Browsing by Author "Asara, John"

Now showing items 1-20 of 28

    • Amplification of phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis 

      Locasale, J.; Grassian, Alexandra; Beroukhim, Rameen; Meyerson, Matthew Langer; Wagner, Gerhard; Asara, John M.; Brugge, Joan S.; Vander Heiden, Matthew George; Cantley, Lewis C. (BioMed Central, 2012)

    • Casein Kinase 1 Proteomics Reveal Prohibitin 2 Function in Molecular Clock 

      Kategaya, Lorna S.; Hilliard, Aisha; Zhang, Louying; Asara, John M; Ptáček, Louis J.; Fu, Ying-Hui (Public Library of Science, 2012)
      Throughout the day, clock proteins synchronize changes in animal physiology (e.g., wakefulness and appetite) with external cues (e.g., daylight and food). In vertebrates, both casein kinase 1 delta and epsilon (CK1\(\delta\) ...

    • CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base 

      Wang, Won-Jing; Tay, Hwee Goon; Soni, Rajesh; Perumal, Geoffrey S.; Goll, Mary G.; Macaluso, Frank P.; Asara, John M.; Amack, Jeffrey D.; Tsou, Meng-Fu Bryan (2013)
      The transition zone (TZ) is a specialized compartment found at the base of cilia, adjacent to the centriole distal end, where axonemal microtubules (MTs) are heavily cross-linked to the surrounding membrane to form a barrier ...

    • Comprehensive metabolome analyses reveal N-acetylcysteine-responsive accumulation of kynurenine in systemic lupus erythematosus: implications for activation of the mechanistic target of rapamycin 

      Perl, Andras; Hanczko, Robert; Lai, Zhi-Wei; Oaks, Zachary; Kelly, Ryan; Borsuk, Rebecca; Asara, John M.; Phillips, Paul E. (Springer US, 2015)
      Systemic lupus erythematosus (SLE) patients exhibit depletion of the intracellular antioxidant glutathione and downstream activation of the metabolic sensor, mechanistic target of rapamycin (mTOR). Since reversal of ...

    • A Cross-Species Study of PI3K Protein-Protein Interactions Reveals the Direct Interaction of P85 and SHP2 

      Breitkopf, Susanne B.; Yang, Xuemei; Begley, Michael J.; Kulkarni, Meghana; Chiu, Yu-Hsin; Turke, Alexa B.; Lauriol, Jessica; Yuan, Min; Qi, Jie; Engelman, Jeffrey A.; Hong, Pengyu; Kontaridis, Maria I.; Cantley, Lewis C.; Perrimon, Norbert; Asara, John M. (Nature Publishing Group, 2016)
      Using a series of immunoprecipitation (IP) – tandem mass spectrometry (LC-MS/MS) experiments and reciprocal BLAST, we conducted a fly-human cross-species comparison of the phosphoinositide-3-kinase (PI3K) interactome in a ...

    • Endocrine and Metabolic Pathways Linked to Keratoconus: Implications for the Role of Hormones in the Stromal Microenvironment 

      McKay, Tina B; Hjortdal, Jesper; Sejersen, Henrik; Asara, John M; Wu, Jennifer; Karamichos, Dimitrios (Nature Publishing Group, 2016)
      Hormones play a critical role in regulating tissue function by promoting cell survival, proliferation, and differentiation. Our study explores the influence of endocrine function in regulating metabolism and inflammatory ...

    • An enolase inhibitor for the targeted treatment of ENO1-deleted cancers 

      Lin, Yu-Hsi; Satani, Nikunj; Hammoudi, Naima; Yan, Victoria C.; Barekatain, Yasaman; Khadka, Sunada; Ackroyd, Jeffrey J.; Georgiou, Dimitra K.; Pham, Cong-Dat; Arthur, Kenisha; Maxwell, David; Peng, Zhenghong; Leonard, Paul G.; Czako, Barbara; Pisaneschi, Federica; Mandal, Pijus; Sun, Yuting; Zielinski, Rafal; Pando, Susana Castro; Wang, Xiaobo; Tran, Theresa; Xu, Quanyu; Wu, Qi; Jiang, Yongying; Kang, Zhijun; Asara, John; Priebe, Waldemar; Bornmann, William; Marszalek, Joseph R.; DePinho, Ronald A.; Muller, Florian L. (Springer Science and Business Media LLC, 2020-11-23)
      Inhibiting glycolysis remains an aspirational approach for the treatment of cancer. We previously identified a subset of cancers harboring homozygous deletion of the glycolytic enzyme Enolase (ENO1) with exceptional ...

