Now showing items 1-6 of 6

    • Cytoskeletal Mechanics of Proplatelet Maturation and Platelet Release 

      Thon, Jonathan Noah; Montalvo, Alejandro; Patel-Hett, Sunita; Devine, Matthew T.; Richardson, Jennifer L.; Ehrlicher, Allen Joseph; Larson, Mark K.; Hoffmeister, Karin Maria; Hartwig, John Henry; Italiano, Joseph E. (Rockefeller University Press, 2010)
      Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, ...
    • IL-1α induces thrombopoiesis through megakaryocyte rupture in response to acute platelet needs 

      Nishimura, Satoshi; Nagasaki, Mika; Kunishima, Shinji; Sawaguchi, Akira; Sakata, Asuka; Sakaguchi, Hiroyasu; Ohmori, Tsukasa; Manabe, Ichiro; Italiano, Joseph E.; Ryu, Tomiko; Takayama, Naoya; Komuro, Issei; Kadowaki, Takashi; Eto, Koji; Nagai, Ryozo (The Rockefeller University Press, 2015)
      Intravital visualization of thrombopoiesis revealed that formation of proplatelets, which are cytoplasmic protrusions in bone marrow megakaryocytes (MKs), is dominant in the steady state. However, it was unclear whether ...
    • The incredible journey: From megakaryocyte development to platelet formation 

      Machlus, Kellie R.; Italiano, Joseph E. (The Rockefeller University Press, 2013)
      Circulating blood platelets are specialized cells that prevent bleeding and minimize blood vessel injury. Large progenitor cells in the bone marrow called megakaryocytes (MKs) are the source of platelets. MKs release ...
    • Platelets Generated from Human Embryonic Stem Cells are Functional In Vitro and in the Microcirculation of Living Mice 

      Lu, Shi-Jiang; Li, Feng; Feng, Qiang; Kimbrel, Erin A; Hahm, Eunsil; Cho, Jaehyung; Lanza, Robert; Yin, Hong; Thon, Jonathan Noah; Wang, Wei; Italiano, Joseph E. (Nature Publishing Group, 2011)
      Platelets play an essential role in hemostasis and atherothrombosis. Owing to their short storage time, there is constant demand for this life-saving blood component. In this study, we report that it is feasible to generate ...
    • Scalable Generation of Universal Platelets from Human Induced Pluripotent Stem Cells 

      Feng, Qiang; Shabrani, Namrata; Thon, Jonathan N.; Huo, Hongguang; Thiel, Austin; Machlus, Kellie R.; Kim, Kyungho; Brooks, Julie; Li, Feng; Luo, Chenmei; Kimbrel, Erin A.; Wang, Jiwu; Kim, Kwang-Soo; Italiano, Joseph; Cho, Jaehyung; Lu, Shi-Jiang; Lanza, Robert (Elsevier, 2014)
      Summary Human induced pluripotent stem cells (iPSCs) provide a potentially replenishable source for the production of transfusable platelets. Here, we describe a method to generate megakaryocytes (MKs) and functional ...
    • T granules in human platelets function in TLR9 organization and signaling 

      Thon, Jonathan N.; Peters, Christopher G.; Machlus, Kellie R.; Aslam, Rukhsana; Rowley, Jesse; Macleod, Hannah; Devine, Matthew T.; Fuchs, Tobias A; Weyrich, Andrew S.; Semple, John W.; Flaumenhaft, Robert C.; Italiano, Joseph E. (The Rockefeller University Press, 2012)
      Human and murine platelets (PLTs) variably express toll-like receptors (TLRs), which link the innate and adaptive immune responses during infectious inflammation and atherosclerotic vascular disease. In this paper, we show ...