Now showing items 1-2 of 2

    • All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins 

      Hochbaum, Daniel R.; Zhao, Yongxin; Farhi, Samouil L.; Klapoetke, Nathan; Werley, Christopher A.; Kapoor, Vikrant; Zou, Peng; Kralj, Joel M.; Maclaurin, Dougal; Smedemark-Margulies, Niklas; Saulnier, Jessica L.; Boulting, Gabriella L.; Straub, Christoph; Cho, Yong Ku; Melkonian, Michael; Wong, Gane Ka-Shu; Harrison, D. Jed; Murthy, Venkatesh N.; Sabatini, Bernardo; Boyden, Edward S.; Campbell, Robert E.; Cohen, Adam E. (2014)
      All-optical electrophysiology—spatially resolved simultaneous optical perturbation and measurement of membrane voltage—would open new vistas in neuroscience research. We evolved two archaerhodopsin-based voltage indicators, ...
    • CRISPR/Cas9-Mediated Gene Knock-Down in Post-Mitotic Neurons 

      Straub, Christoph; Granger, Adam J.; Saulnier, Jessica L.; Sabatini, Bernardo L. (Public Library of Science, 2014)
      The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for genome editing in eukaryotic cells. This technique allows for sequence-specific induction of double-strand breaks in genomic DNA of individual ...