Now showing items 1-20 of 32

    • Analysis of Salmonella enterica Serotype Paratyphi A Gene Expression in the Blood of Bacteremic Patients in Bangladesh 

      Sheikh, Alaullah; Bhuiyan, Md. Saruar; Khanam, Farhana; Bukka, Archana; Kalsy, Anuj; Porwollik, Steffen; Cravioto, Alejandro; Logvinenko, Tanya; McClelland, Michael; Graham, James E.; Qadri, Firdausi; Charles, Richelle Candice; Rollins, Sean McKenzie; Harris, Jason B.; Brooks, W. Abdullah; Larocque, Regina Celes; Hohmann, Elizabeth Louise; Calderwood, Stephen Beaven; Ryan, Edward Thomas (Public Library of Science, 2010)
      Background: Salmonella enterica serotype Paratyphi A is a human-restricted cause of paratyphoid fever, accounting for up to a fifth of all cases of enteric fever in Asia. Methodology/Principal Findings: In this work, we ...
    • Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults 

      Alam, Mohammad Murshid; Aktar, Amena; Afrin, Sadia; Rahman, Mohammad Arif; Aktar, Sarmin; Uddin, Taher; Rahman, M. Arifur; Mahbuba, Deena Al; Chowdhury, Fahima; Khan, Ashraful Islam; Bhuiyan, Taufiqur Rahman; Begum, Yasmin Ara; Ryan, Edward T.; Calderwood, Stephen B.; Svennerholm, Ann-Mari; Qadri, Firdausi (Public Library of Science, 2014)
      Background: Multiple infections with diverse enterotoxigenic E. coli (ETEC) strains lead to broad spectrum protection against ETEC diarrhea. However, the precise mechanism of protection against ETEC infection is still ...
    • Application of In Vivo Induced Antigen Technology (IVIAT) to Bacillus anthracis 

      Peppercorn, Amanda; Young, John S.; Drysdale, Melissa; Baresch, Andrea; Bikowski, Margaret V.; Ashford, David A.; Quinn, Conrad P.; Handfield, Martin; Hillman, Jeffrey D.; Lyons, C. Rick; Koehler, Theresa M.; Sonenshein, Abraham L.; Rollins, Sean McKenzie; Calderwood, Stephen Beaven; Ryan, Edward Thomas (Public Library of Science, 2008)
      In vivo induced antigen technology (IVIAT) is an immuno-screening technique that identifies bacterial antigens expressed during infection and not during standard in vitro culturing conditions. We applied IVIAT to Bacillus ...
    • Biomarkers of Environmental Enteropathy are Positively Associated with Immune Responses to an Oral Cholera Vaccine in Bangladeshi Children 

      Uddin, Muhammad Ikhtear; Islam, Shahidul; Nishat, Naoshin S.; Hossain, Motaher; Rafique, Tanzeem Ahmed; Rashu, Rasheduzzaman; Hoq, Mohammad Rubel; Zhang, Yue; Saha, Amit; Harris, Jason B.; Calderwood, Stephen B.; Bhuiyan, Taufiqur Rahman; Ryan, Edward T.; Leung, Daniel T.; Qadri, Firdausi (Public Library of Science, 2016)
      Environmental enteropathy (EE) is a poorly understood condition that refers to chronic alterations in intestinal permeability, absorption, and inflammation, which mainly affects young children in resource-limited settings. ...
    • Cellular and Cytokine Responses to Salmonella enterica Serotype Typhi Proteins in Patients with Typhoid Fever in Bangladesh 

      Bhuiyan, Saruar; Sayeed, Abu; Khanam, Farhana; Leung, Daniel T.; Rahman Bhuiyan, Taufiqur; Sheikh, Alaullah; Salma, Umme; LaRocque, Regina C.; Harris, Jason B.; Pacek, Marcin; Calderwood, Stephen B.; LaBaer, Joshua; Ryan, Edward T.; Qadri, Firdausi; Charles, Richelle C. (The American Society of Tropical Medicine and Hygiene, 2014)
      We assessed interferon-gamma (IFN-γ) responses via enzyme-linked immunosorbent spot (ELISPOT) to a number of S. Typhi antigens in samples from humans with S. Typhi bacteremia and typhoid fever in Bangladesh. Compared with ...
    • A Cholera Conjugate Vaccine Containing O-specific Polysaccharide (OSP) of V. cholerae O1 Inaba and Recombinant Fragment of Tetanus Toxin Heavy Chain (OSP:rTTHc) Induces Serum, Memory and Lamina Proprial Responses against OSP and Is Protective in Mice 

