Now showing items 1-20 of 43

    • An Adjustable Gas-Mixing Device to Increase Feasibility of In Vitro Culture of Plasmodium falciparum Parasites in the Field 

      Bei, Amy K.; Patel, Saurabh D.; Volkman, Sarah K.; Ahouidi, Ambroise D.; Ndiaye, Daouda; Mboup, Souleymane; Wirth, Dyann F. (Public Library of Science, 2014)
      A challenge to conducting high-impact and reproducible studies of the mechanisms of P. falciparum drug resistance, invasion, virulence, and immunity is the lack of robust and sustainable in vitro culture in the field. While ...
    • Altered drug susceptibility during host adaptation of a Plasmodium falciparum strain in a non-human primate model 

      Obaldía III, Nicanor; Dow, Geoffrey S.; Gerena, Lucia; Kyle, Dennis; Otero, William; Mantel, Pierre-Yves; Baro, Nicholas; Daniels, Rachel; Mukherjee, Angana; Childs, Lauren M.; Buckee, Caroline; Duraisingh, Manoj T.; Volkman, Sarah K.; Wirth, Dyann F.; Marti, Matthias (Nature Publishing Group, 2016)
      Infections with Plasmodium falciparum, the most pathogenic of the Plasmodium species affecting man, have been reduced in part due to artemisinin-based combination therapies. However, artemisinin resistant parasites have ...
    • A broad analysis of resistance development in the malaria parasite 

      Corey, Victoria C.; Lukens, Amanda K.; Istvan, Eva S.; Lee, Marcus C. S.; Franco, Virginia; Magistrado, Pamela; Coburn-Flynn, Olivia; Sakata-Kato, Tomoyo; Fuchs, Olivia; Gnädig, Nina F.; Goldgof, Greg; Linares, Maria; Gomez-Lorenzo, Maria G.; De Cózar, Cristina; Lafuente-Monasterio, Maria Jose; Prats, Sara; Meister, Stephan; Tanaseichuk, Olga; Wree, Melanie; Zhou, Yingyao; Willis, Paul A.; Gamo, Francisco-Javier; Goldberg, Daniel E.; Fidock, David A.; Wirth, Dyann F.; Winzeler, Elizabeth A. (Nature Publishing Group, 2016)
      Microbial resistance to chemotherapy has caused countless deaths where malaria is endemic. Chemotherapy may fail either due to pre-existing resistance or evolution of drug-resistant parasites. Here we use a diverse set of ...
    • Changes in drug sensitivity and anti-malarial drug resistance mutations over time among Plasmodium falciparum parasites in Senegal 

      Van Tyne, Daria; Dieye, Baba; Valim, Clarissa; Daniels, Rachel F; Sène, Papa Diogoye; Lukens, Amanda K; Ndiaye, Mouhamadou; Bei, Amy K; Ndiaye, Yaye Die; Hamilton, Elizabeth J; Ndir, Omar; Mboup, Souleymane; Volkman, Sarah K; Wirth, Dyann F; Ndiaye, Daouda (BioMed Central, 2013)
      Background: Malaria treatment efforts are hindered by the rapid emergence and spread of drug resistant parasites. Simple assays to monitor parasite drug response in direct patient samples (ex vivo) can detect drug resistance ...
    • Confirmation of emergence of mutations associated with atovaquone-proguanil resistance in unexposed Plasmodium falciparum isolates from Africa 

      Happi, Christian Tientcha; Gbotosho, Grace O; Folarin, Onikepe A; Milner, Danny Arnold; Sarr, Ousmane; Sowunmi, Akintunde; Kyle, Dennis E; Milhous, Wilbur K; Wirth, Dyann Fergus; Oduola, Ayoade MJ (BioMed Central, 2006)
      Background: In vitro and in vivo resistance of Plasmodium falciparum to atovaquone or atovaquone-proguanil hydrochloride combination has been associated to two point mutations in the parasite cytochrome b (cytb) gene ...
    • Development of a Single Nucleotide Polymorphism Barcode to Genotype Plasmodium vivax Infections 

      Baniecki, Mary Lynn; Faust, Aubrey L.; Schaffner, Stephen F.; Park, Daniel J.; Galinsky, Kevin; Daniels, Rachel F.; Hamilton, Elizabeth; Ferreira, Marcelo U.; Karunaweera, Nadira D.; Serre, David; Zimmerman, Peter A.; Sá, Juliana M.; Wellems, Thomas E.; Musset, Lise; Legrand, Eric; Melnikov, Alexandre; Neafsey, Daniel E.; Volkman, Sarah K.; Wirth, Dyann F.; Sabeti, Pardis C. (Public Library of Science, 2015)
      Plasmodium vivax, one of the five species of Plasmodium parasites that cause human malaria, is responsible for 25–40% of malaria cases worldwide. Malaria global elimination efforts will benefit from accurate and effective ...
    • Dietary restriction protects against experimental cerebral malaria via leptin modulation and T cell mTORC1 suppression 

