Browsing by Author "Makrigiorgos, Gerassimos"
Now showing items 1-11 of 11
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COLD-PCR Amplification of Bisulfite-Converted DNA Allows the Enrichment and Sequencing of Rare Un-Methylated Genomic Regions
Castellanos-Rizaldos, Elena; Milbury, Coren A.; Karatza, Elli; Chen, Clark C.; Makrigiorgos, G. Mike; Merewood, Anne (Public Library of Science, 2014)Aberrant hypo-methylation of DNA is evident in a range of human diseases including cancer and diabetes. Development of sensitive assays capable of detecting traces of un-methylated DNA within methylated samples can be ... -
A Comparison of RNA Amplification Techniques at Sub-Nanogram Input Concentration
Lang, Julie E; Magbanua, Mark Jesus M; Scott, Janet H; Makrigiorgos, Gerassimos M.; Wang, Gang; Federman, Scot; Esserman, Laura J; Park, John W.; Haqq, Christopher M (BioMed Central, 2009)Background: Gene expression profiling of small numbers of cells requires high-fidelity amplification of sub-nanogram amounts of RNA. Several methods for RNA amplification are available; however, there has been little ... -
Differential strand separation at critical temperature: A minimally disruptive enrichment method for low-abundance unknown DNA mutations
Guha, Minakshi; Castellanos-Rizaldos, Elena; Liu, Pingfang; Mamon, Harvey Jay; Makrigiorgos, Gerassimos M. (Oxford University Press, 2012)Detection of low-level DNA variations in the presence of wild-type DNA is important in several fields of medicine, including cancer, prenatal diagnosis and infectious diseases. PCR-based methods to enrich mutations during ... -
DISSECT Method Using PNA-LNA Clamp Improves Detection of EGFR T790m Mutation
Guha, Minakshi; Castellanos-Rizaldos, Elena; Makrigiorgos, G. Mike (Public Library of Science, 2013)Non-small cell lung cancer (NSCLC) patients treated with small molecule EGFR inhibitors, such as gefitinib, frequently develop drug resistance due to the presence of secondary mutations like the T790M mutation on EGFR exon ... -
Elimination of unaltered DNA in mixed clinical samples via nuclease-assisted minor-allele enrichment
Song, Chen; Liu, Yibin; Fontana, Rachel; Makrigiorgos, Alexander; Mamon, Harvey; Kulke, Matthew H.; Makrigiorgos, G. Mike (Oxford University Press, 2016)Presence of excess unaltered, wild-type (WT) DNA providing no information of biological or clinical value often masks rare alterations containing diagnostic or therapeutic clues in cancer, prenatal diagnosis, infectious ... -
Enrichment of Mutations in Multiple DNA Sequences Using COLD-PCR in Emulsion
Castellanos-Rizaldos, Elena; Milbury, Coren Audrey; Makrigiorgos, G. Mike (Public Library of Science, 2012)Background: Multiplex detection of low-level mutant alleles in the presence of wild-type DNA would be useful for several fields of medicine including cancer, pre-natal diagnosis and infectious diseases. COLD-PCR is a ... -
FLAG Assay as a Novel Method for Real-Time Signal Generation During PCR: Application to Detection and Genotyping of KRAS Codon 12 Mutations
Amicarelli, Giulia; Shehi, Erlet; Makrigiorgos, Gerassimos M.; Adlerstein, Daniel (Oxford University Press, 2007)Real-time signal generation methods for detection and characterization of low-abundance mutations in genomic DNA are powerful tools for cancer diagnosis and prognosis. Mutations in codon 12 of the oncogene KRAS, for example, ... -
Ice-COLD-PCR Enables Rapid Amplification and Robust Enrichment for Low-Abundance Unknown DNA Mutations
Milbury, Coren Audrey; Li, Jin; Makrigiorgos, Gerassimos M. (Oxford University Press, 2010)Identifying low-abundance mutations within wild-type DNA is important in several fields of medicine, including cancer, prenatal diagnosis and infectious diseases. However, utilizing the clinical and diagnostic potential ... -
Image-guided radiotherapy platform using single nodule conditional lung cancer mouse models
Herter-Sprie, Grit S.; Korideck, Houari; Christensen, Camilla L.; Herter, Jan M.; Rhee, Kevin; Berbeco, Ross I.; Bennett, David G.; Akbay, Esra A.; Kozono, David; Mak, Raymond H.; Makrigiorgos, G. Mike; Kimmelman, Alec C.; Wong, Kwok-Kin (2014)Close resemblance of murine and human trials is essential to achieve the best predictive value of animal-based translational cancer research. Kras-driven genetically engineered mouse models of non-small cell lung cancer ... -
Metastasis-associated MCL1 and P16 copy number alterations dictate resistance to vemurafenib in a BRAFV600E patient-derived papillary thyroid carcinoma preclinical model
Duquette, Mark; Sadow, Peter M.; Husain, Amjad; Sims, Jennifer N.; Antonello, Zeus A.; Fischer, Andrew H.; Song, Chen; Castellanos-Rizaldos, Elena; Makrigiorgos, G. Mike; Kurebayashi, Junichi; Nose, Vania; Van Hummelen, Paul; Bronson, Roderick T.; Vinco, Michelle; Giordano, Thomas J.; Dias-Santagata, Dora; Pandolfi, Pier Paolo; Nucera, Carmelo (Impact Journals LLC, 2015)BRAFV600E mutation exerts an essential oncogenic function in many tumors, including papillary thyroid carcinoma (PTC). Although BRAFV600E inhibitors are available, lack of response has been frequently observed. To study ... -
Nanoparticle Mediated Tumor Vascular Disruption: A Novel Strategy in Radiation Therapy
Kunjachan, Sijumon; Detappe, Alexandre; Kumar, Rajiv; Ireland, Thomas; Cameron, Lisa; Biancur, Douglas E.; Motto-Ros, Vincent; Sancey, Lucie; Sridhar, Srinivas; Makrigiorgos, Gerassimos M.; Berbeco, Ross I. (American Chemical Society (ACS), 2015)More than 50% of all cancer patients receive radiation therapy. The clinical delivery of curative radiation dose is strictly restricted by the proximal healthy tissues. We propose a dual-targeting strategy using ...