Now showing items 1-20 of 22

    • Application of a synthetic human proteome to autoantigen discovery through PhIP-Seq 

      Larman, H. Benjamin; Zhao, Zhenming; Laserson, Uri; Li, Mamie Z.; Ciccia, Alberto; Gakidis, M. Angelica Martinez; Church, George M.; Kesari, Santosh; LeProust, Emily M.; Solimini, Nicole L.; Elledge, Stephen J. (2011)
      In this study, we improve on current autoantigen discovery approaches by creating a synthetic representation of the complete human proteome, the T7 “peptidome” phage display library (T7-Pep), and use it to profile the ...
    • Autoreactivity and Exceptional CDR Plasticity (but Not Unusual Polyspecificity) Hinder Elicitation of the Anti-HIV Antibody 4E10 

      Finton, Kathryn A. K.; Larimore, Kevin; Larman, H. Benjamin; Friend, Della; Correnti, Colin; Rupert, Peter B.; Elledge, Stephen J.; Greenberg, Philip D.; Strong, Roland K. (Public Library of Science, 2013)
      The broadly-neutralizing anti-HIV antibody 4E10 recognizes an epitope in the membrane-proximal external region of the HIV envelope protein gp41. Previous attempts to elicit 4E10 by vaccination with envelope-derived or ...
    • BRCA1 as tumor suppressor: lord without its RING? 

      Elia, Andrew Eugene; Elledge, Stephen J. (BioMed Central, 2012)
      BRCA1 is a tumor suppressor with critical roles in the maintenance of genomic stability. It encodes a large protein with an amino-terminal RING domain that possesses ubiquitin-ligase activity. Given the occurrence of ...
    • Brief Report: VGLL4 Is a Novel Regulator of Survival in Human Embryonic Stem Cells 

      Tajonar, Adriana; Maehr, René; Hu, Guang; Sneddon, Julie Beth; Rivera-Feliciano, Jose; Cohen, Dena Elana; Elledge, Stephen J.; Melton, Douglas A. (AlphaMed Press, 2013)
      Human embryonic stem cells (hESCs) are maintained in a self-renewing state by an interconnected network of mechanisms that sustain pluripotency, promote proliferation and survival, and prevent differentiation. We sought ...
    • Comprehensive Identification of Host Modulators of HIV-1 Replication using Multiple Orthologous RNAi Reagents 

      Zhu, Jian; Davoli, Teresa; Perriera, Jill M.; Chin, Christopher R.; Gaiha, Gaurav D.; John, Sinu P.; Sigiollot, Frederic D.; Gao, Geng; Xu, Qikai; Qu, Hongjing; Pertel, Thomas; Sims, Jennifer S.; Smith, Jennifer A.; Baker, Richard E.; Maranda, Louise; Ng, Aylwin; Elledge, Stephen J.; Brass, Abraham L. (2016)
      SUMMARY RNAi screens have implicated hundreds of host proteins as HIV-1 dependency factors (HDFs). While informative, these early studies overlap poorly due to false positives and false negatives. To ameliorate these issues, ...
    • Distinct Patterns of IFITM-Mediated Restriction of Filoviruses, SARS Coronavirus, and Influenza A Virus 

      Radoshitzky, Sheli R.; Becker, Michelle M.; Chi, Xiaoli; Dong, Lian; Longobardi, Lindsay E.; Boltz, Dutch; Kuhn, Jens H.; Bavari, Sina; Denison, Mark R.; Baric, Ralph S.; Huang, I-Chueh; Bailey, Charles; Weyer, Jessica; Chiang, Jessica; Brass, Abraham L.; Ahmed, Asim Aminsharif; Elledge, Stephen J.; Choe, Hyeryun; Farzan, Michael Reynolds (Public Library of Science, 2011)
      Interferon-inducible transmembrane proteins 1, 2, and 3 (IFITM1, 2, and 3) are recently identified viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Here ...
    • A Functional Genomics Approach to Viral-Host Interactions for HIV 

      Elledge, Stephen J. (BioMed Central, 2010)
    • Functional kinomics establishes a critical node of volume-sensitive cation-Cl− cotransporter regulation in the mammalian brain 

      Zhang, Jinwei; Gao, Geng; Begum, Gulnaz; Wang, Jinhua; Khanna, Arjun R.; Shmukler, Boris E.; Daubner, Gerrit M.; de los Heros, Paola; Davies, Paul; Varghese, Joby; Bhuiyan, Mohammad Iqbal H.; Duan, Jinjing; Zhang, Jin; Duran, Daniel; Alper, Seth L.; Sun, Dandan; Elledge, Stephen J.; Alessi, Dario R.; Kahle, Kristopher T. (Nature Publishing Group, 2016)
      Cell volume homeostasis requires the dynamically regulated transport of ions across the plasmalemma. While the ensemble of ion transport proteins involved in cell volume regulation is well established, the molecular ...
    • Functional Properties of an Ensemble of Candidate Germline-Encoded Precursors of the Anti-MPER Antibody 4E10 

      Finton, K; Larmen, B; Larimore, K; Stuart, A; Friend, D; Vanden Bos, T; Greenberg, P; Elledge, Stephen J.; Stamatatos, L; Strong, R (BioMed Central, 2012)
    • A Genetic Screen Identifies FAN1, a Fanconi Anemia-Associated Nuclease Necessary for DNA Interstrand Crosslink Repair 

