Now showing items 1-20 of 23

    • Cbx Proteins Help ESCs Walk the Line between Self-Renewal and Differentiation 

      Camahort, Raymond; Cowan, Chad A. (Elsevier BV, 2012)
      The Polycomb repressive complexes (PRC) regulate self-renewal and differentiation in embryonic stem cells (ESCs). In this issue of "Cell Stem Cell," Morey et al. (2012) and O'Loghlen et al. (2012) report that dynamic ...
    • A comparison of non-integrating reprogramming methods 

      Schlaeger, Thorsten M.; Daheron, Laurence Marie; Brickler, Thomas R; Entwisle, Samuel; Chan, Karrie; Cianci, Amelia; DeVine, Alexander; Ettenger, Andrew; Fitzgerald, Kelly; Godfrey, Michelle; Gupta, Dipti; McPherson, Jade; Malwadkar, Prerana; Gupta, Manav; Bell, Blair; Doi, Akiko; Jung, Namyoung; Li, Xin; Lynes, Maureen M.; Brookes, Emily; Cherry, Anne B C; Demirbas, Didem; Tsankov, Alexander; Zon, Leonard Ira; Rubin, Lee; Feinberg, Andrew P; Meissner, Alexander; Cowan, Chad A.; Daley, George Quentin (Nature Publishing Group, 2014)
      Human induced pluripotent stem cells (hiPSCs1, 2, 3) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation ...
    • Derivation of Corneal Keratocyte-Like Cells from Human Induced Pluripotent Stem Cells 

      Naylor, Richard W.; McGhee, Charles N. J.; Cowan, Chad A.; Davidson, Alan J.; Holm, Teresa M.; Sherwin, Trevor (Public Library of Science, 2016)
      Corneal diseases such as keratoconus represent a relatively common disorder in the human population. However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. Cell based ...
    • Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9 

      Mandal, Pankaj Kumar; Ferreira, Leonardo Manuel Ramos; Collins, Ryan; Meissner, Torsten B; Boutwell, C; Friesen, Max; Vrbanac, Vladimir; Garrison, Brian Scott; Stortchevoi, Alexei; Bryder, David; Musunuru, Kiran; Brand, Harrison Andrew; Tager, Andrew Martin; Allen, Todd; Talkowski, Michael Edward; Rossi, Derrick J; Cowan, Chad A. (Elsevier BV, 2014)
      Genome editing via CRISPR/Cas9 has rapidly become the tool of choice by virtue of its efficacy and ease of use. However, CRISPR/Cas9-mediated genome editing in clinically relevant human somatic cells remains untested. Here, ...
    • Enhanced Efficiency of Human Pluripotent Stem Cell Genome Editing through Replacing TALENs with CRISPRs 

      Ding, Qiurong; Regan, Stephanie Nicole; Xia, Yulei; Oostrom, Leoníe A.; Cowan, Chad A.; Musunuru, Kiran (Elsevier BV, 2013)
    • Expression Analysis of Macrodactyly Identifies Pleiotrophin Upregulation 

      Lau, Frank; Xia, Fang; Kaplan, Adam; Cerrato, Felecia; Greene, Arin K.; Taghinia, Amir Hosein; Cowan, Chad A.; Labow, Brian Ian (Public Library of Science, 2012)
      Macrodactyly is a rare family of congenital disorders characterized by the diffuse enlargement of 1 or more digits. Multiple tissue types within the affected digits are involved, but skeletal patterning and gross morphological ...
    • From Noncoding Variant to Phenotype via SORT1 at the 1p13 Cholesterol Locus 

      Musunuru, Kiran; Strong, Alanna; Frank-Kamenetsky, Maria; Lee, Noemi E.; Ahfeldt, Tim; Sachs, Katherine V.; Li, Xiaoyu; Li, Hui; Kuperwasser, Nicolas; Ruda, Vera M.; Pirruccello, James Paul; Muchmore, Brian; Prokunina-Olsson, Ludmila; Hall, Jennifer L.; Schadt, Eric E.; Morales, Carlos R.; Lund-Katz, Sissel; Phillips, Michael C.; Wong, Jamie; Cantley, William; Racie, Timothy; Ejebe, Kenechi G.; Orho-Melander, Marju; Melander, Olle; Koteliansky, Victor; Fitzgerald, Kevin; Krauss, Ronald M.; Cowan, Chad A.; Kathiresan, Sekar; Rader, Daniel J. (Springer Nature, 2010)
      Recent genome-wide association studies (GWASs) have identified a locus on chromosome 1p13 strongly associated with both plasma low-density lipoprotein cholesterol (LDL-C) and myocardial infarction (MI) in humans. Here we ...
    • Generation of Multipotent Lung and Airway Progenitors from Mouse ESCs and Patient-Specific Cystic Fibrosis iPSCs 

