Browsing by Author "Perrimon, Norbert"

Now showing items 1-20 of 169

    • Activation of the JNK Pathway During Dorsal Closure in Drosophila Requires the Mixed Lineage Kinase, Slipper 

      Stronach, Beth E.; Perrimon, Norbert (Cold Spring Harbor Laboratory, 2002-02-01)
      The Jun kinase (JNK) pathway has been characterized for its role in stimulating AP-1 activity and for modulating the balance between cell growth and death during development, inflammation, and cancer. Six families of ...

    • Activin Signaling Mediates Muscle-to-Adipose Communication in a Mitochondria Dysfunction-Associated Obesity Model 

      Song, Wei; Owusu-Ansah, Edward; Hu, Yanhui; Cheng, Daojun; Ni, Xiaochun; Zirin, Jonathan; Perrimon, Norbert (National Academy of Sciences, 2017-08-08)
      Mitochondrial perturbation-associated dysregulation of one organ has been shown to nonautonomously affect the functions of other organs in both vertebrates and invertebrates. Using Drosophila as a genetic model organism, ...

    • All for One, and One for All: The Clonality of the Intestinal Stem Cell Niche 

      Karpowicz, Phillip Adam; Perrimon, Norbert (Faculty of 1000 Ltd, 2010)
      Intestinal epithelia are maintained by intestinal stem cells (ISCs) that divide to replace dying absorptive and secretory cells that make up this tissue. Lineage labeling studies, both in vertebrates and Drosophila, have ...

    • Altering the Insertional Specificity of a Drosophila Transposable Element 

      Kassis, Judith A.; Noll, Elizabeth; VanSickle, E. Page; Odenwald, Ward F.; Perrimon, Norbert (National Academy of Sciences, 1992-03-01)
      Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During ...

    • The Atg1-Tor Pathway Regulates Yolk Catabolism in Drosophila Embryos 

      Kuhn, Hallie; Sopko, Richelle; Coughlin, Margaret; Perrimon, Norbert; Mitchison, Timothy (The Company of Biologists, 2015-11-15)
      Yolk provides an important source of nutrients during the early development of oviparous organisms. It is composed mainly of vitellogenin proteins packed into membrane-bound compartments called yolk platelets. Catabolism ...

    • Blocking p62-dependent SMN degradation ameliorates spinal muscular atrophy disease phenotypes 

      Rodriguez-Muela, Natalia; Parkhitko, Andrey; Grass, Tobias; Gibbs, Rebecca; Norabuena, Erika M.; Perrimon, Norbert; Singh, Rajat; Rubin, Lee (American Society for Clinical Investigation, 2018-06-11)
      Spinal muscular atrophy (SMA), a degenerative motor neuron (MN) disease, caused by loss of functional survival of motor neuron (SMN) protein due to SMN1 gene mutations, is a leading cause of infant mortality. Increasing ...

    • A Case Study of the Reproducibility of Transcriptional Reporter Cell-Based RNAi Screens in Drosophila 

      DasGupta, Ramanuj; Nybakken, Kent E.; Booker, Matthew; Mathey-Prevot, Bernard; Gonsalves, Foster; Changkakoty, Binita; Perrimon, Norbert (BioMed Central, 2007)
      Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based ...

    • The Circadian Clock Gates the Intestinal Stem Cell Regenerative State 

      Karpowicz, Phillip; Zhang, Yong; Hogenesch, John B.; Emery, Patrick; Perrimon, Norbert (Elsevier BV, 2013-04-25)
      The intestine has evolved under constant environmental stresses, because an animal may ingest harmful pathogens or chemicals at any time during its lifespan. Following damage, intestinal stem cells (ISCs) regenerate the ...

    • Combining Genetic Perturbations and Proteomics to Examine Kinase-Phosphatase Networks in Drosophila Embryos 

      Sopko, Richelle; Foos, Marianna; Vinayagam, Arunachalam; Zhai, Bo; Binari, Richard; Hu, Yanhui; Randklev, Sakara; Perkins, Lizabeth A.; Gygi, Steven; Perrimon, Norbert (Elsevier BV, 2014-10-13)
      Connecting phosphorylation events to kinases and phosphatases is key to understanding the molecular organization and signaling dynamics of networks. We have generated a validated set of transgenic RNA-interference reagents ...

