Now showing items 1-9 of 9

    • Co-transcriptional histone methylations 

      Buratowski, Stephen (BioMed Central, 2013)
    • Ctk1 Promotes Dissociation of Basal Transcription Factors from Elongating RNA Polymerase II 

      Ahn, Seong Hoon; Keogh, Michael-Christopher; Buratowski, Stephen (Nature Publishing Group, 2009)
      As RNA polymerase II (RNApII) transitions from initiation to elongation, Mediator and the basal transcription factors TFIID, TFIIA, TFIIH, and TFIIE remain at the promoter as part of a scaffold complex, whereas TFIIB and ...
    • Investigating Mechanisms of DNA Double Strand Break Joining of Switch Regions During IgH Class Switch Recombination 

      Panchakshari, Rohit (2016-05-19)
      During B cell development, RAG endonuclease cleaves immunoglobulin heavy chain (IgH) V, D, and J gene segments and orchestrates their fusion as deletional events that assemble a V(D)J exon in the same transcriptional ...
    • Modulation of mRNA and lncRNA expression dynamics by the Set2–Rpd3S pathway 

      Kim, Ji Hyun; Lee, Bo Bae; Oh, Young Mi; Zhu, Chenchen; Steinmetz, Lars M.; Lee, Yookyeong; Kim, Wan Kyu; Lee, Sung Bae; Buratowski, Stephen; Kim, TaeSoo (Nature Publishing Group, 2016)
      H3K36 methylation by Set2 targets Rpd3S histone deacetylase to transcribed regions of mRNA genes, repressing internal cryptic promoters and slowing elongation. Here we explore the function of this pathway by analysing ...
    • A Molecular Basis for the Coupling of Sister Centromeres 

      Hinshaw, Stephen M. (2016-08-18)
      Chromosome segregation during mitosis requires that two forces act on DNA: one that pulls chromatids away from each other and towards presumptive daughter cells, and another that holds chromatids together and resists the ...
    • Molecular Details and Functional Analysis of RNA Binding by ESCRT-II 

      Emerman, Amy Beth (2015-05-18)
      Many RNAs show distinct localization patterns in cells with enrichment at particular subcellular sites or organelles. RNA localization is a highly conserved process that both spatially and temporally controls gene expression. ...
    • Regulation of microRNA activity by translation initiation factors in melanoma 

      Yanez, Adrienne Gail (2014-06-06)
      microRNAs (miRNAs) are small, noncoding RNAs that may regulate more than half of human genes, yet the molecular mechanism of miRNA-mediated repression remains obscure. Using a cell-free assay of miRNA activity, we show ...
    • Single-Nucleosome Mapping of Histone Modifications in S. cerevisiae 

      Liu, Chih Long; Kaplan, Tommy; Kim, Minkyu; Friedman, Nir; Rando, Oliver J; Buratowski, Stephen; Schreiber, Stuart L. (Public Library of Science, 2005)
      Covalent modification of histone proteins plays a role in virtually every process on eukaryotic DNA, from transcription to DNA repair. Many different residues can be covalently modified, and it has been suggested that these ...
    • Systematic Dissection of Roles for Chromatin Regulators in a Yeast Stress Response 

      Weiner, Assaf; Chen, Hsiuyi V.; Liu, Chih Long; Rahat, Ayelet; Klien, Avital; Soares, Luis; Gudipati, Mohanram; Pfeffner, Jenna; Regev, Aviv; Buratowski, Stephen; Pleiss, Jeffrey A.; Friedman, Nir; Rando, Oliver J. (Public Library of Science, 2012)
      Packaging of eukaryotic genomes into chromatin has wide-ranging effects on gene transcription. Curiously, it is commonly observed that deletion of a global chromatin regulator affects expression of only a limited subset ...