Now showing items 1-20 of 33

    • Activation of Multiple Proto-oncogenic Tyrosine Kinases in Breast Cancer via Loss of the PTPN12 Phosphatase 

      Sun, Tingting; Aceto, Nicola; Meerbrey, Kristen L.; Kessler, Jessica D.; Zhou, Chunshui; Migliaccio, Ilenia; Nguyen, Don X.; Pavlova, Natalya N.; Botero, Maria; Huang, Jian; Bernardi, Ronald J.; Schmitt, Earlene; Hu, Guang; Li, Mamie Z.; Dephoure, Noah; Rao, Mitchell; Creighton, Chad J.; Hilsenbeck, Susan G.; Shaw, Chad A.; Muzny, Donna; Gibbs, Richard A.; Wheeler, David A.; Osborne, C. Kent; Schiff, Rachel; Bentires-Alj, Mohamed; Westbrook, Thomas F.; Elledge, Stephen; Gygi, Steven (Elsevier BV, 2011-03)
      Among breast cancers, triple-negative breast cancer (TNBC) is the most poorly understood and is refractory to current targeted therapies. Using a genetic screen, we identify the PTPN12 tyrosine phosphatase as a tumor ...
    • Amphotericin B Increases Influenza A Virus Infection by Preventing IFITM3-Mediated Restriction 

      Lin, Tsai-Yu; Chin, Christopher R.; Everitt, Aaron R.; Clare, Simon; Perreira, Jill M.; Savidis, George; John, Sinu P.; Sarlah, David; Carreira, Erick M.; Kellam, Paul; Brass, Abraham L.; Elledge, Stephen; Aker, Aaron; john, sinu (Elsevier BV, 2013-11)
      The IFITMs inhibit influenza A virus (IAV) replication in vitro and in vivo. Here, we establish that the antimycotic heptaen, amphotericin B (AmphoB), prevents IFITM3-mediated restriction of IAV, thereby increasing viral ...
    • Application of a synthetic human proteome to autoantigen discovery through PhIP-Seq 

      Larman, H. Benjamin; Zhao, Zhenming; Laserson, Uri; Li, Mamie Z.; Ciccia, Alberto; Gakidis, M. Angelica Martinez; Church, George M.; Kesari, Santosh; LeProust, Emily M.; Solimini, Nicole L.; Elledge, Stephen J. (2011)
      In this study, we improve on current autoantigen discovery approaches by creating a synthetic representation of the complete human proteome, the T7 “peptidome” phage display library (T7-Pep), and use it to profile the ...
    • Autoreactivity and Exceptional CDR Plasticity (but Not Unusual Polyspecificity) Hinder Elicitation of the Anti-HIV Antibody 4E10 

      Finton, Kathryn A. K.; Larimore, Kevin; Larman, H. Benjamin; Friend, Della; Correnti, Colin; Rupert, Peter B.; Elledge, Stephen J.; Greenberg, Philip D.; Strong, Roland K. (Public Library of Science, 2013)
      The broadly-neutralizing anti-HIV antibody 4E10 recognizes an epitope in the membrane-proximal external region of the HIV envelope protein gp41. Previous attempts to elicit 4E10 by vaccination with envelope-derived or ...
    • BRCA1 as tumor suppressor: lord without its RING? 

      Elia, Andrew Eugene; Elledge, Stephen J. (BioMed Central, 2012)
      BRCA1 is a tumor suppressor with critical roles in the maintenance of genomic stability. It encodes a large protein with an amino-terminal RING domain that possesses ubiquitin-ligase activity. Given the occurrence of ...
    • Brief Report: VGLL4 Is a Novel Regulator of Survival in Human Embryonic Stem Cells 

      Tajonar, Adriana; Maehr, René; Hu, Guang; Sneddon, Julie Beth; Rivera-Feliciano, Jose; Cohen, Dena Elana; Elledge, Stephen J.; Melton, Douglas A. (AlphaMed Press, 2013)
      Human embryonic stem cells (hESCs) are maintained in a self-renewing state by an interconnected network of mechanisms that sustain pluripotency, promote proliferation and survival, and prevent differentiation. We sought ...
    • Comprehensive Identification of Host Modulators of HIV-1 Replication using Multiple Orthologous RNAi Reagents 