      Mejia, Pedro; Treviño-Villarreal, J. Humberto; Hine, Christopher; Harputlugil, Eylul; Lang, Samantha; Calay, Ediz; Rogers, Rick; Wirth, Dyann; Duraisingh, Manoj T.; Mitchell, James R. (2014)
      Host nutrition can affect the outcome of parasitic diseases through metabolic effects on host immunity and/or the parasite. Here we show that modulation of mouse immunometabolism through brief restriction of food intake ...
    • Diversity-Oriented Synthesis Probe Targets Plasmodium falciparum Cytochrome b Ubiquinone Reduction Site and Synergizes With Oxidation Site Inhibitors 

      Lukens, Amanda K.; Heidebrecht, Richard W.; Mulrooney, Carol; Beaudoin, Jennifer A.; Comer, Eamon; Duvall, Jeremy R.; Fitzgerald, Mark E.; Masi, Daniela; Galinsky, Kevin; Scherer, Christina A.; Palmer, Michelle; Munoz, Benito; Foley, Michael; Schreiber, Stuart L.; Wiegand, Roger C.; Wirth, Dyann F. (Oxford University Press, 2014)
      Background. The emergence and spread of drug resistance to current antimalarial therapies remains a pressing concern, escalating the need for compounds that demonstrate novel modes of action. Diversity-Oriented Synthesis ...
    • Diversity-Oriented Synthesis Yields a Novel Lead for the Treatment of Malaria 

      Mulrooney, Carol; Austin, Christopher P.; Beaudoin, Jennifer A.; Cheng, Ken Chih-Chien; Comer, Eamon; Dandapani, Sivaraman; Dick, Justin; Duvall, Jeremy R.; Ekland, Eric H.; Fidock, David A.; Guha, Rajarshi; Hinkson, Paul; Kramer, Martin; Masi, Daniela; Marcaurelle, Lisa A.; Su, Xin-Zhuan; Weïwer, Michel; Xia, Menghang; Yuan, Jing; Zhao, Jinghua; Palmer, Michelle; Munoz, Benito; Heidebrecht, Richard W; Barker, Robert Howard; Fitzgerald, Mark E.; Foley, Michael Raymond; Lukens, Amanda Kathleen; Thomas, Craig J.; Wiegand, Roger C.; Wirth, Dyann Fergus; Schreiber, Stuart L. (AmericanChemical Society, 2011)
      Here, we describe the discovery of a novel antimalarial agent using phenotypic screening of Plasmodium falciparum asexual blood-stage parasites. Screening a novel compound collection created using diversity-oriented synthesis ...
    • Diversity-Oriented Synthesis-Facilitated Medicinal Chemistry: Toward the Development of Novel Antimalarial Agents 

      Comer, Eamon; Beaudoin, Jennifer A.; Kato, Nobutaka; Fitzgerald, Mark E.; Heidebrecht, Richard W.; Lee, Maurice duPont; Masi, Daniela; Mercier, Marion; Mulrooney, Carol; Muncipinto, Giovanni; Rowley, Ann; Crespo-Llado, Keila; Serrano, Adelfa E.; Lukens, Amanda K.; Wiegand, Roger C.; Wirth, Dyann F.; Palmer, Michelle A.; Foley, Michael A.; Munoz, Benito; Scherer, Christina A.; Duvall, Jeremy R.; Schreiber, Stuart L. (American Chemical Society, 2014)
      Here, we describe medicinal chemistry that was accelerated by a diversity-oriented synthesis (DOS) pathway, and in vivo studies of our previously reported macrocyclic antimalarial agent that derived from the synthetic ...
    • Enrichment of Reticulocytes from Whole Blood using Aqueous Multiphase Systems of Polymers 

      Kumar, Ashok Ashwin; Lim, Caeul; Moreno, Yovany; Mace, Charles R.; Syed, Abeer; Van Tyne, Daria Natalie; Wirth, Dyann F.; Duraisingh, Manoj T.; Whitesides, George McClelland (Wiley-Blackwell, 2014)
      This paper demonstrates the enrichment of reticulocytes by centrifuging whole blood through aqueous multiphase systems (AMPSs)—immiscible phases of solutions of polymers that form step-gradients in density. The interfaces ...
    • Evidence for spleen dysfunction in malaria-HIV co-infection in a subset of pediatric patients 

      Joice, Regina; Frantzreb, Charles; Pradham, Alana; Seydel, Karl B.; Kamiza, Steve; Wirth, Dyann F.; Duraisingh, Manoj T.; Molyneux, Malcolm E; Taylor, Terrie E.; Marti, Matthias; Milner, Danny A. (2015)
      The spleen has an important role in the clearance of malaria parasites, and the role of HIV co-infection on this process is yet to be described. Using a combination of histological and molecular methods, we systematically ...
    • A General SNP-Based Molecular Barcode for Plasmodium Falciparum Identification and Tracking 

