Now showing items 1-7 of 7

    • Bypass of a protein roadblock by a replicative DNA helicase 

      Yardimci, Hasan; Wang, Xindan; Loveland, Anna B.; Tappin, Inger; Rudner, David Z.; Hurwitz, Jerard; van Oijen, Antoine M.; Walter, Johannes C. (2012)
      Replicative DNA helicases generally unwind DNA as a single hexamer that encircles and translocates along one strand of the duplex while excluding the complementary strand (“steric exclusion”). In contrast, large T antigen ...
    • Drugs That Thwart Antibiotic Resistance 

      Stone, Laura (2015-05-20)
      Antibiotics are often credited with being one of the major forces behind the expansion of human life expectancy in the past 60 years. Yet at the root of this advancement lies its potential undoing: using antibiotics promotes ...
    • High-Throughput Genetic Screens Identify a Large and Diverse Collection of New Sporulation Genes in Bacillus subtilis 

      Meeske, Alexander J.; Rodrigues, Christopher D. A.; Brady, Jacqueline; Lim, Hoong Chuin; Bernhardt, Thomas G.; Rudner, David Z. (Public Library of Science, 2016)
      The differentiation of the bacterium Bacillus subtilis into a dormant spore is among the most well-characterized developmental pathways in biology. Classical genetic screens performed over the past half century identified ...
    • Novel Secretion Apparatus Maintains Spore Integrity and Developmental Gene Expression in Bacillus subtilis 

      Doan, Thierry; Morlot, Cecile; Meisner, Jeffrey; Serrano, Monica; Henriques, Adriano O.; Moran, Charles P. Jr.; Rudner, David Z. (Public Library of Science, 2009)
      Sporulation in Bacillus subtilis involves two cells that follow separate but coordinately regulated developmental programs. Late in sporulation, the developing spore (the forespore) resides within a mother cell. The ...
    • Reconstitution of Peptidoglycan Cross-Linking Leads to Improved Fluorescent Probes of Cell Wall Synthesis 

      Lebar, Matthew D.; May, Janine M.; Meeske, Alexander J.; Leiman, Sara A.; Lupoli, Tania J.; Tsukamoto, Hirokazu; Losick, Richard; Rudner, David Z.; Walker, Suzanne; Kahne, Daniel (American Chemical Society, 2014)
      The peptidoglycan precursor, Lipid II, produced in the model Gram-positive bacterium Bacillus subtilis differs from Lipid II found in Gram-negative bacteria such as Escherichia coli by a single amidation on the peptide ...
    • A sigma factor and anti-sigma factor that control swarming motility and biofilm formation in Pseudomonas aeruginosa 

      McGuffie, Bryan A. (2015-05-20)
      Pseudomonas aeruginosa is an environmental bacterium and opportunistic human pathogen of major clinical significance. It is the principal cause of morbidity and mortality in patients with cystic fibrosis (CF) and a leading ...
    • Systematic approaches to deciphering genes and ecosystems 

      Kelsic, Eric David (2015-04-03)
      In this thesis I investigate how the individual components of biological systems interact, and how the form of these interactions determines overall system behavior. The interactions I study occur at widely different scales: ...