      Sayeed, Md. Abu; Bufano, Meagan Kelly; Xu, Peng; Eckhoff, Grace; Charles, Richelle C.; Alam, Mohammad Murshid; Sultana, Tania; Rashu, Md. Rasheduzzaman; Berger, Amanda; Gonzalez-Escobedo, Geoffrey; Mandlik, Anjali; Bhuiyan, Taufiqur Rahman; Leung, Daniel T.; LaRocque, Regina C.; Harris, Jason B.; Calderwood, Stephen B.; Qadri, Firdausi; Vann, W. F.; Kováč, Pavol; Ryan, Edward T. (Public Library of Science, 2015)
      Background: Vibrio cholerae is the cause of cholera, a severe watery diarrhea. Protection against cholera is serogroup specific. Serogroup specificity is defined by the O-specific polysaccharide (OSP) component of ...
    • Circulating Mucosal Associated Invariant T Cells Are Activated in Vibrio cholerae O1 Infection and Associated with Lipopolysaccharide Antibody Responses 

      Leung, Daniel T.; Bhuiyan, Taufiqur R.; Nishat, Naoshin S.; Hoq, Mohammad Rubel; Aktar, Amena; Rahman, M. Arifur; Uddin, Taher; Khan, Ashraful I.; Chowdhury, Fahima; Charles, Richelle C.; Harris, Jason B.; Calderwood, Stephen B.; Qadri, Firdausi; Ryan, Edward T. (Public Library of Science, 2014)
      Background: Mucosal Associated Invariant T (MAIT) cells are innate-like T cells found in abundance in the intestinal mucosa, and are thought to play a role in bridging the innate-adaptive interface. Methods: We measured ...
    • Comparative Proteomic Analysis of the PhoP Regulon in Salmonella enterica Serovar Typhi Versus Typhimurium 

      Lebrun, Lauren M.; Sheikh, Alaullah; Logvinenko, Tanya; Tarique, Abdullah; Krastins, Bryan; Qadri, Firdausi; Charles, Richelle Candice; Harris, Jason B.; Chase, Michael Richard; Larocque, Regina Celes; Rollins, Sean McKenzie; Hohmann, Elizabeth Louise; Rosenberg, Ian Morley; Sarracino, David A.; Calderwood, Stephen Beaven; Ryan, Edward Thomas (Public Library of Science, 2009)
      Background: S. Typhi, a human-restricted Salmonella enterica serovar, causes a systemic intracellular infection in humans (typhoid fever). In comparison, S. Typhimurium causes gastroenteritis in humans, but causes a systemic ...
    • Contribution of Gelatinase, Serine Protease, and fsr to the Pathogenesis of Enterococcus faecalis Endophthalmitis 

      Engelbert, M.; Mylonakis, E; Ausubel, Frederick M.; Calderwood, Stephen Beaven; Gilmore, Michael S. (American Society for Microbiology, 2004)
      Gelatinase and serine protease were found to contribute in concert to pathogenesis in a rabbit model of endophthalmitis. However, a mutant defective in the fsr regulator was observed to be more attenuated than a mutant ...
    • The Enterococcus faecalis fsrB Gene, a Key Component of the fsr Quorum-Sensing System, Is Associated with Virulence in the Rabbit Endophthalmitis Model 

      Mylonakis, E.; Engelbert, M.; Qin, X.; Sifri, C. D.; Murray, B. E.; Ausubel, Frederick M.; Gilmore, Michael S.; Calderwood, Stephen Beaven (American Society for Microbiology, 2002)
      We used a rabbit endophthalmitis model to explore the role of fsrB, a gene required for the function of the fsr quorum-sensing system of Enterococcus faecalis, in pathogenicity. A nonpolar deletion mutant of fsrB had ...
    • Evaluation in Mice of a Conjugate Vaccine for Cholera Made from Vibrio cholerae O1 (Ogawa) O-Specific Polysaccharide 

