Now showing items 1-20 of 31

    • Activation of mechanosensitive ion channel TRPV4 normalizes tumor vasculature and improves cancer therapy 

      Adapala, Ravi K.; Thoppil, Roslin J.; Ghosh, Kaustabh; Cappelli, Holly; Dudley, Andrew C.; Paruchuri, Sailaja; Keshamouni, Venkateshwar; Klagsbrun, Michael; Meszaros, J. Gary; Chilian, William M.; Ingber, Donald E.; Thodeti, Charles K. (2016)
      Tumor vessels are characterized by abnormal morphology and hyper-permeability that together cause inefficient delivery of chemotherapeutic agents. Although VEGF has been established as a critical regulator of tumor ...
    • Assessment of whole blood thrombosis in a microfluidic device lined by fixed human endothelium 

      Jain, Abhishek; van der Meer, Andries D.; Papa, Anne-Laure; Barrile, Riccardo; Lai, Angela; Schlechter, Benjamin L.; Otieno, Monicah A.; Louden, Calvert S.; Hamilton, Geraldine A.; Michelson, Alan D.; Frelinger, Andrew L.; Ingber, Donald E. (Springer US, 2016)
      The vascular endothelium and shear stress are critical determinants of physiological hemostasis and platelet function in vivo, yet current diagnostic and monitoring devices do not fully incorporate endothelial function ...
    • A bioinspired omniphobic surface coating on medical devices prevents thrombosis and biofouling 

      Leslie, Daniel; Waterhouse, Anna; Berthet, Julia B; Valentin, Thomas M; Watters, Alexander; Jain, Abhishek; Kim, Philseok; Hatton, Benjamin D; Nedder, Arthur; Donovan, Kathryn; Super, Elana H; Howell, Caitlin; Johnson, Christopher P; Vu, Thy L; Bolgen, Dana; Rifai, Sami; Hansen, Anne Rosenfeld; Aizenberg, Michael; Super, Michael; Aizenberg, Joanna; Ingber, Donald Elliot (Nature Publishing Group, 2014)
      Thrombosis and biofouling of extracorporeal circuits and indwelling medical devices cause significant morbidity and mortality worldwide. We describe a bioinspired coating that repels blood from virtually any material by ...
    • Biomechanical forces promote blood development through prostaglandin E2 and the cAMP–PKA signaling axis 

      Diaz, Miguel F.; Li, Nan; Lee, Hyun Jung; Adamo, Luigi; Evans, Siobahn M.; Willey, Hannah E.; Arora, Natasha; Torisawa, Yu-suke; Vickers, Dwayne A.; Morris, Samantha A.; Naveiras, Olaia; Murthy, Shashi K.; Ingber, Donald E.; Daley, George Q.; García-Cardeña, Guillermo; Wenzel, Pamela L. (The Rockefeller University Press, 2015)
      Blood flow promotes emergence of definitive hematopoietic stem cells (HSCs) in the developing embryo, yet the signals generated by hemodynamic forces that influence hematopoietic potential remain poorly defined. Here we ...
    • Biophysical and Molecular Determinants in Cell Tension-Mediated Fibronectin Unfolding that Drive Fibrillogenesis 

      Gee, Elaine Pei-San (2013-02-20)
      Assembly of the extracellular matrix (ECM) protein fibronectin (FN) is a mechanical process that involves cell binding to FN through cell surface integrin receptors and application of tensional forces generated in the ...
    • Bone Marrow–on–a–Chip Replicates Hematopoietic Niche Physiology in Vitro 

      Torisawa, Yu-suke; Spina, Catherine; Mammoto, Tadanori; Mammoto, Akiko; Weaver, James C.; Tat, Tracy; Collins, James J.; Ingber, Donald Elliot (Nature Publishing Group, 2014)
      Current in vitro hematopoiesis models fail to demonstrate the cellular diversity and complex functions of living bone marrow; hence, most translational studies relevant to the hematologic system are conducted in live ...
    • A Broad-Spectrum Infection Diagnostic that Detects Pathogen-Associated Molecular Patterns (PAMPs) in Whole Blood 

