Now showing items 1-17 of 17

    • Dynorphin Activates Quorum Sensing Quinolone Signaling in Pseudomonas aeruginosa 

      Zaborina, Olga; Lepine, Francois; Xiao, Gaoping; Valuckaite, Vesta; Chen, Yimei; Li, Terry; Ciancio, Mae; Zaborin, Alex; Petroff, Elaine; Turner, Jerrold R; Chang, Eugene; Alverdy, John C; Rahme, Laurence G. (Public Library of Science, 2007)
      There is now substantial evidence that compounds released during host stress directly activate the virulence of certain opportunistic pathogens. Here, we considered that endogenous opioids might function as such compounds, ...
    • Evidence for Direct Control of Virulence and Defense Gene Circuits by the Pseudomonas aeruginosa Quorum Sensing Regulator, MvfR 

      Maura, Damien; Hazan, Ronen; Kitao, Tomoe; Ballok, Alicia E.; Rahme, Laurence G. (Nature Publishing Group, 2016)
      Pseudomonas aeruginosa defies eradication by antibiotics and is responsible for acute and chronic human infections due to a wide variety of virulence factors. Currently, it is believed that MvfR (PqsR) controls the expression ...
    • Homeostatic Interplay between Bacterial Cell-Cell Signaling and Iron in Virulence 

      Hazan, Ronen N.; He, Jianxin; Xiao, Gaoping; Dekimpe, Valérie; Apidianakis, Yiorgos; Lesic, Biliana; Astrakas, Christos; Déziel, Eric; Lépine, François; Rahme, Laurence G. (Public Library of Science, 2010)
      Pathogenic bacteria use interconnected multi-layered regulatory networks, such as quorum sensing (QS) networks to sense and respond to environmental cues and external and internal bacterial cell signals, and thereby adapt ...
    • Honey’s Ability to Counter Bacterial Infections Arises from Both Bactericidal Compounds and QS Inhibition 

      Wang, Rui; Starkey, Melissa; Hazan, Ronen N.; Rahme, Laurence G. (Frontiers Research Foundation, 2012)
      The ability of honey to kill bacterial pathogens in vitro and quickly clear even chronic or drug-resistant infections has been demonstrated by several studies. Most current research is focused on identifying the bactericidal ...
    • Identification of Anti-virulence Compounds That Disrupt Quorum-Sensing Regulated Acute and Persistent Pathogenicity 

      Starkey, Melissa; Lepine, Francois; Maura, Damien; Bandyopadhaya, Arunava; Lesic, Biljana; He, Jianxin; Kitao, Tomoe; Righi, Valeria; Milot, Sylvain; Tzika, Aria; Rahme, Laurence (Public Library of Science, 2014)
      Etiological agents of acute, persistent, or relapsing clinical infections are often refractory to antibiotics due to multidrug resistance and/or antibiotic tolerance. Pseudomonas aeruginosa is an opportunistic Gram-negative ...
    • Inhibitors of Pathogen Intercellular Signals as Selective Anti-Infective Compounds 

      Lesic, Biliana; Lépine, François; Déziel, Eric; Zhang, Qunhao; Padfield, Katie; Castonguay, Marie-Hélène; Milot, Sylvain; Stachel, Scott; Zhang, Jiangwen; Tzika, A. Aria; Tompkins, Ronald Gary; Rahme, Laurence G. (Public Library of Science, 2007)
      Long-term antibiotic use generates pan-resistant super pathogens. Anti-infective compounds that selectively disrupt virulence pathways without affecting cell viability may be used to efficiently combat infections caused ...
    • Involvement of Skeletal Muscle Gene Regulatory Network in Susceptibility to Wound Infection Following Trauma 

      Apidianakis, Yiorgos; Mindrinos, Michael N.; Xiao, Wenzhong; Tegos, George; Papisov, Mikhail Ivanovich; Hamblin, Michael; Davis, Ronald W.; Tompkins, Ronald Gary; Rahme, Laurence G. (Public Library of Science, 2007)
      Despite recent advances in our understanding the pathophysiology of trauma, the basis of the predisposition of trauma patients to infection remains unclear. A Drosophila melanogaster/Pseudomonas aeruginosa injury and ...
    • A Method for High Throughput Determination of Viable Bacteria Cell Counts in 96-Well Plates 

      Hazan, Ronen N.; Que, Yok-Ai; Maura, Damien; Rahme, Laurence G. (BioMed Central, 2012)
      Background: There are several methods for quantitating bacterial cells, each with advantages and disadvantages. The most common method is bacterial plating, which has the advantage of allowing live cell assessment through ...
    • Nuclear magnetic resonance in conjunction with functional genomics suggests mitochondrial dysfunction in a murine model of cancer cachexia 