      Alam, Mohammad Murshid; Bufano, Megan Kelly; Xu, Peng; Kalsy, Anuj; Yu, Y.; Freeman, Y. Wu; Sultana, Tania; Rashu, Md. Rasheduzzaman; Desai, Ishaan; Eckhoff, Grace; Leung, Daniel T.; Charles, Richelle C.; LaRocque, Regina C.; Harris, Jason B.; Clements, John D.; Calderwood, Stephen B.; Qadri, Firdausi; Vann, W. F.; Kováč, Pavol; Ryan, Edward T. (Public Library of Science, 2014)
      Background: Protective immunity against cholera is serogroup specific. Serogroup specificity in Vibrio cholerae is determined by the O-specific polysaccharide (OSP) of lipopolysaccharide (LPS). Generally, polysaccharides ...
    • Evaluation of a Typhoid/Paratyphoid Diagnostic Assay (TPTest) Detecting Anti-Salmonella IgA in Secretions of Peripheral Blood Lymphocytes in Patients in Dhaka, Bangladesh 

      Khanam, Farhana; Sheikh, Alaullah; Sayeed, Md. Abu; Bhuiyan, Md. Saruar; Choudhury, Feroza Kaneez; Salma, Umme; Pervin, Shahnaz; Sultana, Tania; Ahmed, Dilruba; Goswami, Doli; Hossain, Md. Lokman; Mamun, K. Z.; Charles, Richelle C.; Brooks, W. Abdullah; Calderwood, Stephen B.; Cravioto, Alejandro; Ryan, Edward T.; Qadri, Firdausi (Public Library of Science, 2013)
      Background: Rapid and reliable diagnostic assays for enteric (typhoid and paratyphoid) fever are urgently needed. We report the characterization of novel approach utilizing lymphocyte secretions, for diagnosing patients ...
    • Evolutionarily Conserved Recognition and Innate Immunity to Fungal Pathogens by the Scavenger Receptors SCARF1 and CD36 

      Tampakakis, Emmanouil; Puckett, Lindsay; Tai, Melissa F.; Stewart, Cameron R.; Hickman, Suzanne E.; Moore, Kathryn J.; El Khoury, Joseph; Means, Terry K.; Mylonakis, Eleftherios; Colvin, Richard Anthony; Seung, Edward N.; Pukkila-Worley, Read; Calderwood, Stephen Beaven; Hacohen, Nir; Luster, Andrew David (Rockefeller University Press, 2009)
      Receptors involved in innate immunity to fungal pathogens have not been fully elucidated. We show that the Caenorhabditis elegans receptors CED-1 and C03F11.3, and their mammalian orthologues, the scavenger receptors SCARF1 ...
    • High Depth, Whole-Genome Sequencing of Cholera Isolates from Haiti and the Dominican Republic 

      Sealfon, Rachel; Gire, Stephen; Ellis, Crystal Nicole; Calderwood, Stephen Beaven; Qadri, Firdausi; Hensley, Lisa; Kellis, Manolis; Ryan, Edward Thomas; Larocque, Regina Celes; Harris, Jason B.; Sabeti, Pardis Christine (BioMed Central, 2012)
      Background: Whole-genome sequencing is an important tool for understanding microbial evolution and identifying the emergence of functionally important variants over the course of epidemics. In October 2010, a severe cholera ...
    • Household Transmission of Vibrio cholerae in Bangladesh 

      Sugimoto, Jonathan D.; Koepke, Amanda A.; Kenah, Eben E.; Halloran, M. Elizabeth; Chowdhury, Fahima; Khan, Ashraful I.; LaRocque, Regina C.; Yang, Yang; Ryan, Edward T.; Qadri, Firdausi; Calderwood, Stephen B.; Harris, Jason B.; Longini, Ira M. (Public Library of Science, 2014)
      Background: Vibrio cholerae infections cluster in households. This study's objective was to quantify the relative contribution of direct, within-household exposure (for example, via contamination of household food, water, ...
    • Identification of Immunogenic Salmonella enterica Serotype Typhi Antigens Expressed in Chronic Biliary Carriers of S. Typhi in Kathmandu, Nepal 