      Mou, Hongmei; Zhao, Rui; Sherwood, Richard Irving; Ahfeldt, Tim; Lapey, Allen; Wain, John Charles; Sicilian, Leonard; Izvolsky, Konstantin; Lau, Frank; Musunuru, Kiran; Cowan, Chad A.; Rajagopal, Jayaraj (Elsevier BV, 2012)
      Deriving lung progenitors from patient-specific pluripotent cells is a key step in producing differentiated lung epithelium for disease modeling and transplantation. By mimicking the signaling events that occur during mouse ...
    • Genome-wide Chromatin State Transitions Associated with Developmental and Environmental Cues 

      Zhu, Jiang; Adli, Mazhar; Zou, James Y.; Verstappen, Griet; Coyne, Michael J.; Zhang, Xiaolan; Durham, Timothy; Miri, Mohammad; Deshpande, Vikram; De Jager, Philip L.; Bennett, David A.; Houmard, Joseph A.; Muoio, Deborah M.; Onder, Tamer T.; Camahort, Raymond; Cowan, Chad A.; Meissner, Alexander; Epstein, Charles B.; Shoresh, Noam; Bernstein, Bradley E. (Elsevier BV, 2013)
      Differences in chromatin organization are key to the multiplicity of cell states that arise from a single genetic background, yet the landscapes of in vivo tissues remain largely uncharted. Here, we mapped chromatin ...
    • Genomewide Analysis of PRC1 and PRC2 Occupancy Identifies Two Classes of Bivalent Domains 

      Rheinbay, Esther; Endoh, Mitsuhiro; Mikkelsen, Tarjei S.; Nusbaum, Chad; Xie, Xiaohui; Adli, Mazhar; Kasif, Simon; Ptaszek, Leon M.; Koseki, Haruhiko; van Steensel, Bas; Ku, Manching; Koche, Richard Patrick; Mendenhall, Eric M; Presser, Aviva; Chi, Andrew S.; Cowan, Chad A.; Lander, Eric Steven; Bernstein, Bradley E. (Public Library of Science, 2008)
      In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 ...
    • Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA 

      Warren, Luigi; Manos, Philip D.; Ahfeldt, Tim; Loh, Yuin-Han; Li, Hu; Lau, Frank; Ebina, Wataru; Mandal, Pankaj Kumar; Smith, Zachary D; Meissner, Alexander; Daley, George Quentin; Brack, Andrew Stephen; Collins, James J.; Cowan, Chad A.; Schlaeger, Thorsten M.; Rossi, Derrick J (Elsevier BV, 2010)
      Clinical application of induced pluripotent stem cells (iPSCs) is limited by the low efficiency of iPSC derivation and the fact that most protocols modify the genome to effect cellular reprogramming. Moreover, safe and ...
    • Isogenic Human Pluripotent Stem Cell Models of Cardiovascular Disease-Associated Genetic Variation 

      Peters, Derek Tilghman (2016-03-30)
      A complex interplay of genetic and environmental factors underlies the development of common human diseases such as cardiovascular disease. Despite widespread use of existing medications, coronary heart disease (CHD), ...
    • Low Incidence of Off-Target Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human Stem Cell Clones Detected by Whole-Genome Sequencing 

      Veres, Adrian; Gosis, Bridget S.; Ding, Qiurong; Collins, Ryan; Ragavendran, Ashok; Brand, Harrison Andrew; Erdin, Serkan; Cowan, Chad A.; Talkowski, Michael Edward; Musunuru, Kiran (Elsevier BV, 2014)
      Genome editing has attracted wide interest for the generation of cellular models of disease using human pluripotent stem cells and other cell types. CRISPR-Cas systems and TALENs can target desired genomic sites with high ...
    • N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment 

      Soh, Boon-Seng; Buac, Kristina; Xu, Huansheng; Li, Edward; Ng, Shi-Yan; Wu, Hao; Chmielowiec, Jolanta; Jiang, Xin; Bu, Lei; Li, Ronald A; Cowan, Chad; Chien, Kenneth R (Nature Publishing Group, 2014)
      The cardiac progenitor cells (CPCs) in the anterior heart field (AHF) are located in the pharyngeal mesoderm (PM), where they expand, migrate and eventually differentiate into major cell types found in the heart, including ...
    • Optimal Timing of Inner Cell Mass Isolation Increases the Efficiency of Human Embryonic Stem Cell Derivation and Allows Generation of Sibling Cell Lines 