    • Ex Vivo and in Vivo Stable Isotope Labelling of Central Carbon Metabolism and Related Pathways With Analysis by LC–MS/MS 

      Yuan, Min; Kremer, Daniel M.; Huang, He; Breitkopf, Susanne B.; Ben-Sahra, Issam; Manning, Brendan; Lyssiotis, Costas A.; Asara, John (Springer Science and Business Media LLC, 2019-02)
      Targeted tandem mass spectrometry (LC–MS/MS) has been extremely useful for profiling small molecules extracted from biological sources, such as cells, bodily fluids and tissues. Here, we present a protocol for analysing ...

    • Glutamine supports pancreatic cancer growth through a Kras-regulated metabolic pathway 

      Son, Jaekyoung; Lyssiotis, Costas A.; Ying, Haoqiang; Wang, Xiaoxu; Hua, Sujun; Ligorio, Matteo; Perera, Rushika M.; Ferrone, Cristina R.; Mullarky, Edouard; Shyh-Chang, Ng; Kang, Ya’an; Fleming, Jason B.; Bardeesy, Nabeel; Asara, John M.; Haigis, Marcia C.; DePinho, Ronald A.; Cantley, Lewis C.; Kimmelman, Alec C. (2013)
      Cancer cells exhibit metabolic dependencies that distinguish them from their normal counterparts1. Among these addictions is an increased utilization of the amino acid glutamine (Gln) to fuel anabolic processes2. Indeed, ...

    • Glutathione biosynthesis is a metabolic vulnerability in PI3K/Akt-driven breast cancer 

      Lien, Evan C.; Lyssiotis, Costas A.; Juvekar, Ashish; Hu, Hai; Asara, John M.; Cantley, Lewis C.; Toker, Alex (2016)
      Cancer cells often select for mutations that enhance signaling through pathways that promote anabolic metabolism1. Although the PI3K/Akt signaling pathway, which is frequently dysregulated in breast cancer2, is a ...

    • Gross Cystic Disease Fluid Protein-15/Prolactin-Inducible Protein as a Biomarker for Keratoconus Disease 

      Priyadarsini, Shrestha; Hjortdal, Jesper; Sarker-Nag, Akhee; Sejersen, Henrik; Asara, John M.; Karamichos, Dimitrios (Public Library of Science, 2014)
      Keratoconus (KC) is a bilateral degenerative disease of the cornea characterized by corneal bulging, stromal thinning, and scarring. The etiology of the disease is unknown. In this study, we identified a new biomarker for ...

    • An Integrative Analysis of the InR/PI3K/Akt Network Identifies the Dynamic Response to Insulin Signaling 

      Vinayagam, Arunachalam; Kulkarni, Meghana; Sopko, Richelle; Sun, Xiaoyun; Hu, Yanhui; Nand, Ankita; Villalta, Christians; Moghimi, Ahmadali; Yang, Xuemei; Mohr, Stephanie; Hong, Pengyu; Asara, John; Perrimon, Norbert (Elsevier BV, 2016-09-13)
      Insulin regulates an essential conserved signaling pathway affecting growth, proliferation and metabolism. To expand our understanding of the insulin pathway, we combine biochemical, genetic and computational approaches ...

    • mTORC1 Couples Nucleotide Synthesis to Nucleotide Demand Resulting in a Targetable Metabolic Vulnerability 

      Valvezan, Alexander J.; Turner, Marc; Belaid, Amine; Lam, Hilaire; Miller, Spencer K.; Mcnamara, Molly; Baglini, Christian; Housden, Benjamin; Perrimon, Norbert; Kwiatkowski, David; Asara, John; Henske, Elizabeth; Manning, Brendan (Elsevier BV, 2017-11-13)
      The mechanistic target of rapamycin complex 1 (mTORC1) supports proliferation through parallel induction of key anabolic processes, including protein, lipid, and nucleotide synthesis. We hypothesized that these processes ...