      Smogorzewska, Agata; Desetty, Rohini; Saito, Takamune; Schlabach, Michael; Lach, Francis P.; Sowa, Mathew E.; Clark, Alan B.; Kunkel, Thomas A.; Harper, Jeffrey Wade; Colaiacovo, Monica P.; Elledge, Stephen J. (Elsevier BV, 2010)
      The Fanconi anemia (FA) pathway is responsible for interstrand crosslink repair. At the heart of this pathway is the FANCI-FAND2 (ID) complex, which, upon ubiquitination by the FA core complex, travels to sites of damage ...
    • Genome-Wide Loss-of-Function Genetic Screens Identify Novel Senescence Genes and Putative Tumor Suppressors 

      Burrows, Anna (2013-02-22)
      During every cell cycle and upon exogenous stress, tumor suppression programs are engaged to ensure genomic stability. In response to replicative aging and oncogenic stimuli, the p53 and Rb pathways are activated to prevent ...
    • Genome-wide shRNA screen revealed integrated mitogenic signaling between dopamine receptor D2 (DRD2) and epidermal growth factor receptor (EGFR) in glioblastoma 

      Li, Jie; Zhu, Shan; Kozono, David; Ng, Kimberly; Futalan, Diahnn; Shen, Ying; Akers, Johnny C.; Steed, Tyler; Kushwaha, Deepa; Schlabach, Michael; Carter, Bob S.; Kwon, Chang-Hyuk; Furnari, Frank; Cavenee, Webster; Elledge, Stephen; Chen, Clark C. (Impact Journals LLC, 2014)
      Glioblastoma remains one of the deadliest of human cancers, with most patients succumbing to the disease within two years of diagnosis. The available data suggest that simultaneous inactivation of critical nodes within the ...
    • A Genome-Wide Study of Homologous Recombination in Mammalian Cells Identifies RBMX, a Novel Component of the DNA Damage Response 

      Adamson, Brittany Susan (2013-03-20)
      Repair of DNA double-strand breaks is critical to the maintenance of genomic stability, and failure to repair these DNA lesions can cause loss of chromosome telomeric regions, complex translocations, or cell death. In ...
    • NatB Domain-Containing CRA-1 Antagonizes Hydrolase ACER-1 Linking Acetyl-CoA Metabolism to the Initiation of Recombination during C. elegans Meiosis 

      Gao, Jinmin; Kim, Hyun-Min; Elia, Andrew E.; Elledge, Stephen J.; Colaiácovo, Monica P. (Public Library of Science, 2015)
      The formation of DNA double-strand breaks (DSBs) must take place during meiosis to ensure the formation of crossovers, which are required for accurate chromosome segregation, therefore avoiding aneuploidy. However, DSB ...
    • Ontogeny of Recognition Specificity and Functionality for the Broadly Neutralizing Anti-HIV Antibody 4E10 

      Finton, Kathryn A. K.; Friend, Della; Jaffe, James; Gewe, Mesfin; Holmes, Margaret A.; Larman, H. Benjamin; Stuart, Andrew; Larimore, Kevin; Greenberg, Philip D.; Elledge, Stephen J.; Stamatatos, Leonidas; Strong, Roland K. (Public Library of Science, 2014)
      The process of antibody ontogeny typically improves affinity, on-rate, and thermostability, narrows polyspecificity, and rigidifies the combining site to the conformer optimal for binding from the broader ensemble accessible ...
    • Origins of lymphatic and distant metastases in human colorectal cancer 

      Naxerova, Kamila; Reiter, Johannes; Brachtel, Elena F.; Lennerz, Jochen Klaus Maria; van de Wetering, Marc; Rowan, Andrew; Cai, Tianxi; Clevers, Hans; Swanton, Charles; Nowak, Martin A.; Elledge, Stephen J.; Jain, Rakesh K. (American Association for the Advancement of Science (AAAS), 2017)
      The spread of cancer cells from primary tumors to regional lymph nodes is often associated with reduced survival. One prevailing model to explain this association posits that fatal, distant metastases are seeded by lymph ...
    • Protein interaction mapping with ribosome-displayed using PLATO ORF libraries 

      Zhu, Jian; Larman, H. Benjamin; Gao, Geng; Somwar, Romel; Zhang, Zijuan; Laserson, Uri; Ciccia, Alberto; Pavlova, Natalya; Church, George; Zhang, Wei; Kesari, Santosh; Elledge, Stephen J. (2013)
      Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library ...
    • A Role for PVRL4-Driven Cell-Cell Interactions in Tumorigenesis 

      Pavlova, Natalya Nickolayevna (2013-03-18)
      Deciphering genetic determinants of tumorigenesis is the greatest challenge and promise of the present-day era of biomedical research. As extensive tumor genome characterization efforts of the past decade had revealed, ...
    • Sources of Error in Mammalian Genetic Screens 

      Sack, Laura Magill; Davoli, Teresa; Xu, Qikai; Li, Mamie Z.; Elledge, Stephen J. (Genetics Society of America, 2016)
      Genetic screens are invaluable tools for dissection of biological phenomena. Optimization of such screens to enhance discovery of candidate genes and minimize false positives is thus a critical aim. Here, we report several ...
    • Systematic Identification of Synergistic Drug Pairs Targeting HIV 

      Tan, Xu; Hu, Long; Luquette, Lovelace J.; Gao, Geng; Liu, Yifang; Qu, Hongjing; Xi, Ruibin; Lu, Zhi John; Park, Peter J.; Elledge, Stephen J. (2012)
      The systematic identification of effective drug combinations has been hindered by the unavailability of methods that can explore the large combinatorial search space of drug interactions. Here we present a multiplex screening ...