      CONSTANTINOU, CATERINA; FONTES DE OLIVEIRA, CIBELY CRISTINE; MINTZOPOULOS, DIONYSSIOS; BUSQUETS, SILVIA; HE, JIANXIN; KESARWANI, MEENU; MINDRINOS, MICHAEL; RAHME, LAURENCE G.; ARGILÉS, JOSEP M.; TZIKA, A. ARIA (D.A. Spandidos, 2011)
      Cancer patients commonly suffer from cachexia, a syndrome in which tumors induce metabolic changes in the host that lead to massive loss in skeletal muscle mass. Using a preclinical mouse model of cancer cachexia, we tested ...
    • The Pathogenic Properties of a Novel and Conserved Gene Product, KerV, in Proteobacteria 

      An, Dingding; Apidianakis, Yiorgos; Boechat, Ana Laura; Baldini, Regina L.; Goumnerov, Boyan C.; Rahme, Laurence G. (Public Library of Science, 2009)
      Identification of novel virulence factors is essential for understanding bacterial pathogenesis and designing antibacterial strategies. In this study, we uncover such a factor, termed KerV, in Proteobacteria. Experiments ...
    • Prediction of Multiple Infections After Severe Burn Trauma 

      Yan, Shuangchun; Tsurumi, Amy; Que, Yok-Ai; Ryan, Colleen M.; Bandyopadhaya, Arunava; Morgan, Alexander A.; Flaherty, Patrick J.; Tompkins, Ronald Gary; Rahme, Laurence G. (Ovid Technologies (Wolters Kluwer Health), 2015)
      OBJECTIVE: To develop predictive models for early triage of burn patients based on hypersusceptibility to repeated infections. BACKGROUND: Infection remains a major cause of mortality and morbidity after severe trauma, ...
    • Production of Pseudomonas aeruginosa Intercellular Small Signaling Molecules in Human Burn Wounds 

      Que, Yok-Ai; Hazan, Ronen N.; Ryan, Colleen M.; Milot, Sylvain; Lépine, François; Lydon, Martha; Rahme, Laurence G. (SAGE-Hindawi Access to Research, 2011)
      Pseudomonas aeruginosa has developed a complex cell-to-cell communication system that relies on low-molecular weight excreted molecules to control the production of its virulence factors. We previously characterized the ...
    • A Quorum Sensing Small Volatile Molecule Promotes Antibiotic Tolerance in Bacteria 

      Que, Yok-Ai; Hazan, Ronen; Strobel, Benjamin; Maura, Damien; He, Jianxin; Kesarwani, Meenu; Panopoulos, Panagiotis; Tsurumi, Amy; Giddey, Marlyse; Wilhelmy, Julie; Mindrinos, Michael N.; Rahme, Laurence G. (Public Library of Science, 2013)
      Bacteria can be refractory to antibiotics due to a sub-population of dormant cells, called persisters that are highly tolerant to antibiotic exposure. The low frequency and transience of the antibiotic tolerant “persister” ...
    • The Quorum Sensing Volatile Molecule 2-Amino Acetophenon Modulates Host Immune Responses in a Manner that Promotes Life with Unwanted Guests 

      Bandyopadhaya, Arunava; Kesarwani, Meenu; Que, Yok-Ai; He, Jianxin; Padfield, Katie; Tompkins, Ronald Gary; Rahme, Laurence G. (Public Library of Science, 2012)
      Increasing evidence indicates that bacterial quorum sensing (QS) signals are important mediators of immunomodulation. However, whether microbes utilize these immunomodulatory signals to maintain infection remain unclear. ...
    • A Small Volatile Bacterial Molecule Triggers Mitochondrial Dysfunction in Murine Skeletal Muscle 

      Tzika, A. Aria; Constantinou, Caterina; Bandyopadhaya, Arunava; Psychogios, Nikolaos; Lee, Sangseok; Mindrinos, Michael; Martyn, J. A. Jeevendra; Tompkins, Ronald G.; Rahme, Laurence G. (Public Library of Science, 2013)
      Mitochondria integrate distinct signals that reflect specific threats to the host, including infection, tissue damage, and metabolic dysfunction; and play a key role in insulin resistance. We have found that the Pseudomonas ...
    • TNF-α/IL-10 Ratio Correlates with Burn Severity and May Serve as a Risk Predictor of Increased Susceptibility to Infections 

      Tsurumi, Amy; Que, Yok-Ai; Ryan, Colleen M.; Tompkins, Ronald G.; Rahme, Laurence G. (Frontiers Media S.A., 2016)
      Severe burn injury renders patients susceptible to multiple infection episodes; however, identifying specific patient groups at high risk remains challenging. Burn-induced inflammatory response dramatically modifies the ...
    • Use of the Lambda Red Recombinase System to Rapidly Generate Mutants in Pseudomonas aeruginosa 

      Lesic, Biliana; Rahme, Laurence G. (BioMed Central, 2008)
      Background: The Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in various bacteria and fungi. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment ...