Now showing items 1-20 of 26

    • Brain Swelling and Death in Children with Cerebral Malaria 

      Seydel, Karl B.; Kampondeni, Samuel D.; Valim, Clarissa; Potchen, Michael J.; Milner, Danny Arnold; Muwalo, Francis W.; Birbeck, Gretchen L.; Bradley, William; Fox, Lindsay L.; Glover, Simon J.; Hammond, Colleen A.; Heyderman, Robert S.; Chilingulo, Cowles A.; Molyneux, Malcolm E.; Taylor, Terrie E. (New England Journal of Medicine (NEJM/MMS), 2015)
      BACKGROUND: Case fatality rates among African children with cerebral malaria remain in the range of 15 to 25%. The key pathogenetic processes and causes of death are unknown, but a combination of clinical observations and ...
    • Confirmation of emergence of mutations associated with atovaquone-proguanil resistance in unexposed Plasmodium falciparum isolates from Africa 

      Happi, Christian Tientcha; Gbotosho, Grace O; Folarin, Onikepe A; Milner, Danny Arnold; Sarr, Ousmane; Sowunmi, Akintunde; Kyle, Dennis E; Milhous, Wilbur K; Wirth, Dyann Fergus; Oduola, Ayoade MJ (BioMed Central, 2006)
      Background: In vitro and in vivo resistance of Plasmodium falciparum to atovaquone or atovaquone-proguanil hydrochloride combination has been associated to two point mutations in the parasite cytochrome b (cytb) gene ...
    • Differential PfEMP1 Expression Is Associated with Cerebral Malaria Pathology 

      Tembo, Dumizulu L.; Nyoni, Benjamin; Murikoli, Rekah V.; Mukaka, Mavuto; Milner, Danny A.; Berriman, Matthew; Rogerson, Stephen J.; Taylor, Terrie E.; Molyneux, Malcolm E.; Mandala, Wilson L.; Craig, Alister G.; Montgomery, Jacqui (Public Library of Science, 2014)
      Plasmodium falciparum is unique among human malarias in its ability to sequester in post-capillary venules of host organs. The main variant antigens implicated are the P. falciparum erythrocyte membrane protein 1 (PfEMP1), ...
    • Discordance between clinical and immunological ART eligibility criteria for children in Malawi 

      O’Hare, Bernadette; Milner, Danny A; Newberry, Laura; Pelani, Isaac; Malisita, Ken (BioMed Central, 2014)
      Background: Since May 2014, all HIV positive children aged less than five years in Malawi are eligible for ART. For children older than five years they are eligible if they are in WHO stage III/IV, if stage I/II, if their ...
    • Evidence for spleen dysfunction in malaria-HIV co-infection in a subset of pediatric patients 

      Joice, Regina; Frantzreb, Charles; Pradham, Alana; Seydel, Karl B.; Kamiza, Steve; Wirth, Dyann F.; Duraisingh, Manoj T.; Molyneux, Malcolm E; Taylor, Terrie E.; Marti, Matthias; Milner, Danny A. (2015)
      The spleen has an important role in the clearance of malaria parasites, and the role of HIV co-infection on this process is yet to be described. Using a combination of histological and molecular methods, we systematically ...
    • Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection 

      Hochman, Sarah E.; Madaline, Theresa F.; Wassmer, Samuel C.; Mbale, Emmie; Choi, Namjong; Seydel, Karl B.; Whitten, Richard O.; Varughese, Julie; Grau, Georges E. R.; Kamiza, Steve; Molyneux, Malcolm E.; Taylor, Terrie E.; Lee, Sunhee; Milner, Danny A.; Kim, Kami (American Society of Microbiology, 2015)
      ABSTRACT Cerebral malaria (CM) is a major contributor to malaria deaths, but its pathophysiology is not well understood. While sequestration of parasitized erythrocytes is thought to be critical, the roles of inflammation ...
    • A General SNP-Based Molecular Barcode for Plasmodium Falciparum Identification and Tracking 

      Mahesh, Nira; Rosen, David; Daniels, Rachel; Volkman, Sarah K.; Milner, Danny Arnold; Neafsey, Daniel Edward; Park, Daniel John; Angelino, Elaine; Sabeti, Pardis Christine; Wirth, Dyann Fergus; Wiegand, Roger C. (BioMed Central, 2008)
      Background: Single nucleotide polymorphism (SNP) genotyping provides the means to develop a practical, rapid, inexpensive assay that will uniquely identify any Plasmodium falciparum parasite using a small amount of DNA. ...
    • Genome-Wide SNP Genotyping Highlights the Role of Natural Selection in Plasmodium Falciparum Population Divergence 