    • Pathological glycogenesis through glycogen synthase 1 and suppression of excessive AMP kinase activity in myeloid leukemia cells 

      Bhanot, Haymanti; Reddy, Mamatha M.; Nonami, Atsushi; Weisberg, Ellen L.; Bonal, Dennis; Kirschmeier, Paul T.; Salgia, Sabrina; Podar, Klaus; Galinsky, Ilene; Chowdary, Tirumala K.; Neuberg, Donna; Tonon, Giovanni; Stone, Richard M.; Asara, John; Griffin, James D.; Sattler, Martin (2015)
      The rapid proliferation of myeloid leukemia cells is highly dependent on increased glucose metabolism. Through an unbiased metabolomics analysis of leukemia cells, we found that the glycogenic precursor UDP-D-glucose is ...

    • PGC-1α mediates mitochondrial biogenesis and oxidative phosphorylation to promote metastasis 

      LeBleu, Valerie S.; O'Connell, Joyce T.; Herrera, Karina N. Gonzalez; Wikman-Kocher, Harriet; Pantel, Klaus; Haigis, Marcia C.; de Carvalho, Fernanda Machado; Damascena, Aline; Chinen, Ludmilla Thome Domingos; Rocha, Rafael M.; Asara, John M.; Kalluri, Raghu (2014)
      Cancer cells can divert metabolites into anabolic pathways to support their rapid proliferation and to accumulate the cellular building blocks required for tumor growth. However, the specific bioenergetic profile of invasive ...

    • Polymerase I and Transcript Release Factor Regulates Lipolysis via a Phosphorylation-Dependent Mechanism 

      Aboulaich, Nabila; Chui, Patricia C.; Asara, John M; Flier, Jeffrey S.; Maratos-Flier, Eleftheria (American Diabetes Association, 2011)
      OBJECTIVE: Polymerase I and transcript release factor (PTRF) is a protein highly expressed in adipose tissue and is an integral structural component of caveolae. Here, we report on a novel role of PTRF in lipid mobilization. ...

    • Protein Tyrosine Kinase 6 Protects Cells from Anoikis by Directly Phosphorylating Focal Adhesion Kinase and Activating AKT 

      Zheng, Yu; Gierut, Jessica; Wang, Zebin; Miao, Jianjun; Asara, John M.; Tyner, Angela L. (2014)
      Protein tyrosine kinase 6 (PTK6) is a non-receptor tyrosine kinase expressed in epithelial cancers. Disruption of Ptk6 decreases AOM-induced colon tumorigenesis in mice by preventing STAT3 activation. Relocalization of ...

    • Proteomic and Functional Genomic Landscape of Receptor Tyrosine Kinase and Ras to Extracellular Signal-Regulated Kinase Signaling 

      Friedman, Adam A.; Tucker, George; Singh, Rohit; Yan, Dong; Vinayagam, Arunachalam; Hu, Yanhui; Binari, Richard; Hong, Pengyu; Sun, Xiaoyun; Porto, Maura; Pacifico, Svetlana; Murali, Thilakam; Finley, Russell L.; Asara, John; Berger, Bonnie; Perrimon, Norbert (American Association for the Advancement of Science (AAAS), 2011-10-25)
      Characterizing the extent and logic of signaling networks is essential to understanding specificity in such physiological and pathophysiological contexts as cell fate decisions and mechanisms of oncogenesis and resistance ...

    • Proteomic and Metabolomic Characterization of a Mammalian Cellular Transition from Quiescence to Proliferation 

      Lee, Ho-Joon; Jedrychowski, Mark; Vinayagam, Arunachalam; Wu, Ning; Shyh-Chang, Ng; Hu, Yanhui; Min-Wen, Chua; Moore, Jodene; Asara, John; Lyssiotis, Costas A.; Perrimon, Norbert; Gygi, Steven; Cantley, Lewis C.; Kirschner, Marc (Elsevier BV, 2017-07-18)
      There exist similarities and differences in metabolism and physiology between normal proliferative cells and tumor cells. Once a cell enters the cell cycle, metabolic machinery is engaged to facilitate various processes. ...

    • Purine and pyrimidine metabolism: Convergent evidence on chronic antidepressant treatment response in mice and humans 

      Park, Dong Ik; Dournes, Carine; Sillaber, Inge; Uhr, Manfred; Asara, John M.; Gassen, Nils C.; Rein, Theo; Ising, Marcus; Webhofer, Christian; Filiou, Michaela D.; Müller, Marianne B.; Turck, Christoph W. (Nature Publishing Group, 2016)
      Selective Serotonin Reuptake Inhibitors (SSRIs) are commonly used drugs for the treatment of psychiatric diseases including major depressive disorder (MDD). For unknown reasons a substantial number of patients do not show ...