      Chen, Alice E.; Egli, Dieter; Niakan, Kathy; Deng, Jie; Akutsu, Hidenori; Yamaki, Mariko; Cowan, Chad; Fitz-Gerald, Claire; Zhang, Kun; Melton, Douglas A.; Eggan, Kevin; Chen, Alice E.; Egli, Dieter; Niakan, Kathy; Deng, Jie; Akutsu, Hidenori; Yamaki, Mariko; Cowan, Chad A.; Fitz-Gerald, Claire; Zhang, Kun; Melton, Douglas A.; Eggan, Kevin Carl (Elsevier, 2009)
    • Permanent Alteration of PCSK9 With In Vivo CRISPR-Cas9 Genome Editing 

      Ding, Q.; Strong, A.; Patel, K. M.; Ng, S.-L.; Gosis, Bridget Stephanie; Regan, Stephanie N.; Cowan, Chad A.; Rader, D. J.; Musunuru, Kiran (Ovid Technologies (Wolters Kluwer Health), 2014)
      Rationale: Individuals with naturally occurring loss-of-function proprotein convertase subtilisin/kexin type 9 (PCSK9) mutations experience reduced low-density lipoprotein cholesterol levels and protection against ...
    • Programming human pluripotent stem cells into white and brown adipocytes 

      Ahfeldt, Tim; Schinzel, Robert T.; Lee, Youn-Kyoung; Hendrickson, David Gillis; Kaplan, Adam; Lum, David H.; Camahort, Raymond; Xia, Fang; Shay, Jennifer B.; Rhee, Eugene Park; Clish, Clary B.; Deo, Rahul C.; Shen, Tony; Lau, Frank; Cowley, Alicia; Mowrer, Greg; Al-Siddiqi, Heba; Nahrendorf, Matthias; Musunuru, Kiran; Gerszten, Robert Edgardo; Rinn, John L; Cowan, Chad A. (Nature Publishing Group, 2012)
      The utility of human pluripotent stem cells is dependent on efficient differentiation protocols that convert these cells into relevant adult cell types. Here we report the robust and efficient differentiation of human ...
    • A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in postmyocardial infarcted nonhuman primates 

      Blin, Guillaume; Nury, David; Stefanovic, Sonia; Neri, Tui; Guillevic, Oriane; Brinon, Benjamin; Bellamy, Valérie; Rücker-Martin, Catherine; Barbry, Pascal; Bel, Alain; Bruneval, Patrick; Cowan, Chad A.; Pouly, Julia; Mitalipov, Shoukhrat; Gouadon, Elodie; Binder, Patrice; Hagège, Albert; Desnos, Michel; Renaud, Jean-François; Menasché, Philippe; Pucéat, Michel (American Society for Clinical Investigation, 2010)
      Cell therapy holds promise for tissue regeneration, including in individuals with advanced heart failure. However, treatment of heart disease with bone marrow cells and skeletal muscle progenitors has had only marginal ...
    • Rewirable gene regulatory networks in the preimplantation embryonic development of three mammalian species 

      Xie, Dan; Chen, Chieh-Chun; Ptaszek, Leon M.; Xiao, Shu; Cao, Xiaoyi; Fang, Fang; Ng, Huck H.; Lewin, Harry A.; Cowan, Chad A.; Zhong, Sheng (Cold Spring Harbor Laboratory Press, 2010)
      Mammalian preimplantation embryonic development (PED) is thought to be governed by highly conserved processes. While it had been suggested that some plasticity of conserved signaling networks exists among different mammalian ...
    • A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models 

      Ding, Qiurong; Lee, Youn-Kyoung; Schaefer, Esperance Anne Kreek; Peters, Derek Tilghman; Veres, Adrian; Kim, Kevin; Kuperwasser, Nicolas; Motola, Daniel L; Meissner, Torsten B; Hendriks, William; Trevisan, Marta; Gupta, Rajat; Moisan, Annie; Banks, Eric; Friesen, Max; Schinzel, Robert T.; Xia, Fang; Tang, Alexander; Xia, Yulei; Figueroa, Emmanuel; Wann, Amy; Ahfeldt, Tim; Daheron, Laurence Marie; Zhang, Feng; Rubin, Lee; Peng, Lee F; Chung, Raymond Taeyong; Musunuru, Kiran; Cowan, Chad A. (Elsevier BV, 2013)
      Transcription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that are easier to design to cleave at desired sites in a genome than previous types of nucleases. We report here the use of ...