Now showing items 1-20 of 30

    • Analysis of rapidly synthesized guest-filled porous complexes with synchrotron radiation: practical guidelines for the crystalline sponge method 

      Ramadhar, Timothy R.; Zheng, Shao-Liang; Chen, Yu-Sheng; Clardy, Jon (International Union of Crystallography, 2015)
      A detailed set of synthetic and crystallographic guidelines for the crystalline sponge method based upon the analysis of expediently synthesized crystal sponges using third-generation synchrotron radiation are reported. ...
    • Antibiotic and Antimalarial Quinones from Fungus-Growing Ant-Associated Pseudonocardia sp. 

      Carr, Gavin; Derbyshire, Emily; Caldera, Eric; Currie, Cameron R.; Clardy, Jon C. (American ChemicalSociety and AmericanSociety of Pharmacognosy, 2012)
      Three new members of the angucycline class of antibiotics, pseudonocardones A–C (1–3), along with the known antibiotics 6-deoxy-8-O-methylrabelomycin (4) and X-14881 E (5) have been isolated from the culture of a Pseudonocardia ...
    • A bacterial sulfonolipid triggers multicellular development in the closest living relatives of animals 

      Alegado, Rosanna A; Brown, Laura W; Cao, Shugeng; Dermenjian, Renee K; Zuzow, Richard; Fairclough, Stephen R; Clardy, Jon C.; King, Nicole (eLife Sciences Publications, Ltd, 2012)
      Bacterially-produced small molecules exert profound influences on animal health, morphogenesis, and evolution through poorly understood mechanisms. In one of the closest living relatives of animals, the choanoflagellate ...
    • Bactobolin Resistance Is Conferred by Mutations in the L2 Ribosomal Protein 

      Chandler, Josephine R.; Truong, Thao T.; Silva, Patricia M.; Seyedsayamdost, Mohammad; Carr, Gavin; Radey, Matthew; Jacobs, Michael A.; Sims, Elizabeth H.; Clardy, Jon C.; Greenberg, E. Peter (American Society of Microbiology, 2012)
      Burkholderia thailandensis produces a family of polyketide-peptide molecules called bactobolins, some of which are potent antibiotics. We found that growth of B. thailandensis at 30°C versus that at 37°C resulted in increased ...
    • Catecholate Siderophores Protect Bacteria from Pyochelin Toxicity 

      Adler, Conrado; Corbalán, Natalia S.; Seyedsayamdost, Mohammad; Pomares, María Fernanda; de Cristóbal, Ricardo E.; Clardy, Jon C.; Kolter, Roberto Guillermo; Vincent, Paula A. (Public Library of Science, 2012)
      Background: Bacteria produce small molecule iron chelators, known as siderophores, to facilitate the acquisition of iron from the environment. The synthesis of more than one siderophore and the production of multiple ...
    • Discovery and Characterization of the Cylindrocyclophane Biosynthetic Pathway 

      Nakamura, Hitomi (2016-05-17)
      Nature constructs structurally diverse, bioactive molecules using enzymes. Many enzymes catalyze synthetically challenging reactions under mild, physiological conditions. Consequently, they have long been a source of ...
    • Discovery of 3-Formyl-Tyrosine Metabolites from Pseudoalteromonas tunicata through Heterologous Expression 

      Blasiak, Leah C.; Clardy, Jon C. (American Chemical Society, 2009)
      Genome mining and identification of natural product gene clusters typically relies on the presence of canonical nonribosomal polypeptide synthetase (NRPS) or polyketide synthase (PKS) domains. Recently, other condensation ...
    • Draft Genome Sequence of Shewanella sp. Strain P1-14-1, a Bacterial Inducer of Settlement and Morphogenesis in Larvae of the Marine Hydroid Hydractinia echinata 

      Rischer, Maja; Klassen, Jonathan L.; Wolf, Thomas; Guo, Huijuan; Shelest, Ekaterina; Clardy, Jon; Beemelmanns, Christine (American Society for Microbiology, 2016)
      The assembly and annotation of the draft genome sequence of Shewanella sp. strain P1-14-1 are reported here to investigate the genes responsible for interkingdom interactions, secondary metabolite production, and microbial ...
    • Draft Genome Sequences of Six Pseudoalteromonas Strains, P1-7a, P1-9, P1-13-1a, P1-16-1b, P1-25, and P1-26, Which Induce Larval Settlement and Metamorphosis in Hydractinia echinata 

      Klassen, Jonathan L.; Wolf, Thomas; Rischer, Maja; Guo, Huijuan; Shelest, Ekaterina; Clardy, Jon; Beemelmanns, Christine (American Society for Microbiology, 2015)
      To gain a broader understanding of the importance of a surface-associated lifestyle and morphogenic capability, we have assembled and annotated the genome sequences of Pseudoalteromonas strains P1-7a, P1-9, P1-13-1a, ...
    • Genome Sequence of Streptomyces sp. Strain RTd22, an Endophyte of the Mexican Sunflower 

