Now showing items 1-5 of 5

    • Bacteriophage T7 DNA polymerase – sequenase 

      Zhu, Bin (Frontiers Media S.A., 2014)
      An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; ...
    • Flap endonuclease of bacteriophage T7: Possible roles in RNA primer removal, recombination and host DNA breakdown 

      Mitsunobu, Hitoshi; Zhu, Bin; Lee, Seung-Joo; Tabor, Stanley; Richardson, Charles C (Landes Bioscience, 2014)
      Gene 6 protein of bacteriophage T7 has 5′-3′-exonuclease activity specific for duplex DNA. We have found that gene 6 protein also has flap endonuclease activity. The flap endonuclease activity is considerably weaker than ...
    • Mechanism of Sequence-Specific Template Binding by the DNA Primase of Bacteriophage T7 

      Lee, Seung-Joo; Zhu, Bin; Hamdan, Samir M.; Richardson, Charles C. (Oxford University Press, 2010)
      DNA primases catalyze the synthesis of the oligoribonucleotides required for the initiation of lagging strand DNA synthesis. Biochemical studies have elucidated the mechanism for the sequence-specific synthesis of primers. ...
    • Syn5 RNA polymerase synthesizes precise run-off RNA products 

      Zhu, Bin; Tabor, Stanley; Richardson, Charles C. (Oxford University Press, 2013)
      The enzyme predominantly used for in vitro run-off RNA synthesis is bacteriophage T7 RNA polymerase. T7 RNA polymerase synthesizes, in addition to run-off products of precise length, transcripts with an additional ...
    • Synthesis of 2′-Fluoro RNA by Syn5 RNA polymerase 

      Zhu, Bin; Hernandez, Alfredo; Tan, Min; Wollenhaupt, Jan; Tabor, Stanley; Richardson, Charles C. (Oxford University Press, 2015)
      The substitution of 2′-fluoro for 2′-hydroxyl moieties in RNA substantially improves the stability of RNA. RNA stability is a major issue in RNA research and applications involving RNA. We report that the RNA polymerase ...