Now showing items 1-11 of 11

    • Coordination of Individual and Ensemble Cytoskeletal Motors Studied Using Tools from DNA Nanotechnology 

      Derr, Nathan Dickson (2013-09-30)
      The cytoskeletal molecular motors kinesin-1 and cytoplasmic dynein drive many diverse functions within eukaryotic cells. They are responsible for numerous spatially and temporally dependent intracellular processes crucial ...
    • DNA Brick Crystals with Prescribed Depth 

      Ke, Yonggang; Ong, Luvena L.; Sun, Wei; Song, Jie; Dong, Mingdong; Shih, William M.; Yin, Peng (2014)
      We describe a general framework for constructing two-dimensional crystals with prescribed depth and sophisticated three-dimensional features. These crystals may serve as scaffolds for the precise spatial arrangements of ...
    • DNA Origami Nanoparticles for Cell Delivery: The Effect of Shape and Surface Functionalization on Cell Internalization 

      Graf, Franziska (2012-09-14)
      An outstanding challenge in modern medicine is the safe and efficient delivery of drugs. One approach to improve drug delivery yield and increase specificity towards diseased cells, is to employ a drug carrier to facilitate ...
    • Membrane-Assisted Growth of DNA Origami Nanostructure Arrays 

      Kocabey, Samet; Kempter, Susanne; List, Jonathan; Xing, Yongzheng; Bae, Wooli; Schiffels, Daniel; Shih, William M.; Simmel, Friedrich C.; Liedl, Tim (American Chemical Society, 2015)
      Biological membranes fulfill many important tasks within living organisms. In addition to separating cellular volumes, membranes confine the space available to membrane-associated proteins to two dimensions (2D), which ...
    • Nanoscale Organization and Optical Observation of Biomolecules With DNA Nanotechnology 

      Dai, Mingjie (2016-05-19)
      Understanding biomolecular information at the single-molecule level requires tools for manipulating and observing individual biomolecules at the nanoscale. Programmable DNA nanotechnology provides an ideal interface to ...
    • Rapid Prototyping of 3D DNA-Origami Shapes with caDNAno 

      Douglas, Shawn Michael; Marblestone, Adam H.; Teerapittayanon, Surat; Vazquez, Alejandro; Church, George McDonald; Shih, William (Oxford University Press, 2009)
      DNA nanotechnology exploits the programmable specificity afforded by base-pairing to produce self-assembling macromolecular objects of custom shape. For building megadalton-scale DNA nanostructures, a long ‘scaffold’ strand ...
    • Regulation at a distance of biomolecular interactions using a DNA origami nanoactuator 

      Ke, Yonggang; Meyer, Travis; Shih, William M.; Bellot, Gaetan (Nature Publishing Group, 2016)
      The creation of nanometre-sized structures that exhibit controllable motions and functions is a critical step towards building nanomachines. Recent developments in the field of DNA nanotechnology have begun to address these ...
    • Scalable amplification of strand subsets from chip-synthesized oligonucleotide libraries 

      Schmidt, Thorsten L.; Beliveau, Brian J.; Uca, Yavuz O.; Theilmann, Mark; Da Cruz, Felipe; Wu, Chao-Ting; Shih, William M. (Nature Pub. Group, 2015)
      Synthetic oligonucleotides are the main cost factor for studies in DNA nanotechnology, genetics and synthetic biology, which all require thousands of these at high quality. Inexpensive chip-synthesized oligonucleotide ...
    • Self-assembly of size-controlled liposomes on DNA nanotemplates 

      Yang, Yang; Wang, Jing; Shigematsu, Hideki; Xu, Weiming; Shih, William M; Rothman, James E; Lin, Chenxiang (2016)
      Artificial lipid-bilayer membranes are valuable tools for the study of membrane structure and dynamics. For applications such as studying vesicular transport and drug delivery, there is a pressing need for artificial ...
    • Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes 

      Beliveau, Brian J.; Boettiger, Alistair N.; Avendaño, Maier S.; Jungmann, Ralf; McCole, Ruth B.; Joyce, Eric F.; Kim-Kiselak, Caroline; Bantignies, Frédéric; Fonseka, Chamith Y.; Erceg, Jelena; Hannan, Mohammed A.; Hoang, Hien G.; Colognori, David; Lee, Jeannie T.; Shih, William M.; Yin, Peng; Zhuang, Xiaowei; Wu, Chao-ting (Nature Pub. Group, 2015)
      Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization ...
    • Synthetic organization in vitro and in vivo 

      Myhrvold, Cameron (2016-05-14)
      Organized complexity is a hallmark of biology in general, and eukaryotes in particular. This phenomenon abounds across many size scales ranging from tissues to organelles to protein complexes. Scaffold molecules, which ...