      Zhu, Jian; Davoli, Teresa; Perriera, Jill M.; Chin, Christopher R.; Gaiha, Gaurav D.; John, Sinu P.; Sigiollot, Frederic D.; Gao, Geng; Xu, Qikai; Qu, Hongjing; Pertel, Thomas; Sims, Jennifer S.; Smith, Jennifer A.; Baker, Richard E.; Maranda, Louise; Ng, Aylwin; Elledge, Stephen J.; Brass, Abraham L. (2016)
      SUMMARY RNAi screens have implicated hundreds of host proteins as HIV-1 dependency factors (HDFs). While informative, these early studies overlap poorly due to false positives and false negatives. To ameliorate these issues, ...
    • Cyclin D1 Provides a Link Between Development and Oncogenesis in the Retina and Breast 

      Sicinski, Piotr; Donaher, Joana Liu; Parker, Susan B.; Li, Tiansen; Fazeli, Amin; Gardner, Humphrey; Haslam, Sandra Z.; Bronson, Roderick T.; Elledge, Stephen; Weinberg, Robert A. (Elsevier BV, 1995-08)
    • Cytosolic 5′-Nucleotidase 1A Autoimmunity in Sporadic Inclusion Body Myositis 

      Benjamin Larman, H.; Salajegheh, Mohammad; Nazareno, Remedios; Lam, Theresa; Sauld, John; Steen, Hanno; Won Kong, Sek; Pinkus, Jack L.; Amato, Anthony A.; Elledge, Stephen; Greenberg, Steven A. (Wiley, 2013-03)
      Objective We previously identified a circulating autoantibody against a 43 kDa muscle autoantigen in sporadic inclusion body myositis (IBM) and demonstrated the feasibility of an IBM diagnostic blood test. Here, we sought ...
    • Distinct Patterns of IFITM-Mediated Restriction of Filoviruses, SARS Coronavirus, and Influenza A Virus 

      Radoshitzky, Sheli R.; Becker, Michelle M.; Chi, Xiaoli; Dong, Lian; Longobardi, Lindsay E.; Boltz, Dutch; Kuhn, Jens H.; Bavari, Sina; Denison, Mark R.; Baric, Ralph S.; Huang, I-Chueh; Bailey, Charles; Weyer, Jessica; Chiang, Jessica; Brass, Abraham L.; Ahmed, Asim Aminsharif; Elledge, Stephen J.; Choe, Hyeryun; Farzan, Michael Reynolds (Public Library of Science, 2011)
      Interferon-inducible transmembrane proteins 1, 2, and 3 (IFITM1, 2, and 3) are recently identified viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Here ...
    • A Functional Genomics Approach to Viral-Host Interactions for HIV 

      Elledge, Stephen J. (BioMed Central, 2010)
    • Functional kinomics establishes a critical node of volume-sensitive cation-Cl− cotransporter regulation in the mammalian brain 

      Zhang, Jinwei; Gao, Geng; Begum, Gulnaz; Wang, Jinhua; Khanna, Arjun R.; Shmukler, Boris E.; Daubner, Gerrit M.; de los Heros, Paola; Davies, Paul; Varghese, Joby; Bhuiyan, Mohammad Iqbal H.; Duan, Jinjing; Zhang, Jin; Duran, Daniel; Alper, Seth L.; Sun, Dandan; Elledge, Stephen J.; Alessi, Dario R.; Kahle, Kristopher T. (Nature Publishing Group, 2016)
      Cell volume homeostasis requires the dynamically regulated transport of ions across the plasmalemma. While the ensemble of ion transport proteins involved in cell volume regulation is well established, the molecular ...
    • Functional Properties of an Ensemble of Candidate Germline-Encoded Precursors of the Anti-MPER Antibody 4E10 

      Finton, K; Larmen, B; Larimore, K; Stuart, A; Friend, D; Vanden Bos, T; Greenberg, P; Elledge, Stephen J.; Stamatatos, L; Strong, R (BioMed Central, 2012)
    • A Genetic Screen for Candidate Tumor Suppressors Identifies REST 

