Browsing by Author "Hung, Deborah"

Now showing items 1-14 of 14

    • Engineering Escherichia Coli Fatty Acid Metabolism for the Production of Biofuel Precursors 

      Ford, Tyler John (2015-05-07)
      Medium chain fatty acids (MCFAs, 6-12 carbons) are potential precursors to biofuels with properties similar to gasoline and diesel fuel but are not native products of Escherichia coli fatty acid synthesis. Herein we engineer ...

    • Genomic epidemiology of the Escherichia coli O104:H4 outbreaks in Europe, 2011 

      Grad, Yonatan Hagai; Lipsitch, Marc; Feldgarden, M.; Arachchi, H. M.; Cerqueira, G. C.; Fitzgerald, Michael L.; Godfrey, P.; Haas, Brandon Russell; Murphy, C. I.; Russ, C.; Sykes, Sean; Walker, B. J.; Wortman, J. R.; Young, Sarah; Zeng, Q.; Abouelleil, A.; Bochicchio, J.; Chauvin, S.; DeSmet, T.; Gujja, S.; Mccowan, Caryn Alissa; Montmayeur, A.; Steelman, S.; Frimodt-Moller, J.; Petersen, A. M.; Struve, C.; Krogfelt, K. A.; Bingen, E.; Weill, F.-X.; Lander, Eric Steven; Nusbaum, C.; Birren, B. W.; Hung, Deborah Tan; Hanage, William P. (Proceedings of the National Academy of Sciences, 2012)
      The degree to which molecular epidemiology reveals information about the sources and transmission patterns of an outbreak depends on the resolution of the technology used and the samples studied. Isolates of Escherichia ...

    • A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes 

      Avraham, Roi; Haseley, Nathan; Fan, Amy; Bloom-Ackermann, Zohar; Livny, Jonathan; Hung, Deborah Tan (Springer Nature, 2016)
      The ability to simultaneously characterize the bacterial and host expression programs during infection would facilitate a comprehensive understanding of pathogen-host interactions. While RNA-Seq has greatly advanced our ...

    • Identification of Host Factors Required for Anthrax Lethal Toxin Intoxication Using Chemical Genetic and RNAi Approaches 

      Slater, Louise (2013-02-11)
      Bacterial toxins have co-opted host cell machinery in order to enter cells and exert their deleterious effects. Anthrax toxin is composed of the receptor binding protein protective antigen (PA), and the enzymatic subunits ...

    • Identification of Host-Targeted Small Molecules That Restrict Intracellular Mycobacterium tuberculosis Growth 

      Stanley, Sarah A.; Barczak, Amy K.; Silvis, Melanie R.; Luo, Samantha S.; Sogi, Kimberly; Vokes, Martha; Bray, Mark-Anthony; Carpenter, Anne E.; Moore, Christopher B.; Siddiqi, Noman; Rubin, Eric J.; Hung, Deborah T. (Public Library of Science, 2014)
      Mycobacterium tuberculosis remains a significant threat to global health. Macrophages are the host cell for M. tuberculosis infection, and although bacteria are able to replicate intracellularly under certain conditions, ...

    • An Immune Cell Signature of Bacterial Sepsis 

      Reyes, Miguel; Filbin, Michael; Bhattacharyya, Roby; Billman, Kianna; Eisenhaure, Thomas; Hung, Deborah; Levy, Bruce; Baron, Rebecca; Blainey, Paul; Goldberg, Marcia; Hacohen, Nir (Springer Science and Business Media LLC, 2020-02-17)
      Dysregulation of the immune response to bacterial infection can lead to sepsis, a condition with high mortality. Multiple whole-blood gene expression studies have defined sepsis-associated molecular signatures but did not ...

    • Independent Large Scale Duplications in Multiple M. tuberculosis Lineages Overlapping the Same Genomic Region 

      Weiner, Brian; Victor, Thomas C.; Warren, Robert M.; Plikaytis, Bonnie B.; Posey, James E.; van Helden, Paul D.; Gey van Pittius, Nicolass C.; Koehrsen, Michael; Sisk, Peter; Stolte, Christian; White, Jared; Gagneux, Sebastien; Birren, Bruce; Gomez, James E.; Sloutsky, Alexander; Hung, Deborah Tan; Murray, Megan Blanche; Galagan, James E (Public Library of Science, 2012)
      Mycobacterium tuberculosis, the causative agent of most human tuberculosis, infects one third of the world's population and kills an estimated 1.7 million people a year. With the world-wide emergence of drug resistance, ...