      Chagas, Fernanda O.; Ruzzini, Antonio C.; Bacha, Larissa V.; Samborskyy, Markyian; Conti, Raphael; Pessotti, Rita C.; de Oliveira, Luciana G.; Clardy, Jon; Pupo, Monica T. (American Society for Microbiology, 2016)
      We report here the complete genome sequence of Streptomyces sp. strain RTd22, an endophytic actinobacterium that was isolated from the roots of the Mexican sunflower Tithonia diversifolia. The bacterium’s 11.1-Mb linear ...
    • Genome Sequences of Three Pseudoalteromonas Strains (P1-8, P1-11, and P1-30), Isolated from the Marine Hydroid Hydractinia echinata 

      Klassen, Jonathan L.; Rischer, Maja; Wolf, Thomas; Guo, Huijuan; Shelest, Ekaterina; Clardy, Jon; Beemelmanns, Christine (American Society for Microbiology, 2015)
      The genomes of three Pseudoalteromonas strains (P1-8, P1-11, and P1-30) were sequenced and assembled. These genomes will inform future study of the genes responsible for the production of biologically active compounds ...
    • A High-Throughput Screen Identifies a New Natural Product with Broad-Spectrum Antibacterial Activity 

      Ymele-Leki, Patrick; Cao, Shugeng; Sharp, Jared D'Alonzo; Lambert, Kathleen G; McAdam, Alexander John; Husson, Robert N; Tamayo, Giselle; Clardy, Jon C.; Watnick, Paula Ivonne (Public Library of Science, 2012)
      Due to the inexorable invasion of our hospitals and communities by drug-resistant bacteria, there is a pressing need for novel antibacterial agents. Here we report the development of a sensitive and robust but low-tech and ...
    • Hybrid Biosynthesis of Roseobacticides from Algal and Bacterial Precursor Molecules 

      Seyedsayamdost, Mohammad R.; Wang, Rurun; Kolter, Roberto; Clardy, Jon (American Chemical Society, 2014)
      Roseobacticides regulate the symbiotic relationship between a marine bacterium (Phaeobacter inhibens) and a marine microalga (Emiliania huxleyi). This relationship can be mutualistic, when the algal host provides food for ...
    • Identification and Characterization of Small Molecule Inhibitors of a Class I Histone Deacetylase from Plasmodium falciparum 

      Nguyen, Cokey; Urgaonkar, Sameer; Cortese, Joseph; Barker, Robert H.; Greenberg, Edward; Tang, Weiping; Patel, Vishal; Mazitschek, Ralph; Coleman, Bradley Ian; Bradner, James Elliott; Schreiber, Stuart L.; Duraisingh, Manoj T.; Wirth, Dyann Fergus; Clardy, Jon C. (American Chemical Society, 2009)
      A library of approximately 2000 small molecules biased toward inhibition of histone deacetylases was assayed for antimalarial activity in a high-throughput P. falciparum viability assay. Active compounds were cross-analyzed ...
    • Identification of Anziaic Acid, a Lichen Depside from Hypotrachyna sp., as a New Topoisomerase Poison Inhibitor 

      Cheng, Bokun; Cao, Shugeng; Vasquez, Victor; Annamalai, Thirunavukkarasu; Tamayo-Castillo, Giselle; Clardy, Jon C.; Tse-Dinh, Yuk-Ching (Public Library of Science, 2013)
      Topoisomerase inhibitors are effective for antibacterial and anticancer therapy because they can lead to the accumulation of the intermediate DNA cleavage complex formed by the topoisomerase enzymes, which trigger cell ...
    • Isolation and Synthesis of a Bacterially Produced Inhibitor of Rosette Development in Choanoflagellates 

      Cantley, Alexandra M.; Woznica, Arielle; Beemelmanns, Christine; King, Nicole; Clardy, Jon (American Chemical Society, 2016)
      The choanoflagellate Salpingoeca rosetta is a microbial marine eukaryote that can switch between unicellular and multicellular states. As one of the closest living relatives of animals, this organism has become a model for ...
    • Microtermolides A and B from Termite-Associated Streptomyces sp. and Structural Revision of Vinylamycin 

      Carr, Gavin; Poulsen, Michael; Klassen, Jonathan L.; Hou, Yanpeng; Wyche, Thomas P.; Bugni, Tim S.; Currie, Cameron R.; Clardy, Jon C. (American Chemical Society, 2012)
      Microtermolides A (1) and B (2) were isolated from a Streptomyces sp. strain associated with fungus-growing termites. The structures of 1 and 2 were determined by 1D- and 2D-NMR spectroscopy and high-resolution mass ...
    • Minimum Information about a Biosynthetic Gene cluster 