      Cartwright, Mark; Rottman, Martin; Shapiro, Nathan I.; Seiler, Benjamin; Lombardo, Patrick; Gamini, Nazita; Tomolonis, Julie; Watters, Alexander L.; Waterhouse, Anna; Leslie, Dan; Bolgen, Dana; Graveline, Amanda; Kang, Joo H.; Didar, Tohid; Dimitrakakis, Nikolaos; Cartwright, David; Super, Michael; Ingber, Donald E. (Elsevier, 2016)
      Background: Blood cultures, and molecular diagnostic tests that directly detect pathogen DNA in blood, fail to detect bloodstream infections in most infected patients. Thus, there is a need for a rapid test that can diagnose ...
    • A combinatorial cell-laden gel microarray for inducing osteogenic differentiation of human mesenchymal stem cells 

      Dolatshahi-Pirouz, Alireza; Nikkhah, Mehdi; Gaharwar, Akhilesh K.; Hashmi, Basma; Guermani, Enrico; Aliabadi, Hamed; Camci-Unal, Gulden; Ferrante, Thomas; Foss, Morten; Ingber, Donald E.; Khademhosseini, Ali (Nature Publishing Group, 2014)
      Development of three dimensional (3D) microenvironments that direct stem cell differentiation into functional cell types remains a major challenge in the field of regenerative medicine. Here, we describe a new platform to ...
    • Developmentally-Inspired Engineering Of An Inductive Biomaterial for Odontogenesis 

      Hashmi, Basma (2014-06-06)
      Increasing demands for organ transplants and the depleting supply of available organs has heightened the need for alternatives to this growing problem. Tissue engineers strive to regenerate organs in the future; however ...
    • Developmentally-Inspired Shrink-Wrap Polymers for Mechanical Induction of Tissue Differentiation 

      Hashmi, Basma; Zarzar, Lauren D.; Mammoto, Tadanori; Mammoto, Akiko; Jiang, Amanda; Aizenberg, Joanna; Ingber, Donald Elliot (Wiley, 2014)
      A biologically inspired thermoresponsive polymer has been developed that mechanically induces tooth differentiation in vitro and in vivo by promoting mesenchymal cell compaction as seen in each pore of the scaffold. This ...
    • Distinct Contributions of Astrocytes and Pericytes to Neuroinflammation Identified in a 3D Human Blood-Brain Barrier on a Chip 

      Herland, Anna; van der Meer, Andries D.; FitzGerald, Edward A.; Park, Tae-Eun; Sleeboom, Jelle J. F.; Ingber, Donald E. (Public Library of Science, 2016)
      Neurovascular inflammation is a major contributor to many neurological disorders, but modeling these processes in vitro has proven to be difficult. Here, we microengineered a three-dimensional (3D) model of the human ...
    • Epoxyeicosanoids stimulate multiorgan metastasis and tumor dormancy escape in mice 

      Panigrahy, Dipak; Edin, Matthew L.; Lee, Craig R.; Huang, Sui; Bielenberg, Diane; Butterfield, Catherine E.; Barnés, Carmen M.; Mammoto, Akiko; Mammoto, Tadanori; Luria, Ayala; Benny, Ofra; Chaponis, Deviney M.; Dudley, Andrew C.; Greene, Emily R.; Vergilio, Jo-Anne; Pietramaggiori, Giorgio; Scherer-Pietramaggiori, Sandra S.; Short, Sarah M.; Seth, Meetu; Lih, Fred B.; Tomer, Kenneth B.; Yang, Jun; Schwendener, Reto A.; Hammock, Bruce D.; Falck, John R.; Manthati, Vijaya L.; Ingber, Donald Elliot; Kaipainen, Arja; D'Amore, Patricia Ann; Kieran, Mark W.; Zeldin, Darryl C. (American Society for Clinical Investigation, 2012)
      Epoxyeicosatrienoic acids (EETs) are small molecules produced by cytochrome P450 epoxygenases. They are lipid mediators that act as autocrine or paracrine factors to regulate inflammation and vascular tone. As a result, ...
    • Extracellular Matrix as a Key Mediator of Mammary Tumor Cell Normalization 

