Browsing by Author "Jedrychowski, Mark"
Now showing items 1-13 of 13
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Adiporedoxin, an upstream regulator of ER oxidative folding and protein secretion in adipocytes
Jedrychowski, Mark P.; Liu, Libin; Laflamme, Collette J.; Karastergiou, Kalypso; Meshulam, Tova; Ding, Shi-Ying; Wu, Yuanyuan; Lee, Mi-Jeong; Gygi, Steven P.; Fried, Susan K.; Pilch, Paul F. (Elsevier, 2015)Objective: Adipocytes are robust protein secretors, most notably of adipokines, hormone-like polypeptides, which act in an endocrine and paracrine fashion to affect numerous physiological processes such as energy balance ... -
eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference
Yi, Tingfang; Arthanari, Haribabu; Akabayov, Barak; Song, Huaidong; Papadopoulos, Evangelos; Qi, Hank H.; Jedrychowski, Mark; Güttler, Thomas; Guo, Cuicui; Luna, Rafael E.; Gygi, Steven P.; Huang, Stephen A.; Wagner, Gerhard (2015)MicroRNA (miRNA) biogenesis and miRNA-guided RNA interference (RNAi) are essential for gene expression in eukaryotes. Here we report that translation initiation factor eIF1A directly interacts with Ago2 and promotes Ago2 ... -
Evaluating Multiplexed Quantitative Phosphopeptide Analysis on a Hybrid Quadrupole Mass Filter/Linear Ion Trap/Orbitrap Mass Spectrometer
Erickson, Brian K.; Jedrychowski, Mark P.; McAlister, Graeme C.; Everley, Robert A.; Kunz, Ryan; Gygi, Steven P. (American Chemical Society, 2014)As a driver for many biological processes, phosphorylation remains an area of intense research interest. Advances in multiplexed quantitation utilizing isobaric tags (e.g., TMT and iTRAQ) have the potential to create a new ... -
Hem-1 Complexes Are Essential for Rac Activation, Actin Polymerization, and Myosin Regulation during Neutrophil Chemotaxis
Weiner, Orion D; Rentel, Maike C; Ott, Alex; Bourne, Henry R; Schliwa, Manfred; Brown, Glenn E.; Jedrychowski, Mark P.; Yaffe, Michael Bruce; Gygi, Steven P.; Cantley, Lewis C.; Kirschner, Marc W. (Public Library of Science, 2006)Migrating cells need to make different actin assemblies at the cell's leading and trailing edges and to maintain physical separation of signals for these assemblies. This asymmetric control of activities represents one ... -
Human DDX3 Functions in Translation and Interacts with the Translation Initiation Factor eIF3
Lee, Chung-Sheng; Dias, Anusha P.; Jedrychowski, Mark P.; Patel, Arvind H.; Hsu, Jeanne L.; Reed, Robin Elizabeth (Oxford University Press, 2008)The conserved RNA helicase DDX3 is of major medical importance due to its involvement in numerous cancers, human hepatitis C virus (HCV) and HIV. Although DDX3 has been reported to have a wide variety of cellular functions, ... -
Identification of a Unique TGF-β Dependent Molecular and Functional Signature in Microglia
Butovsky, Oleg; Jedrychowski, Mark P.; Moore, Craig S.; Cialic, Ron; Lanser, Amanda J.; Gabriely, Galina; Koeglsperger, Thomas; Dake, Ben; Wu, Pauline M.; Doykan, Camille E.; Fanek, Zain; Liu, LiPing; Chen, Zhuoxun; Rothstein, Jeffrey D.; Ransohoff, Richard M.; Gygi, Steven P.; Antel, Jack P.; Weiner, Howard L. (2014)Microglia are myeloid cells of the central nervous system (CNS) that participate both in normal CNS function and disease. We investigated the molecular signature of microglia and identified 239 genes and 8 microRNAs that ... -
Innervation of Thermogenic Adipose Tissue via a Calsyntenin 3β–S100b Axis
Zeng, Xing; Ye, Mengchen; Resch, Jon; Jedrychowski, Mark; Hu, Bo; Lowell, Bradford; Ginty, David; Spiegelman, Bruce (Springer Science and Business Media LLC, 2019-05)The sympathetic nervous system drives brown and beige adipocyte thermogenesis via release of norepinephrine from local axons. However, the molecular basis underlying the higher levels of sympathetic innervation of thermogenic ... -
