Browsing by Author "Rios, Xavier"

Now showing items 1-4 of 4

    • Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems 

      DiCarlo, James; Norville, Julie; Mali, Prashant; Rios Villanueva, Xavier; Aach, John Dennis; Church, George McDonald (Oxford University Press, 2013)
      Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity to provide adaptive immunity against invading foreign nucleic ...

    • Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers 

      Briggs, Adrian; Rios, Xavier; Chari, Rajagopal; Yang, Luhan; Zhang, Feng; Mali, Prashant; Church, George McDonald (Oxford University Press, 2012)
      DNA built from modular repeats presents a challenge for gene synthesis. We present a solid surface-based sequential ligation approach, which we refer to as iterative capped assembly (ICA), that adds DNA repeat monomers ...

    • Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases 

      Rios, Xavier; Briggs, Adrian; Christodoulou, Danos C.; Gorham, Josh McClean; Seidman, Jonathan G.; Church, George McDonald (Public Library of Science, 2012)
      Recent advances allow multiplexed genome engineering in E. coli, employing easily designed oligonucleotides to edit multiple loci simultaneously. A similar technology in human cells would greatly expedite functional genomics, ...

    • Toward Multiplex Genome Engineering in Mammalian Cells 

      Rios Villanueva, Xavier (2013-10-18)
      Given the explosion in human genetic data, new high-throughput genetic methods are necessary for studying variants and elucidating their role in human disease. In Chapter I, I will expand on this concept and describe ...