    • Large-Scale Chemical–genetics Yields New M. Tuberculosis Inhibitor Classes 

      LaVerriere, Emily; Meyer, Elisabeth; Kawate, Tomohiko; Gomez, James; Gardner, Michelle; Cigarroa Kennedy, Sofia; Wakabayashi, Shoko; Watson, Christopher; Fitzgerald, Michael; Johnson, Eachan; Office, Emma; Stanley, Mary; Audette, Rebecca; Bandyopadhyay, Nirmalya; Betancourt, Natalia; Delano, Kayla; Da Silva, Israel; Davis, Joshua; Gallo, Christina; Golas, Aaron; Guinn, Kristine; Korn, Rebecca; McConnell, Jennifer; Moss, Caitlin; Murphy, Kenan; Nietupski, Raymond; Papavinasasundaram, Kadamba; Pinkham, Jessica; Pino, Paula; Proulx, Megan; Ruecker, Nadine; Song, Naomi; Thompson, Matthew; Trujillo, Carolina; Metcalf-Wallach, Joshua; Ioerger, Thomas; Lander, Eric; Hubbard, Brian; Serrano-Wu, Michael; Ehrt, Sabine; Rubin, Eric; Sassetti, Christopher; Schnappinger, Dirk; Hung, Deborah (Springer Science and Business Media LLC, 2019-06-19)
      New antibiotics are needed to combat rising resistance, with new Mycobacterium tuberculosis (Mtb) drugs of highest priority. Conventional whole-cell and biochemical antibiotic screens have failed. We developed a novel ...

    • MetaMerge: scaling up genome-scale metabolic reconstructions with application to Mycobacterium tuberculosis 

      Chindelevitch, Leonid; Stanley, Sarah; Hung, Deborah; Regev, Aviv; Berger, Bonnie (BioMed Central, 2012)
      Reconstructed models of metabolic networks are widely used for studying metabolism in various organisms. Many different reconstructions of the same organism often exist concurrently, forcing researchers to choose one of ...

    • Molecular Genetics of Beta-Lactam Sensitivity and Resistance in Mycobacterium Tuberculosis 

      Wivagg, Carl (2012-10-25)
      Mycobacterium tuberculosis threatens global health. Its thick, impermeable cell wall renders it tolerant to high doses of many antibiotics. While we understand the biochemical functions of many cell wall-modifying enzymes, ...

    • Persistent bacterial infections, antibiotic tolerance, and the oxidative stress response 

      Grant, Sarah Schmidt; Hung, Deborah T. (Landes Bioscience, 2013)
      Certain bacterial pathogens are able to evade the host immune system and persist within the human host. The consequences of persistent bacterial infections potentially include increased morbidity and mortality from the ...

    • Pseudomonas aeruginosa Infection of Zebrafish Involves both Host and Pathogen Determinants 

      Clatworthy, Anne; Lee, Jenny See-Wai; Leibman, Mark; Kostun, Zachary; Davidson, Alan; Hung, Deborah (American Society for Microbiology, 2009)
      Zebrafish (Danio rerio) have a number of strengths as a host model for infection, including genetic tractability, a vertebrate immune system similar to that of mammals, ease and scale of laboratory handling, which allows ...

    • Sensitive, Specific Polymorphism Discovery in Bacteria Using Massively Parallel Sequencing 

      Nusbaum, Chad; Ohsumi, Toshiro K.; Gomez, James; Aquadro, John; Victor, Thomas C.; Warren, Robert M.; Hung, Deborah; Birren, Bruce W.; Lander, Eric; Jaffe, David B. (Nature Publishing Group, 2008)
      Our variant ascertainment algorithm, VAAL, uses massively parallel DNA sequence data to identify differences between bacterial genomes with high sensitivity and specificity. VAAL detected B98% of differences (including ...

    • The Two-Component Sensor KinB Regulates Pseudomonas aeruginosa Virulence 

      Chand, Nikhilesh (2013-02-22)
      Bacteria commonly use two-component sensors to sense and respond to their environment. The Gram-negative opportunistic pathogen Pseudomonas aeruginosa has one of the largest sets of two-component sensors known in bacteria, ...