Browsing by Author "Hartwig, John"

Now showing items 1-7 of 7

    • Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion 

      Rullo, Jacob; Becker, Henry; Hyduk, Sharon J.; Wong, Janice C.; Digby, Genevieve; Arora, Pamma D.; Cano, Adrianet Puig; Hartwig, John Henry; McCulloch, Christopher A.; Cybulsky, Myron I. (The Rockefeller University Press, 2012)
      Leukocytes arrested on inflamed endothelium via integrins are subjected to force imparted by flowing blood. How leukocytes respond to this force and resist detachment is poorly understood. Live-cell imaging with ...

    • Cytoskeletal Mechanics of Proplatelet Maturation and Platelet Release 

      Thon, Jonathan Noah; Montalvo, Alejandro; Patel-Hett, Sunita; Devine, Matthew T.; Richardson, Jennifer L.; Ehrlicher, Allen Joseph; Larson, Mark K.; Hoffmeister, Karin Maria; Hartwig, John Henry; Italiano, Joseph E. (Rockefeller University Press, 2010)
      Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, ...

    • Documentation and Localization of Force-mediated Filamin A Domain Perturbations in Moving Cells 

      Nakamura, Fumihiko; Song, Mia; Hartwig, John H.; Stossel, Thomas P. (2014)
      Endogenously and externally generated mechanical forces influence diverse cellular activities, a phenomenon defined as mechanotransduction. Deformation of protein domains by application of stress, previously documented to ...

    • Dual roles for hepatic lectin receptors in the clearance of chilled platelets 

      Rumjantseva, Viktoria; Grewal, Prabhjit K.; Wandall, Hans H.; Josefsson, Emma C.; Sørensen, Anne Louise; Larson, Göran; Marth, Jamey D.; Hartwig, John H.; Hoffmeister, Karin M. (2015)
      Chilling rapidly (<4 h) clusters Glycoprotein - (GP)Ib receptors on blood platelets, and ß2-integrins of hepatic macrophages bind ßGlcNAc residues in the clusters leading to rapid clearance of acutely chilled platelets ...

    • Molecular Basis of Filamin A-FilGAP Interaction and Its Impairment in Congenital Disorders Associated with Filamin A Mutations 

      Heikkinen, Outi; Pentikäinen, Olli T.; Kasza, Karen E.; Kupiainen, Olga; Permi, Perttu; Kilpeläinen, Ilkka; Ylänne, Jari; Nakamura, Fumihiko; Osborn, Teresia M.; Weitz, David A.; Hartwig, John Henry; Stossel, Thomas Peter (Public Library of Science, 2009)
      Background: Mutations in filamin A (FLNa), an essential cytoskeletal protein with multiple binding partners, cause developmental anomalies in humans. Methodology/principal findings: We determined the structure of the 23rd ...

    • Paneth cells as a site of origin for intestinal inflammation 

      Adolph, Timon E.; Tomczak, Michal F.; Niederreiter, Lukas; Ko, Hyun-Jeong; Böck, Janne; Martinez-Naves, Eduardo; Glickman, Jonathan N.; Tschurtschenthaler, Markus; Hartwig, John; Hosomi, Shuhei; Flak, Magdalena B.; Cusick, Jennifer L.; Kohno, Kenji; Iwawaki, Takao; Billmann-Born, Susanne; Raine, Tim; Bharti, Richta; Lucius, Ralph; Kweon, Mi-Na; Marciniak, Stefan J.; Choi, Augustine; Hagen, Susan J.; Schreiber, Stefan; Rosenstiel, Philip; Kaser, Arthur; Blumberg, Richard S. (2013)
      Autophagy related 16-like 1 (ATG16L1) as a genetic risk factor has exposed the critical role of autophagy in Crohn’s disease (CD)1. Homozygosity for the highly prevalent ATG16L1 risk allele, or murine hypomorphic (HM) ...

    • Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex 

      Poulter, Natalie S.; Pollitt, Alice Y.; Davies, Amy; Malinova, Dessislava; Nash, Gerard B.; Hannon, Mike J.; Pikramenou, Zoe; Rappoport, Joshua Z.; Hartwig, John H.; Owen, Dylan M.; Thrasher, Adrian J.; Watson, Stephen P.; Thomas, Steven G. (Nature Pub. Group, 2015)
      The actin nodule is a novel F-actin structure present in platelets during early spreading. However, only limited detail is known regarding nodule organization and function. Here we use electron microscopy, SIM and dSTORM ...