      Bischof, Ashley Gibbs (2013-10-08)
      Some epithelial cancers can be induced to revert to quiescent differentiated tissues when combined with embryonic mesenchyme; however, the mechanism of this induction is unknown. This dissertation is based on the hypothesis ...
    • Fibronectin Unfolding Revisited: Modeling Cell Traction-Mediated Unfolding of the Tenth Type-III Repeat 

      Gee, Elaine Pei-San; Ingber, Donald Elliott; Stultz, Collin Melveton (Public Library of Science, 2008)
      Fibronectin polymerization is essential for the development and repair of the extracellular matrix. Consequently, deciphering the mechanism of fibronectin fibril formation is of immense interest. Fibronectin fibrillogenesis ...
    • High-Betweenness Proteins in the Yeast Protein Interaction Network 

      Joy, Maliackal Poulo; Brock, Amy Monique Lepre; Ingber, Donald Elliott; Huang, Sui (Hindawi Publishing Corporation, 2005)
      Structural features found in biomolecular networks that are absent in random networks produced by simple algorithms can provide insight into the function and evolution of cell regulatory networks. Here we analyze “betweenness” ...
    • How Changes in Extracellular Matrix Mechanics and Gene Expression Variability Might Combine to Drive Cancer Progression 

      Werfel, Justin; Krause, Silva; Bischof, Ashley G.; Mannix, Robert J.; Tobin, Heather; Bar-Yam, Yaneer; Bellin, Robert M.; Ingber, Donald E. (Public Library of Science, 2013)
      Changes in extracellular matrix (ECM) structure or mechanics can actively drive cancer progression; however, the underlying mechanism remains unknown. Here we explore whether this process could be mediated by changes in ...
    • Identification of a Distinct Class of Cytoskeleton-Associated mRNAs Using Microarray Technology 

      Brock, Amy Monique Lepre; Huang, Sui; Ingber, Donald Elliott (BioMed Central, 2003)
      Background: Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form ...
    • Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland 

      Krause, Silva; Brock, Amy; Ingber, Donald E. (MyJove Corporation, 2013)
      Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of genes within the mammary epithelium has been difficult to achieve ...
    • LRP5 Regulates Development of Lung Microvessels and Alveoli through the Angiopoietin-Tie2 Pathway 

      Mammoto, Tadanori; Jiang, Amanda Rose; Jiang, Elizabeth Qingyu; Mammoto, Akiko; Chen, Jing; Smith, Lois Elaine Hodgson; Ingber, Donald Elliott (Public Library of Science, 2012)
      Angiogenesis is crucial for lung development. Although there has been considerable exploration, the mechanism by which lung vascular and alveolar formation is controlled is still not completely understood. Here we show ...
    • Matrix Elasticity of Void-Forming Hydrogels Controls Transplanted Stem Cell-Mediated Bone Formation 

      Huebsch, Nathaniel; Lippens, Evi; Lee, Kangwon; Mehta, Manav; Koshy, Sandeep T; Darnell, Max C; Desai, Rajiv; Madl, Christopher M.; Xu, Maria; Zhao, Xuanhe; Chaudhuri, Ovijit; Verbeke, Catia; Kim, Woo Seob; Alim, Karen; Mammoto, Akiko; Ingber, Donald E.; Duda, Georg N; Mooney, David J. (2015)
      The effectiveness of stem-cell therapies has been hampered by cell death and limited control over fate1. These problems can be partially circumvented by using macroporous biomaterials that improve the survival of transplanted ...