MultiNotch MS3 Enables Accurate, Sensitive, and Multiplexed Detection of Differential Expression across Cancer Cell Line Proteomes
McAlister, Graeme C.; Nusinow, David P.; Jedrychowski, Mark P.; Wühr, Martin; Huttlin, Edward L.; Erickson, Brian K.; Rad, Ramin; Haas, Wilhelm; Gygi, Steven P. (American Chemical Society, 2014)Multiplexed quantitation via isobaric chemical tags (e.g., tandem mass tags (TMT) and isobaric tags for relative and absolute quantitation (iTRAQ)) has the potential to revolutionize quantitative proteomics. However, until ... -
MURC/Cavin-4 and Cavin Family Members Form Tissue-Specific Caveolar Complexes
Bastiani, Michele; Hill, Michelle M.; Nixon, Susan J.; Lo, Harriet P.; Abankwa, Daniel; Luetterforst, Robert; Fernandez-Rojo, Manuel; Breen, Michael R.; Vinten, Jorgen; Walser, Piers J.; North, Kathryn N.; Hancock, John F.; Pilch, Paul F.; Parton, Robert G.; Liu, Libin; Jedrychowski, Mark P.; Gygi, Steven P. (Rockefeller University Press, 2009)Polymerase I and transcript release factor (PTRF)/Cavin is a cytoplasmic protein whose expression is obligatory for caveola formation. Using biochemistry and fluorescence resonance energy transfer–based approaches, we now ... -
A PGC1-\(\alpha\)-dependent Myokine that Drives Brown-fat-like Development of White Fat and Thermogenesis
Boström, Pontus; Wu, Jun; Jedrychowski, Mark P.; Korde, Anisha; Ye, Li; Lo, James C; Rasbach, Kyle A.; Boström, Elisabeth Almer; Choi, Jang Hyun; Long, Jonathan Zhong; Kajimura, Shingo; Zingaretti, Maria Cristina; Vind, Birgitte F.; Tu, Hua; Cinti, Saverio; Højlund, Kurt; Gygi, Steven P.; Spiegelman, Bruce Michael (Nature Publishing Group, 2012)Exercise benefits a variety of organ systems in mammals, and some of the best-recognized effects of exercise on muscle are mediated by the transcriptional co-activator PPAR-γ co-activator-1 α (PGC1-α). Here we show in mouse ... -
Post-Translational Regulation via Clp Protease Is Critical for Survival of Mycobacterium tuberculosis
Raju, Ravikiran M.; Jedrychowski, Mark P.; Wei, Jun-Rong; Pinkham, Jessica T.; Park, Annie S.; O'Brien, Kathryn; Rehren, German; Schnappinger, Dirk; Gygi, Steven P.; Rubin, Eric J. (Public Library of Science, 2014)Unlike most bacterial species, Mycobacterium tuberculosis depends on the Clp proteolysis system for survival even in in vitro conditions. We hypothesized that Clp is required for the physiologic turnover of mycobacterial ... -
Proteomic and Metabolomic Characterization of a Mammalian Cellular Transition from Quiescence to Proliferation
Lee, Ho-Joon; Jedrychowski, Mark; Vinayagam, Arunachalam; Wu, Ning; Shyh-Chang, Ng; Hu, Yanhui; Min-Wen, Chua; Moore, Jodene; Asara, John; Lyssiotis, Costas A.; Perrimon, Norbert; Gygi, Steven; Cantley, Lewis C.; Kirschner, Marc (Elsevier BV, 2017-07-18)There exist similarities and differences in metabolism and physiology between normal proliferative cells and tumor cells. Once a cell enters the cell cycle, metabolic machinery is engaged to facilitate various processes. ... -
Tetracyclines Promote Survival and Fitness in Mitochondrial Disease Models
Perry, Elizabeth; Bennett, Christopher; Luo, Chi; Balsa Martinez, Eduardo; Jedrychowski, Mark; O'Malley, Katherine; Latorre Muro, Pedro Antonio; Ladley, Richard Porter; Reda, Kamar; Wright, Peter; Gygi, Steven; Myers, Andrew; Puigserver, Pere (Springer Science and Business Media LLC, 2021-01-18)Mitochondrial diseases (MD) are a heterogeneous group of disorders resulting from genetic mutations in nuclear or mitochondrial DNA (mtDNA) genes encoding for mitochondrial proteins 1,2. MD cause pathologies with severe ...