Browsing by Author "Briggs, Adrian"

Now showing items 1-5 of 5

    • Genetic Evidence of Human Adaptation to a Cooked Diet 

      Carmody, Rachel N.; Dannemann, Michael; Briggs, Adrian W.; Nickel, Birgit; Groopman, Emily E.; Wrangham, Richard W.; Kelso, Janet (Oxford University Press, 2016)
      Humans have been argued to be biologically adapted to a cooked diet, but this hypothesis has not been tested at the molecular level. Here, we combine controlled feeding experiments in mice with comparative primate genomics ...

    • Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers 

      Briggs, Adrian; Rios, Xavier; Chari, Rajagopal; Yang, Luhan; Zhang, Feng; Mali, Prashant; Church, George McDonald (Oxford University Press, 2012)
      DNA built from modular repeats presents a challenge for gene synthesis. We present a solid surface-based sequential ligation approach, which we refer to as iterative capped assembly (ICA), that adds DNA repeat monomers ...

    • Massively parallel sequencing of single cells by epicPCR links functional genes with phylogenetic markers 

      Spencer, Sarah J; Tamminen, Manu V; Preheim, Sarah P; Guo, Mira T; Briggs, Adrian W; Brito, Ilana L; A Weitz, David; Pitkänen, Leena K; Vigneault, Francois; Virta, Marko PJuhani; Alm, Eric J (Nature Publishing Group, 2016)
      Many microbial communities are characterized by high genetic diversity. 16S ribosomal RNA sequencing can determine community members, and metagenomics can determine the functional diversity, but resolving the functional ...

    • Optimization of scarless human stem cell genome editing 

      Yang, Luhan; Guell, Marc; Byrne, Susan; Yang, Joyce L.; De Los Angeles, Alejandro; Mali, Prashant; Aach, John; Kim-Kiselak, Caroline; Briggs, Adrian W; Rios, Xavier; Huang, Po-Yi; Daley, George; Church, George (Oxford University Press, 2013)
      Efficient strategies for precise genome editing in human-induced pluripotent cells (hiPSCs) will enable sophisticated genome engineering for research and clinical purposes. The development of programmable sequence-specific ...

    • Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases 

      Rios, Xavier; Briggs, Adrian; Christodoulou, Danos C.; Gorham, Josh McClean; Seidman, Jonathan G.; Church, George McDonald (Public Library of Science, 2012)
      Recent advances allow multiplexed genome engineering in E. coli, employing easily designed oligonucleotides to edit multiple loci simultaneously. A similar technology in human cells would greatly expedite functional genomics, ...