      Charles, Richelle C.; Sultana, Tania; Alam, Mohammad Murshid; Yu, Yanan; Wu-Freeman, Ying; Bufano, Meagan Kelly; Rollins, Sean M.; Tsai, Lillian; Harris, Jason B.; LaRocque, Regina C.; Leung, Daniel T.; Brooks, W. Abdullah; Nga, Tran Vu Thieu; Dongol, Sabina; Basnyat, Buddha; Calderwood, Stephen B.; Farrar, Jeremy; Khanam, Farhana; Gunn, John S.; Qadri, Firdausi; Baker, Stephen; Ryan, Edward T. (Public Library of Science, 2013)
      Background: Salmonella enterica serotype Typhi can colonize and persist in the biliary tract of infected individuals, resulting in a state of asymptomatic chronic carriage. Chronic carriers may act as persistent reservoirs ...
    • Immunogenicity of a Killed Bivalent (O1 and O139) Whole Cell Oral Cholera Vaccine, Shanchol, in Haiti 

      Charles, Richelle C.; Hilaire, Isabelle J.; Mayo-Smith, Leslie M.; Teng, Jessica E.; Jerome, J. Gregory; Franke, Molly F.; Saha, Amit; Yu, Yanan; Kováč, Paul; Calderwood, Stephen B.; Ryan, Edward T.; LaRocque, Regina C.; Almazor, Charles P.; Qadri, Firdausi; Ivers, Louise C.; Harris, Jason B. (Public Library of Science, 2014)
      Background: Studies of the immunogenicity of the killed bivalent whole cell oral cholera vaccine, Shanchol, have been performed in historically cholera-endemic areas of Asia. There is a need to assess the immunogenicity ...
    • Immunologic Responses to Vibrio cholerae in Patients Co-Infected with Intestinal Parasites in Bangladesh 

      Bhuiyan, Taufiqur R.; Chowdhury, Fahima; Khan, Ashraful I.; Logvinenko, Tanya; Kendall, Jennifer; Faruque, Abu S. G.; Nagler, Cathryn R.; Qadri, Firdausi; Harris, Jason B.; Podolsky, Michael Jeffrey; Larocque, Regina Celes; Ryan, Edward Thomas; Calderwood, Stephen Beaven (Public Library of Science, 2009)
      Background: Infection with intestinal helminths is common and may contribute to the decreased efficacy of Vibrio cholerae vaccines in endemic compared to non-endemic areas. However, the immunomodulatory effects of concomitant ...
    • Individuals with Le(a+b−) Blood Group have Increased Susceptibility to Symptomatic \(Vibrio\) \(cholerae\) O1 Infection 

      Arifuzzaman, Mohammad; Ahmed, Tanvir; Chowdhury, Fahima; Rashu, Rasheduzzaman; Khan, Ashraful I.; Bhuiyan, Taufiqur Rahman; Qadri, Firdausi; Rahman, Mohammad Arif; Larocque, Regina Celes; Harris, Jason B.; Ryan, Edward Thomas; Calderwood, Stephen Beaven (Public Library of Science, 2011)
      Background: Human genetic factors such as blood group antigens may affect the severity of infectious diseases. Presence of specific ABO and Lewis blood group antigens has been shown previously to be associated with the ...
    • A magneto-DNA nanoparticle system for the rapid and sensitive diagnosis of enteric fever 

      Park, Ki Soo; Chung, Hyun Jung; Khanam, Farhana; Lee, Hakho; Rashu, Rasheduzzaman; Bhuiyan, Md. Taufiqur; Berger, Amanda; Harris, Jason B.; Calderwood, Stephen B.; Ryan, Edward T.; Qadri, Firdausi; Weissleder, Ralph; Charles, Richelle C. (Nature Publishing Group, 2016)
      There is currently no widely available optimal assay for diagnosing patients with enteric fever. Here we present a novel assay designed to detect amplified Salmonella nucleic acid (mRNA) using magneto-DNA probes and a ...