Now showing items 1-8 of 8

    • Activin Signaling Mediates Muscle-to-Adipose Communication in a Mitochondria Dysfunction-Associated Obesity Model 

      Song, Wei; Owusu-Ansah, Edward; Hu, Yanhui; Cheng, Daojun; Ni, Xiaochun; Zirin, Jonathan; Perrimon, Norbert (National Academy of Sciences, 2017-08-08)
      Mitochondrial perturbation-associated dysregulation of one organ has been shown to nonautonomously affect the functions of other organs in both vertebrates and invertebrates. Using Drosophila as a genetic model organism, ...
    • Ecdysone Signaling at Metamorphosis Triggers Apoptosis of Drosophila Abdominal Muscles 

      Zirin, Jonathan; Cheng, Daojun; Dhanyasi, Nagaraju; Cho, Julio; Dura, Jean-Maurice; VijayRaghavan, Krishnaswamy; Perrimon, Norbert (Elsevier BV, 2013-11-15)
      One of the most dramatic examples of programmed cell death occurs during Drosophila metamorphosis, when most of the larval tissues are destroyed in a process termed histolysis. Much of our understanding of this process ...
    • FlyRNAi.org—the database of the Drosophila RNAi screening center and transgenic RNAi project: 2017 update 

      Hu, Yanhui; Comjean, Aram; Roesel, Charles; Vinayagam, Arunachalam; Flockhart, Ian; Zirin, Jonathan; Perkins, Lizabeth; Perrimon, Norbert; Mohr, Stephanie E. (Oxford University Press, 2017)
      The FlyRNAi database of the Drosophila RNAi Screening Center (DRSC) and Transgenic RNAi Project (TRiP) at Harvard Medical School and associated DRSC/TRiP Functional Genomics Resources website (http://fgr.hms.harvard.edu) ...
    • Integrating protein-protein interaction networks with phenotypes reveals signs of interactions 

      Vinayagam, Arunachalam; Zirin, Jonathan; Roesel, Charles; Hu, Yanhui; Yilmazel, Bahar; Samsonova, Anastasia A.; Neumüller, Ralph A.; Mohr, Stephanie E.; Perrimon, Norbert (2013)
      A major objective of systems biology is to organize molecular interactions as networks and to characterize information-flow within networks. We describe a computational framework to integrate protein-protein interaction ...
    • Optimized Strategy for in Vivo Cas9-Activation in Drosophila 

      Ewen-Campen, Benjamin; Yang-Zhou, Donghui; Fernandes, Vitória R.; González, Delfina P.; Liu, Lu-Ping; Tao, Rong; Ren, Xingjie; Sun, Jin; Hu, Yanhui; Zirin, Jonathan; Mohr, Stephanie; Ni, Jian-Quan; Perrimon, Norbert (National Academy of Sciences, 2017-08-29)
      While several large-scale resources are available for in vivo loss-of-function studies in Drosophila, an analogous resource for overexpressing genes from their endogenous loci does not exist. We describe a strategy for ...
    • Primary Cell Cultures from Drosophila Gastrula Embryos 

      Perrimon, Norbert; Zirin, Jonathan; Bai, Jianwu (MyJove Corporation, 2011-02-28)
      Here we describe a method for preparing and culturing primary cells dissociated from Drosophila gastrula embryos. In brief, a large amount of staged embryos from young and healthy flies are collected, sterilized, and then ...
    • Regulators of Autophagosome Formation in Drosophila Muscles 

      Zirin, Jonathan; Nieuwenhuis, Joppe; Samsonova, Anastasia; Tao, Rong; Perrimon, Norbert (Public Library of Science, 2015)
      Given the diversity of autophagy targets and regulation, it is important to characterize autophagy in various cell types and conditions. We used a primary myocyte cell culture system to assay the role of putative autophagy ...
    • Role of Autophagy in Glycogen Breakdown and Its Relevance to Chloroquine Myopathy 

      Zirin, Jonathan; Nieuwenhuis, Joppe; Perrimon, Norbert (Public Library of Science, 2013)
      Several myopathies are associated with defects in autophagic and lysosomal degradation of glycogen, but it remains unclear how glycogen is targeted to the lysosome and what significance this process has for muscle cells. ...