    • Comparative Analysis of Argonaute-Dependent Small RNA Pathways in Drosophila 

      Zhou, Rui; Hotta, Ikuko; Denli, Ahmet M.; Hong, Pengyu; Perrimon, Norbert; Hannon, Gregory J. (Elsevier BV, 2008-11-21)
      The specificity of RNAi pathways is determined by several classes of small RNAs, which include siRNAs, piRNAs, endo-siRNAs, and microRNAs (miRNAs). These small RNAs are invariably incorporated into large Argonaute ...

    • Comparative Analysis of Cas9 Activators Across Multiple Species 

      Chavez, Alejandro; Tuttle, Marcelle; Pruitt, Benjamin W; Ewen-Campen, Ben; Chari, Raj; Ter-Ovanesyan, Dmitry; Haque, Sabina J; Cecchi, Ryan J; Kowal, Emma J K; Buchthal, Joanna; Housden, Benjamin E; Perrimon, Norbert; Collins, James J; Church, George (2016)
      Several groups have generated programmable transcription factors based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare ...

    • Comparative Analysis of the Transcriptome Across Distant Species 

      Gerstein, Mark B.; Rozowsky, Joel; Yan, Koon-Kiu; Wang, Daifeng; Cheng, Chao; Brown, James B.; Davis, Carrie A.; Hillier, LaDeana; Sisu, Cristina; Li, Jingyi Jessica; Pei, Baikang; Harmanci, Arif O.; Duff, Michael O.; Djebali, Sarah; Alexander, Roger P.; Alver, Burak; Auerbach, Raymond; Bell, Kimberly; Bickel, Peter J.; Boeck, Max E.; Boley, Nathan P.; Booth, Benjamin W.; Cherbas, Lucy; Cherbas, Peter; Di, Chao; Dobin, Alex; Drenkow, Jorg; Ewing, Brent; Fang, Gang; Fastuca, Megan; Feingold, Elise A.; Frankish, Adam; Gao, Guanjun; Good, Peter J.; Guigó, Roderic; Hammonds, Ann; Harrow, Jen; Hoskins, Roger A.; Howald, Cédric; Hu, Long; Huang, Haiyan; Hubbard, Tim J. P.; Huynh, Chau; Jha, Sonali; Kasper, Dionna; Kato, Masaomi; Kaufman, Thomas C.; Kitchen, Robert R.; Ladewig, Erik; Lagarde, Julien; Lai, Eric; Leng, Jing; Lu, Zhi; MacCoss, Michael; May, Gemma; McWhirter, Rebecca; Merrihew, Gennifer; Miller, David M.; Mortazavi, Ali; Murad, Rabi; Oliver, Brian; Olson, Sara; Park, Peter; Pazin, Michael J.; Perrimon, Norbert; Pervouchine, Dmitri; Reinke, Valerie; Reymond, Alexandre; Robinson, Garrett; Samsonova, Anastasia; Saunders, Gary I.; Schlesinger, Felix; Sethi, Anurag; Slack, Frank J.; Spencer, William C.; Stoiber, Marcus H.; Strasbourger, Pnina; Tanzer, Andrea; Thompson, Owen A.; Wan, Kenneth H.; Wang, Guilin; Wang, Huaien; Watkins, Kathie L.; Wen, Jiayu; Wen, Kejia; Xue, Chenghai; Yang, Li; Yip, Kevin; Zaleski, Chris; Zhang, Yan; Zheng, Henry; Brenner, Steven E.; Graveley, Brenton R.; Celniker, Susan E.; Gingeras, Thomas R.; Waterston, Robert (Springer Science and Business Media LLC, 2014-08-27)
      The transcriptome is the readout of the genome. Identifying common features in it across distant species can reveal fundamental principles. To this end, the ENCODE and modENCODE consortia have generated large amounts of ...

