T cells are necessary to generate and maintain humoral immune responses by providing ...