      Westbrook, Thomas F.; Martin, Eric S.; Schlabach, Michael R.; Leng, Yumei; Liang, Anthony C.; Feng, Bin; Zhao, Jean J.; Roberts, Thomas M.; Mandel, Gail; Hannon, Gregory J.; DePinho, Ronald A.; Chin, Lynda; Elledge, Stephen (Elsevier BV, 2005-06)
      Tumorigenesis is a multistep process characterized by a myriad of genetic and epigenetic alterations. Identifying the causal perturbations that confer malignant transformation is a central goal in cancer biology. Here we ...
    • A Genetic Screen Identifies FAN1, a Fanconi Anemia-Associated Nuclease Necessary for DNA Interstrand Crosslink Repair 

      Smogorzewska, Agata; Desetty, Rohini; Saito, Takamune; Schlabach, Michael; Lach, Francis P.; Sowa, Mathew E.; Clark, Alan B.; Kunkel, Thomas A.; Harper, Jeffrey Wade; Colaiacovo, Monica P.; Elledge, Stephen J. (Elsevier BV, 2010)
      The Fanconi anemia (FA) pathway is responsible for interstrand crosslink repair. At the heart of this pathway is the FANCI-FAND2 (ID) complex, which, upon ubiquitination by the FA core complex, travels to sites of damage ...
    • Genome-Wide Loss-of-Function Genetic Screens Identify Novel Senescence Genes and Putative Tumor Suppressors 

      Burrows, Anna (2013-02-22)
      During every cell cycle and upon exogenous stress, tumor suppression programs are engaged to ensure genomic stability. In response to replicative aging and oncogenic stimuli, the p53 and Rb pathways are activated to prevent ...
    • Genome-wide shRNA screen revealed integrated mitogenic signaling between dopamine receptor D2 (DRD2) and epidermal growth factor receptor (EGFR) in glioblastoma 

      Li, Jie; Zhu, Shan; Kozono, David; Ng, Kimberly; Futalan, Diahnn; Shen, Ying; Akers, Johnny C.; Steed, Tyler; Kushwaha, Deepa; Schlabach, Michael; Carter, Bob S.; Kwon, Chang-Hyuk; Furnari, Frank; Cavenee, Webster; Elledge, Stephen; Chen, Clark C. (Impact Journals LLC, 2014)
      Glioblastoma remains one of the deadliest of human cancers, with most patients succumbing to the disease within two years of diagnosis. The available data suggest that simultaneous inactivation of critical nodes within the ...
    • A Genome-Wide Study of Homologous Recombination in Mammalian Cells Identifies RBMX, a Novel Component of the DNA Damage Response 

      Adamson, Brittany Susan (2013-03-20)
      Repair of DNA double-strand breaks is critical to the maintenance of genomic stability, and failure to repair these DNA lesions can cause loss of chromosome telomeric regions, complex translocations, or cell death. In ...
    • Global Identification of Modular Cullin-RING Ligase Substrates 

      Emanuele, Michael J.; Xu, Qikai; Thoma, Claudio R.; Izhar, Lior; Leng, Yumei; Guo, Ailan; Chen, Yi-Ning; Rush, John; Hsu, Paul Wei-Che; Yen, Hsueh-Chi Sherry; Elledge, Stephen; Elia, Andrew (Elsevier BV, 2011-10)
      Cullin-RING ligases (CRLs) represent the largest E3 ubiquitin ligase family in eukaryotes, and the identification of their substrates is critical to understanding regulation of the proteome. Using genetic and pharmacologic ...
    • Identification of the FANCI Protein, a Monoubiquitinated FANCD2 Paralog Required for DNA Repair 

      Smogorzewska, Agata; Matsuoka, Shuhei; Vinciguerra, Patrizia; McDonald, E. Robert; Hurov, Kristen E.; Luo, Ji; Ballif, Bryan A.; Hofmann, Kay; D'Andrea, Alan D.; Elledge, Stephen; Gygi, Steven (Elsevier BV, 2007-04)
      Fanconi anemia (FA) is a developmental and cancer-predisposition syndrome caused by mutations in genes controlling DNA interstrand crosslink repair. Several FA proteins form a ubiquitin ligase that controls monoubiquitination ...