    • Comparative RNAi Screening Identifies a Conserved Core Metazoan Actinome by Phenotype 

      Rohn, Jennifer L.; Sims, David; Liu, Tao; Fedorova, Marina; Schöck, Frieder; Dopie, Joseph; Vartiainen, Maria K.; Kiger, Amy A.; Perrimon, Norbert; Baum, Buzz (The Rockefeller University Press, 2011)
      Although a large number of actin-binding proteins and their regulators have been identified through classical approaches, gaps in our knowledge remain. Here, we used genome-wide RNA interference as a systematic method to ...

    • Complementary Genomic Screens Identify SERCA as a Therapeutic Target in NOTCH1 Mutated Cancer 

      Roti, Giovanni; Carlton, Anne; Ross, Kenneth; Markstein, Michele; Pajcini, Kostandin; Su, Angela H.; Perrimon, Norbert; Pear, Warren S.; Kung, Andrew L.; Blacklow, Stephen; Aster, Jon; Stegmaier, Kimberly (Elsevier BV, 2013-03-18)
      Notch1 is a rational therapeutic target in several human cancers, but as a transcriptional regulator, it poses a drug discovery challenge. To identify Notch1 modulators, we performed two cell-based, high-throughput screens ...

    • A computational framework for boosting confidence in high-throughput protein-protein interaction datasets 

      Hosur, Raghavendra; Peng, Jian; Vinayagam, Arunachalam; Stelzl, Ulrich; Xu, Jinbo; Perrimon, Norbert; Bienkowska, Jadwiga; Berger, Bonnie (BioMed Central, 2012)
      Improving the quality and coverage of the protein interactome is of tantamount importance for biomedical research, particularly given the various sources of uncertainty in high-throughput techniques. We introduce a ...

    • Conserved phosphorylation hotspots in eukaryotic protein domain families 

      Strumillo, Marta J.; Oplová, Michaela; Viéitez, Cristina; Ochoa, David; Shahraz, Mohammed; Busby, Bede P.; Sopko, Richelle; Studer, Romain A.; Perrimon, Norbert; Panse, Vikram G.; Beltrao, Pedro (Springer Science and Business Media LLC, 2019-04-29)
      Protein phosphorylation is the best characterized post-translational modification that regulates almost all cellular processes through diverse mechanisms such as changing protein conformations, interactions, and localization. ...

    • Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses 

      Neumuller, Ralph; Gross, Thomas; Samsonova, Anastasia A.; Vinayagam, Arunachalam; Buckner, Michael; Founk, Karen; Hu, Yanhui; Sharifpoor, Sara; Rosebrock, Adam P.; Andrews, Brenda; Winston, Fred; Perrimon, Norbert (American Association for the Advancement of Science (AAAS), 2013-08-20)
      Regulation of cell growth is a fundamental process in development and disease that integrates a vast array of extra- and intracellular information. A central player in this process is RNA polymerase I (Pol I), which ...

    • Control of Cell Fate Determination by p21ras/Ras1, an Essential Component of Torso Signaling in Drosophila 

      Lu, Xiangyi; Chou, Tze-Bin; Williams, Nidhi Gupta; Roberts, Thomas; Perrimon, Norbert (Cold Spring Harbor Laboratory, 1993-04-01)
      Determination of cell fate at the posterior termini of the Drosophila embryo is specified by the activation of the torso (tor) receptor tyrosine kinase. This signaling pathway is mediated by the serine/threonine kinase ...

    • Control of Lipid Metabolism by Tachykinin in Drosophila 

      Song, Wei; Veenstra, Jan A.; Perrimon, Norbert (Elsevier BV, 2014-10-09)
      The intestine is a key organ for lipid uptake and distribution, and abnormal intestinal lipid metabolism is associated with obesity and hyperlipidemia. Although multiple regulatory gut hormones secreted from enteroendocrine ...

    • Control of Proinflammatory Gene Programs by Regulated Trimethylation and Demethylation of Histone H4K20 

      Stender, Joshua D.; Pascual, Gabriel; Liu, Wen; Kaikkonen, Minna U.; Do, Kevin; Spann, Nathanael J.; Boutros, Michael; Perrimon, Norbert; Rosenfeld, Michael G.; Glass, Christopher K. (Elsevier BV, 2012-10-12)
      Regulation of genes that initiate and amplify inflammatory programs of gene expression is achieved by signal-dependent exchange of co-regulator complexes that function to read, write and erase specific histone modifications ...