Now showing items 1-3 of 3

    • High-fidelity CRISPR-Cas9 variants with undetectable genome-wide off-targets 

      Kleinstiver, Benjamin P.; Pattanayak, Vikram; Prew, Michelle S.; Tsai, Shengdar Q.; Nguyen, Nhu; Zheng, Zongli; Joung, J. Keith (2015)
      CRISPR-Cas9 nucleases are widely used for genome editing but can induce unwanted off-target mutations. Existing strategies for reducing genome-wide off-targets of the broadly used Streptococcus pyogenes Cas9 (SpCas9) are ...
    • In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites 

      Sander, Jeffry D.; Ramirez, Cherie L.; Linder, Samantha J.; Pattanayak, Vikram; Shoresh, Noam; Ku, Manching; Foden, Jennifer A.; Reyon, Deepak; Bernstein, Bradley E.; Liu, David R.; Joung, J. Keith (Oxford University Press, 2013)
      Gene-editing nucleases enable targeted modification of DNA sequences in living cells, thereby facilitating efficient knockout and precise editing of endogenous loci. Engineered nucleases also have the potential to introduce ...
    • Small Molecule-Triggered Cas9 Protein with Improved Genome-Editing Specificity 

      Davis, Kevin M.; Pattanayak, Vikram; Thompson, David B.; Zuris, John A.; Liu, David R. (2015)
      Directly modulating the activity of genome-editing proteins has the potential to increase their specificity by reducing activity following target locus modification. We developed Cas9 nucleases that are activated by the ...