Browsing by Author "Fu, Yanfang"
Now showing items 1-5 of 5
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CRISPR RNA-guided activation of endogenous human genes
Maeder, Morgan L; Linder, Samantha J; Cascio, Vincent M; Fu, Yanfang; Ho, Quan H; Joung, J Keith (2013)Catalytically inactive CRISPR-associated 9 nuclease (dCas9) can be directed by short guide RNAs (gRNAs) to repress endogenous genes in bacteria and human cells. Here we show that a dCas9-VP64 transcriptional activation ... -
Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
Hwang, Woong Y.; Fu, Yanfang; Reyon, Deepak; Maeder, Morgan L.; Tsai, Shengdar Q.; Sander, Jeffry D.; Peterson, Randall T.; Yeh, J.-R. Joanna; Joung, J. Keith (2013)Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have evolved in bacteria and archaea as a defense mechanism to silence foreign nucleic acids of viruses and plasmids. Recent ... -
Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
Hwang, Woong Y.; Fu, Yanfang; Reyon, Deepak; Maeder, Morgan L.; Kaini, Prakriti; Sander, Jeffry D.; Joung, J. Keith; Peterson, Randall T.; Yeh, Jing-Ruey Joanna (Public Library of Science, 2013)We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic ... -
High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
Fu, Yanfang; Foden, Jennifer A.; Khayter, Cyd; Maeder, Morgan L.; Reyon, Deepak; Joung, J. Keith; Sander, Jeffry D. (2013)CRISPR RNA-guided endonucleases (RGENs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell-based reporter assay to characterize off-target cleavage of Cas9-based RGENs. ... -
Robust, synergistic regulation of human gene expression using TALE activators
Maeder, Morgan L.; Linder, Samantha J.; Reyon, Deepak; Angstman, James F.; Fu, Yanfang; Sander, Jeffry D.; Joung, J. Keith (2013)Artificial transcription activator-like (TAL) effector-based activators (TALE activators) have broad utility but previous studies suggest that these monomeric proteins often possess low activities. Here we demonstrate that ...