Now showing items 1-11 of 11

    • Fluctuation Timescales in Bacterial Gene Expression 

      Lord, Nathan Dale (2014-02-25)
      The stochastic nature of intracellular chemistry guarantees that even genetically identical cells sharing an environment will differ in composition. The question of whether this chemical diversity translates into significant ...
    • Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells 

      Okumus, Burak; Landgraf, Dirk; Lai, Ghee Chuan; Bakhsi, Somenath; Arias-Castro, Juan Carlos; Yildiz, Sadik; Huh, Dann; Fernandez-Lopez, Raul; Peterson, Celeste N.; Toprak, Erdal; El Karoui, Meriem; Paulsson, Johan (Nature Publishing Group, 2016)
      Many key regulatory proteins in bacteria are present in too low numbers to be detected with conventional methods, which poses a particular challenge for single-cell analyses because such proteins can contribute greatly to ...
    • Memory and Modularity in Cell-Fate Decision Making 

      Norman, Thomas M.; Lord, Nathan D.; Paulsson, Johan; Losick, Richard (2014)
      Genetically identical cells sharing an environment can display markedly different phenotypes. It is often unclear how much of this variation derives from chance, external signals, or attempts by individual cells to exert ...
    • Memory in a phenotypic switch and noise in gene networks 

      Norman, Thomas Maxwell (2014-02-25)
      Many cell types stochastically switch phenotypes under some conditions, so that genetically identical sister cells may behave quite differently in a common environment. This non-genetic variability likely arises from noise ...
    • Nanoscale Organization and Optical Observation of Biomolecules With DNA Nanotechnology 

      Dai, Mingjie (2016-05-19)
      Understanding biomolecular information at the single-molecule level requires tools for manipulating and observing individual biomolecules at the nanoscale. Programmable DNA nanotechnology provides an ideal interface to ...
    • Promoter Decoding of Transcription Factor Translocation Dynamics 

      Hansen, Anders Sejr (2015-05-11)
      Many cellular signaling pathways exhibit a bowtie topology: multiple distinct signal inputs converge on a single master transcription factor, which controls the expression of downstream genes. Recent evidence suggests that ...
    • Quantifying Localizations and Dynamics in Single Bacterial Cells 

      Landgraf, Dirk (2012-11-19)
      Levels of macromolecules fluctuate both spatially and temporally in individual cells. Such heterogeneity could be exploited for bet hedging in uncertain environments, or be suppressed by negative feedback if perturbations ...
    • Random Timing in Signaling Cascades 

      Pedraza, Juan M; Paulsson, Johan Martin (European Molecular Biology Organization, 2007)
    • Segregation of molecules at cell division reveals native protein localization 

      Landgraf, Dirk; Okumus, Burak; Chien, Peter; Baker, Tania A.; Paulsson, Johan (2013)
      We introduce a non-intrusive method exploiting post-division single-cell variability to validate protein localization. The results show that Clp proteases, widely reported to form biologically relevant foci, are in fact ...
    • Super Resolution Imaging by Programmable Autonomous Blinking 

      Avendaño Amado, Maier S. (2015-05-18)
      Current far-field super-resolution techniques offer unprecedented spatial resolution, however, the identification and quantification of multiple molecules that cannot be spatially resolved remains challenging, mainly ...
    • Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag 

      Ke, Na; Landgraf, Dirk; Paulsson, Johan; Berkmen, Mehmet (American Society for Microbiology, 2016)
      ABSTRACT The use of fluorescent and luminescent proteins in visualizing proteins has become a powerful tool in understanding molecular and cellular processes within living organisms. This success has resulted in an ...