      Medema, Marnix H; Kottmann, Renzo; Yilmaz, Pelin; Cummings, Matthew; Biggins, John B; Blin, Kai; de Bruijn, Irene; Chooi, Yit Heng; Claesen, Jan; Coates, R Cameron; Cruz-Morales, Pablo; Duddela, Srikanth; Düsterhus, Stephanie; Edwards, Daniel J; Fewer, David P; Garg, Neha; Geiger, Christoph; Gomez-Escribano, Juan Pablo; Greule, Anja; Hadjithomas, Michalis; Haines, Anthony S; Helfrich, Eric J N; Hillwig, Matthew L; Ishida, Keishi; Jones, Adam C; Jones, Carla S; Jungmann, Katrin; Kegler, Carsten; Kim, Hyun Uk; Kötter, Peter; Krug, Daniel; Masschelein, Joleen; Melnik, Alexey V; Mantovani, Simone M; Monroe, Emily A; Moore, Marcus; Moss, Nathan; Nützmann, Hans-Wilhelm; Pan, Guohui; Pati, Amrita; Petras, Daniel; Reen, F Jerry; Rosconi, Federico; Rui, Zhe; Tian, Zhenhua; Tobias, Nicholas J; Tsunematsu, Yuta; Wiemann, Philipp; Wyckoff, Elizabeth; Yan, Xiaohui; Yim, Grace; Yu, Fengan; Xie, Yunchang; Aigle, Bertrand; Apel, Alexander K; Balibar, Carl J; Balskus, Emily Patricia; Barona-Gómez, Francisco; Bechthold, Andreas; Bode, Helge B; Borriss, Rainer; Brady, Sean F; Brakhage, Axel A; Caffrey, Patrick; Cheng, Yi-Qiang; Clardy, Jon C.; Cox, Russell J; De Mot, René; Donadio, Stefano; Donia, Mohamed S; van der Donk, Wilfred A; Dorrestein, Pieter C; Doyle, Sean; Driessen, Arnold J M; Ehling-Schulz, Monika; Entian, Karl-Dieter; Fischbach, Michael A; Gerwick, Lena; Gerwick, William H; Gross, Harald; Gust, Bertolt; Hertweck, Christian; Höfte, Monica; Jensen, Susan E; Ju, Jianhua; Katz, Leonard; Kaysser, Leonard; Klassen, Jonathan L; Keller, Nancy P; Kormanec, Jan; Kuipers, Oscar P; Kuzuyama, Tomohisa; Kyrpides, Nikos C; Kwon, Hyung-Jin; Lautru, Sylvie; Lavigne, Rob; Lee, Chia Y; Linquan, Bai; Liu, Xinyu; Liu, Wen; Luzhetskyy, Andriy; Mahmud, Taifo; Mast, Yvonne; Méndez, Carmen; Metsä-Ketelä, Mikko; Micklefield, Jason; Mitchell, Douglas A; Moore, Bradley S; Moreira, Leonilde M; Müller, Rolf; Neilan, Brett A; Nett, Markus; Nielsen, Jens; O, Fergal; Oikawa, Hideaki; Osbourn, Anne; Osburne, Marcia S; Ostash, Bohdan; Payne, Shelley M; Pernodet, Jean-Luc; Petricek, Miroslav; Piel, Jörn; Ploux, Olivier; Raaijmakers, Jos M; Salas, José A; Schmitt, Esther K; Scott, Barry; Seipke, Ryan F; Shen, Ben; Sherman, David; Sivonen, Kaarina; Smanski, Michael J; Sosio, Margherita; Stegmann, Evi; Süssmuth, Roderich D; Tahlan, Kapil; Thomas, Christopher M; Tang, Yi; Truman, Andrew W; Viaud, Muriel; Walton, Jonathan D; Walsh, Christopher T.; Weber, Tilmann; van Wezel, Gilles P; Wilkinson, Barrie; Willey, Joanne M; Wohlleben, Wolfgang; Wright, Gerard D; Ziemert, Nadine; Zhang, Changsheng; Zotchev, Sergey B; Breitling, Rainer; Takano, Eriko; Glöckner, Frank Oliver (Springer Nature, 2015)
      A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded in biosynthetic gene clusters. Information about these clusters, pathways and metabolites is ...
    • Mixing and Matching Siderophore Clusters: Structure and Biosynthesis of Serratiochelins from Serratia sp. V4 

      Seyedsayamdost, Mohammad; Cleto, Sara; Carr, Gavin; Vlamakis, Hera C.; João Vieira, Maria; Kolter, Roberto Guillermo; Clardy, Jon C. (American Chemical Society, 2012)
      Interrogation of the evolutionary history underlying the remarkable structures and biological activities of natural products has been complicated by not knowing the functions they have evolved to fulfill. Siderophores—soluble, ...
    • Natural Product Screening Reveals Naphthoquinone Complex I Bypass Factors 

      Vafai, Scott B.; Mevers, Emily; Higgins, Kathleen W.; Fomina, Yevgenia; Zhang, Jianming; Mandinova, Anna; Newman, David; Shaw, Stanley Y.; Clardy, Jon; Mootha, Vamsi K. (Public Library of Science, 2016)
      Deficiency of mitochondrial complex I is encountered in both rare and common diseases, but we have limited therapeutic options to treat this lesion to the oxidative phosphorylation system (OXPHOS). Idebenone and menadione ...