      Wirth, Dyann F.; Sabeti, Pardis C.; Lander, Eric S.; Birren, Bruce W.; Hartl, Daniel; Wiegand, Roger; Chitnis, Chetan E.; Dash, Aditya P.; do Lago Moraes, Sandra; Ferreira, Marcelo U.; Mboup, Soulyemane; Ndir, Omar; Ndiaye, Daouda; Ousmane, Sarr; Stange-Thomann, Nicole; Gates, Casey; Tyndall, Erin; Cortese, Joseph F.; Houde, Nathan; Daniels, Rachel; Rosen, David; Lukens, Amanda; Milner, Danny; Montgomery, Philip; Park, Daniel; Volkman, Sarah K.; Schaffner, Stephen F.; Neafsey, Daniel (BioMed Central, 2008)
      Background: The malaria parasite Plasmodium falciparum exhibits abundant genetic diversity, and this diversity is key to its success as a pathogen. Previous efforts to study genetic diversity in P. falciparum have begun ...
    • Green Herring Syndrome: Bacterial Infection in Patients With Mucormycosis Cavitary Lung Disease 

      Peixoto, Driele; Hammond, Sarah P.; Issa, Nicolas C.; Madan, Rachna; Gill, Ritu R.; Milner, Danny A.; Colson, Yolonda L.; Koo, Sophia; Baden, Lindsey R.; Marty, Francisco M. (Oxford University Press, 2014)
      Mucormycosis is a life-threatening fungal disease in patients with hematological malignancies. The diagnosis of pulmonary mucormycosis is particularly challenging. We describe 3 mucormycosis cases with an uncommon presentation ...
    • A histological method for quantifying Plasmodium falciparum in the brain in fatal paediatric cerebral malaria 

      Milner, Danny A; Valim, Clarissa; Carr, Richard A; Chandak, Pankaj B; Fosiko, Nedson G; Whitten, Richard; Playforth, Krupa B; Seydel, Karl B; Kamiza, Steve; Molyneux, Malcolm E; Taylor, Terrie E (BioMed Central, 2013)
      Background: The sequestration of Plasmodium falciparum-infected erythrocytes in brain microvasculature through cytoadherence to endothelium, is the hallmark of the definitive diagnosis of cerebral malaria and plays a ...
    • HIV-2 Encephalitis: Case Report and Literature Review 

      Wood, Brian R.; Klein, Joshua Peter; Lyons, Jennifer L; Milner, Danny Arnold; Phillips, Richard; Schutten, Martin; Folkerth, Rebecca Dunn; Ciarlini, Pedro; Henrich, Timothy Jensen; Johnson, Jennifer Ann (Mary Ann Liebert, 2012)
      We report the case of a 59-year-old man who moved from Cape Verde to Massachusetts at the age of 29. He had multiple sexual contacts with female partners in Cape Verde and with West African women in Massachusetts, as well ...
    • Human Cerebral Malaria and Plasmodium falciparum Genotypes in Malawi 

      Milner, Danny Arnold; Vareta, Jimmy; Valim, Clarissa; Montgomery, Jacqui; Daniels, Rachel Fath; Cooke, Sarah Volkman; Neafsey, Daniel Edward; Park, Daniel John; Schaffner, Stephen; Mahesh, Nira C; Barnes, Kayla G; Rosen, David M; Lukens, Amanda Kathleen; Van-Tyne, Daria; Wiegand, Roger C.; Sabeti, Pardis Christine; Seydel, Karl B; Glover, Simon J; Kamiza, Steve; Molyneux, Malcolm E; Taylor, Terrie E; Wirth, Dyann Fergus (BioMed Central, 2012)
      Background: Cerebral malaria, a severe form of Plasmodium falciparum infection, is an important cause of mortality in sub-Saharan African children. A Taqman 24 Single Nucleotide Polymorphisms (SNP) molecular barcode assay ...
    • Identification and Functional Validation of the Novel Antimalarial Resistance Locus PF10_0355 in Plasmodium falciparum 

