Browsing by Author "Mosberg, Joshua Adam Weintrob"
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Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases
Mosberg, Joshua Adam Weintrob; Gregg, Christopher; Lajoie, Marc Joseph; Wang, Harris He; Church, George McDonald (Public Library of Science, 2012)Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous ... -
Manipulating Replisome Dynamics to Enhance Lambda Red-Mediated Multiplex Genome Engineering
Lajoie, Marc Joseph; Gregg, Christopher; Mosberg, Joshua Adam Weintrob; Washington, G. C.; Church, George McDonald (Oxford University Press, 2012)Disrupting the interaction between primase and helicase in Escherichia coli increases Okazaki fragment (OF) length due to less frequent primer synthesis. We exploited this feature to increase the amount of ssDNA at the ... -
Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli
Mosberg, Joshua Adam Weintrob (2013-10-08)The phage-derived Lambda Red recombination system utilizes exogenous DNA in order to generate precise insertion, deletion, and point mutations in Escherichia coli and other bacteria. Due to its convenience, it is a ...