      Mahesh, Nira; Rosen, David; Daniels, Rachel; Volkman, Sarah K.; Milner, Danny Arnold; Neafsey, Daniel Edward; Park, Daniel John; Angelino, Elaine; Sabeti, Pardis Christine; Wirth, Dyann Fergus; Wiegand, Roger C. (BioMed Central, 2008)
      Background: Single nucleotide polymorphism (SNP) genotyping provides the means to develop a practical, rapid, inexpensive assay that will uniquely identify any Plasmodium falciparum parasite using a small amount of DNA. ...
    • Genetic relatedness analysis reveals the cotransmission of genetically related Plasmodium falciparum parasites in Thiès, Senegal 

      Wong, Wesley; Griggs, Allison D.; Daniels, Rachel F.; Schaffner, Stephen F.; Ndiaye, Daouda; Bei, Amy K.; Deme, Awa B.; MacInnis, Bronwyn; Volkman, Sarah K.; Hartl, Daniel L.; Neafsey, Daniel E.; Wirth, Dyann F. (BioMed Central, 2017)
      Background: As public health interventions drive parasite populations to elimination, genetic epidemiology models that incorporate population genomics can be powerful tools for evaluating the effectiveness of continued ...
    • Genetic Surveillance Detects Both Clonal and Epidemic Transmission of Malaria following Enhanced Intervention in Senegal 

      Daniels, Rachel Fath; Chang, Hsiao-Han; Séne, Papa Diogoye; Park, Danny C.; Neafsey, Daniel Edward; Schaffner, Stephen; Hamilton, Elizabeth Julia; Lukens, Amanda Kathleen; Van Tyne, Daria Natalie; Mboup, Souleymane; Sabeti, Pardis Christine; Ndiaye, Daouda; Wirth, Dyann Fergus; Hartl, Daniel L.; Cooke, Sarah Volkman (Public Library of Science, 2013)
      Using parasite genotyping tools, we screened patients with mild uncomplicated malaria seeking treatment at a clinic in Thiès, Senegal, from 2006 to 2011. We identified a growing frequency of infections caused by genetically ...
    • Genome-Wide SNP Genotyping Highlights the Role of Natural Selection in Plasmodium Falciparum Population Divergence 

      Wirth, Dyann F.; Sabeti, Pardis C.; Lander, Eric S.; Birren, Bruce W.; Hartl, Daniel; Wiegand, Roger; Chitnis, Chetan E.; Dash, Aditya P.; do Lago Moraes, Sandra; Ferreira, Marcelo U.; Mboup, Soulyemane; Ndir, Omar; Ndiaye, Daouda; Ousmane, Sarr; Stange-Thomann, Nicole; Gates, Casey; Tyndall, Erin; Cortese, Joseph F.; Houde, Nathan; Daniels, Rachel; Rosen, David; Lukens, Amanda; Milner, Danny; Montgomery, Philip; Park, Daniel; Volkman, Sarah K.; Schaffner, Stephen F.; Neafsey, Daniel (BioMed Central, 2008)
      Background: The malaria parasite Plasmodium falciparum exhibits abundant genetic diversity, and this diversity is key to its success as a pathogen. Previous efforts to study genetic diversity in P. falciparum have begun ...
    • A genomic and evolutionary approach reveals non-genetic drug resistance in malaria 

      Herman, Jonathan D; Rice, Daniel P; Ribacke, Ulf; Silterra, Jacob; Deik, Amy A; Moss, Eli L; Broadbent, Kate M; Neafsey, Daniel E; Desai, Michael M; Clish, Clary B; Mazitschek, Ralph; Wirth, Dyann F (BioMed Central, 2014)
      Background: Drug resistance remains a major public health challenge for malaria treatment and eradication. Individual loci associated with drug resistance to many antimalarials have been identified, but their epistasis ...
    • Genomic Tools Reveal Changing Plasmodium falciparum Populations 

      Daniels, Rachel Fath (2013-09-25)
      A new era of malaria eradication programs relies on increased knowledge of the parasite through sequencing of the Plasmodium genome. Programs call for re-orientation at specific epidemiological markers as regions move from ...
    • A Global Transcriptional Analysis of Plasmodium Falciparum Malaria Reveals A Novel Family of Telomere-Associated lncRNAs 

      Broadbent, Kate Mariel; Park, Daniel John; Wolf, Ashley Robin; Van Tyne, Daria Natalie; Sims, Jennifer Sung; Ribacke, Ulf Erik; Cooke, Sarah Volkman; Duraisingh, Manoj T.; Wirth, Dyann Fergus; Sabeti, Pardis Christine; Rinn, John L (BioMed Central, 2011)
      Background: Mounting evidence suggests a major role for epigenetic feedback in Plasmodium falciparum transcriptional regulation. Long non-coding RNAs (lncRNAs) have recently emerged as a new paradigm in epigenetic ...
    • Halofuginone: A Story of How Target Identification of an Ancient Chinese Medicine and Multi-Step Evolution Informs Malaria Drug Discovery 

      Herman, Jonathan David (2014-06-06)
      Malaria is a treatable communicable disease yet remains a common cause of death and disease especially among pregnant women and children. Most of malaria's worldwide burden disproportionately lies in Southeast Asia and ...