      Van Tyne, Daria Natalie; Park, Daniel John; Schaffner, Stephen; Neafsey, Daniel Edward; Angelino, Elaine Lee; Cortese, Joseph F.; Barnes, Kayla G.; Rosen, David M.; Lukens, Amanda Kathleen; Daniels, Rachel Fath; Milner, Danny Arnold; Johnson, Charles A.; Shlyakhter, Ilya; Grossman, Sharon Rachel; Becker, Justin S.; Yamins, Daniel Louis Kanef; Karlsson, Elinor Kathryn; Ndiaye, Daouda; Sarr, Ousmane; Mboup, Souleymane; Happi, Christian Tientcha; Furlotte, Nicholas A.; Eskin, Eleazar; Kang, Hyun Min; Hartl, Daniel L.; Birren, Bruce W.; Wiegand, Roger C.; Lander, Eric Steven; Wirth, Dyann Fergus; Cooke, Sarah Volkman; Sabeti, Pardis Christine (Public Library of Science, 2011)
      The Plasmodium falciparum parasite's ability to adapt to environmental pressures, such as the human immune system and antimalarial drugs, makes malaria an enduring burden to public health. Understanding the genetic basis ...
    • If It’s Not One Thing, It’s Another: An Inverse Relationship of Malignancy and Atherosclerotic Disease 

      Li, Matthew; Cima, Michael J; Milner, Danny A. (Public Library of Science, 2015)
      Atherosclerosis and malignancy are pervasive pathological conditions that account for the bulk of morbidity and mortality in developed countries. Our current understanding of the patholobiology of these fundamental disorders ...
    • Inferring Developmental Stage Composition from Gene Expression in Human Malaria 

      Joice, Regina; Narasimhan, Vagheesh; Montgomery, Jacqui; Sidhu, Amar Bir; Oh, Keunyoung; Meyer, Evan; Pierre-Louis, Willythssa; Seydel, Karl; Milner, Danny; Williamson, Kim; Wiegand, Roger; Ndiaye, Daouda; Daily, Johanna; Wirth, Dyann; Taylor, Terrie; Huttenhower, Curtis; Marti, Matthias (Public Library of Science, 2013)
      In the current era of malaria eradication, reducing transmission is critical. Assessment of transmissibility requires tools that can accurately identify the various developmental stages of the malaria parasite, particularly ...
    • Maternal–Fetal Microtransfusions and HIV-1 Mother-to-Child Transmission in Malawi 

      Kwiek, Jesse J.; Mwapasa, Victor; Milner, Danny Arnold; Alker, Alisa P.; Miller, William C.; Tadesse, Eyob; Molyneux, Malcolm E.; Rogerson, Stephen J.; Meshnick, Steven R. (Public Library of Science, 2006)
      Background: Between 25% and 35% of infants born to HIV-infected mothers become HIV-1 infected. One potential route of mother-to-child transmission (MTCT) could be through a breakdown in the placental barrier (i.e., ...
    • Modeling malaria genomics reveals transmission decline and rebound in Senegal 

      Daniels, Rachel Fath; Schaffner, Stephen; Wenger, Edward A.; Proctor, Joshua L.; Chang, Hsiao-Han; Wong, Wesley Philip; Baro, Nicholas; Ndiaye, Daouda; Fall, Fatou Ba; Ndiop, Medoune; Ba, Mady; Milner, Danny Arnold; Taylor, Terrie E.; Neafsey, Daniel Edward; Cooke, Sarah K.; Eckhoff, Philip A.; Hartl, Daniel L.; Wirth, Dyann F. (Proceedings of the National Academy of Sciences, 2015)
      To study the effects of malaria-control interventions on parasite population genomics, we examined a set of 1,007 samples of the malaria parasite Plasmodium falciparum collected in Thiès, Senegal between 2006 and 2013. The ...
    • Molecular Methods To Improve Diagnosis and Identification of Mucormycosis 

      Hammond, Sarah Page; Bialek, Ralf; Milner, Danny Arnold; Petschnigg, Eva M.; Baden, Lindsey Robert; Marty, Francisco Miguel (American Society for Microbiology, 2011)
      Mucormycosis is difficult to diagnose. Samples from suspected cases often fail to grow Mucorales in microbiologic cultures. We identified all hematologic malignancy and stem cell transplant patients diagnosed with proven ...
    • Peritoneal Tuberculosis in a Pregnant Woman from Haiti, United States 

      Ard, Kevin Lee; Chan, Brian Tan; Milner, Danny Arnold; Farmer, Paul Edward; Koenig, Serena Patricia (U.S. National Center for Infectious Diseases, 2013)
    • Plasmodium falciparum gene expression measured directly from tissue during human infection 

      Van Tyne, Daria; Tan, Yan; Daily, Johanna P; Kamiza, Steve; Seydel, Karl; Taylor, Terrie; Mesirov, Jill P; Wirth, Dyann F; Milner, Danny A (BioMed Central, 2014)
      Background: During the latter half of the natural 48-h intraerythrocytic life cycle of human Plasmodium falciparum infection, parasites sequester deep in endothelium of tissues, away from the spleen and inaccessible to ...