Browsing by Author "Dvorak, Ann"
Now showing items 1-9 of 9
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Human eosinophil leukocytes express protein disulfide isomerase in secretory granules and vesicles: Ultrastructural studies
Dias, F. F.; Amaral, K. B.; Carmo, L. A. S.; Shamri, R.; Dvorak, Ann Marie; Weller, Peter Fahey; Melo, Rossana (Journal of Histochemistry & Cytochemistry (The Histochemical Society), 2014)Protein disulfide isomerase (PDI) has fundamental roles in the oxidative folding of proteins in the endoplasmic reticulum (ER) of eukaryotic cells. The study of this molecule has been attracting considerable attention due ... -
IKK\(\beta\) Regulates Essential Functions of the Vascular Endothelium Through Kinase-Dependent and -Independent Pathways
Ashida, Noboru; SenBanerjee, Sucharita B.; Kodama, Shohta; Foo, Shi Yin; Coggins, Matthew Peter; Spencer, Joel A.; Zamiri, Parisa; Shen, Dongxiao; Li, Ling; Sciuto, Tracey; Dvorak, Ann Marie; Gerszten, Robert Edgardo; Lin, Charles P.; Karin, Michael; Rosenzweig, Anthony (Nature Publishing Group, 2011)Vascular endothelium provides a selective barrier between the blood and tissues, participates in wound healing and angiogenesis, and regulates tissue recruitment of inflammatory cells. Nuclear factor (NF)- \(\kappa\)B ... -
The Internal Architecture of Leukocyte Lipid Body Organelles Captured by Three-Dimensional Electron Microscopy Tomography
Melo, Rossana C. N.; Paganoti, Guillherme F.; Dvorak, Ann Marie; Weller, Peter Fahey (Public Library of Science, 2013)Lipid bodies (LBs), also known as lipid droplets, are complex organelles of all eukaryotic cells linked to a variety of biological functions as well as to the development of human diseases. In cells from the immune system, ... -
Lipid Body–Phagosome Interaction in Macrophages during Infectious Diseases: Host Defense or Pathogen Survival Strategy?
Melo, Rossana C. N.; Dvorak, Ann Marie (Public Library of Science, 2012)Phagocytosis of invading microorganisms by specialized cells such as macrophages and neutrophils is a key component of the innate immune response. These cells capture and engulf pathogens and subsequently destroy them in ... -
Molecular and Morphological Characterization of Piecemeal Degranulation in Human Neutrophil Azurophilic Granules
Mahmudi-Azer, Salahaddin; Weller, Peter Fahey; Dvorak, Ann Marie; Moqbel, Redwan; Paré, Peter D (BioMed Central, 2010) -
Pre-embedding immunogold labeling to optimize protein localization at subcellular compartments and membrane microdomains of leukocytes
Melo, Rossana; Morgan, Ellen; Monahan-Earley, Rita; Dvorak, Ann Marie; Weller, Peter Fahey (Nature Publishing Group, 2014)Precise immunolocalization of proteins within a cell is central to understanding cell processes and functions such as intracellular trafficking and secretion of molecules during immune responses. Here we describe a protocol ... -
Redefining Eosinophil Crystalloid Granules as a Potential New Functional Unit in Extracellular Inflammatory Events
Mahmudi-Azer, Salahaddin; Weller, Peter Fahey; Dvorak, Ann Marie; Moqbel, Redwan; Paré, Peter D (BioMed Central, 2010) -
Release of cellular tension signals self-restorative ventral lamellipodia to heal barrier micro-wounds
Martinelli, Roberta; Kamei, Masataka; Sage, Peter T.; Massol, Ramiro; Varghese, Laya; Sciuto, Tracey; Toporsian, Mourad; Dvorak, Ann M.; Kirchhausen, Tomas; Springer, Timothy A.; Carman, Christopher V. (The Rockefeller University Press, 2013)Basic mechanisms by which cellular barriers sense and respond to integrity disruptions remain poorly understood. Despite its tenuous structure and constitutive exposure to disruptive strains, the vascular endothelium ... -
Vesicle-mediated secretion of human eosinophil granule-derived major basic protein
Melo, Rossana; Spencer, Lisa Ann; Perez, Sandra A C; Neves, Josiane S; Bafford, Staci P; Morgan, Ellen S; Dvorak, Ann Marie; Weller, Peter Fahey (Nature Publishing Group, 2009)Major basic protein (MBP), the predominant cationic protein of human eosinophil specific granules, is stored within crystalloid cores of these granules. Secretion of MBP contributes to the immunopathogenesis of varied ...