Browsing by Author "Sabatini, Bernardo"
Now showing items 1-20 of 24
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All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins
Hochbaum, Daniel R.; Zhao, Yongxin; Farhi, Samouil L.; Klapoetke, Nathan; Werley, Christopher A.; Kapoor, Vikrant; Zou, Peng; Kralj, Joel M.; Maclaurin, Dougal; Smedemark-Margulies, Niklas; Saulnier, Jessica L.; Boulting, Gabriella L.; Straub, Christoph; Cho, Yong Ku; Melkonian, Michael; Wong, Gane Ka-Shu; Harrison, D. Jed; Murthy, Venkatesh N.; Sabatini, Bernardo; Boyden, Edward S.; Campbell, Robert E.; Cohen, Adam E. (2014)All-optical electrophysiology—spatially resolved simultaneous optical perturbation and measurement of membrane voltage—would open new vistas in neuroscience research. We evolved two archaerhodopsin-based voltage indicators, ... -
Basal Ganglia Modulation of Cortical Firing Rates in a Behaving Animal
Oldenburg, Ian Anton (2014-10-22)Motor cortex, basal ganglia (BG), and thalamus are anatomically arranged in a recurrent loop whose activity is hypothesized to be involved in the selection of motor actions. Direct (dSPN) and indirect (iSPN) striatal ... -
Cellular Metabolism Modulates Ion Channels That Regulate Neuronal Excitability
Lutas, Andrew (2015-05-01)Epilepsy is a common neurological disorder, affecting around 1% of the world’s population. For many, drugs are available that can prevent their seizures. However, for close to one third of those who suffer from epilepsy, ... -
Cholinergic Interneurons Mediate Fast VGluT3-Dependent Glutamatergic Transmission in the Striatum
Higley, Michael J.; Gittis, Aryn H.; Oldenburg, Ian Anton; Balthasar, Nina; Seal, Rebecca P.; Edwards, Robert H.; Lowell, Bradford Barr; Kreitzer, Anatol C.; Sabatini, Bernardo Luis (Public Library of Science, 2011)The neurotransmitter glutamate is released by excitatory projection neurons throughout the brain. However, non-glutamatergic cells, including cholinergic and monoaminergic neurons, express markers that suggest that they ... -
Circuit interactions between the cortex and basal ganglia
Saunders, Arpiar Bruce (2014-10-23)All animals must adapt their behaviors by experience to survive. In mammals, this adaptive process is thought occur through a synaptic loop involving the cortex, basal ganglia (BG) and thalamus. Here we use transgenic mice ... -
Corelease of acetylcholine and GABA from cholinergic forebrain neurons
Saunders, Arpiar; Granger, Adam J; Sabatini, Bernardo L (eLife Sciences Publications, Ltd, 2015)Neurotransmitter corelease is emerging as a common theme of central neuromodulatory systems. Though corelease of glutamate or GABA with acetylcholine has been reported within the cholinergic system, the full extent is ... -
Cortical synaptogenesis and excitatory synapse number are determined via a Neuroligin-1-dependent intercellular competition
Kwon, Hyung-Bae; Kozorovitskiy, Yevgenia; Oh, Won-Jong; Peixoto, Rui T.; Akhtar, Nazia; Saulnier, Jessica L.; Gu, Chenghua; Sabatini, Bernardo L. (2012)Members of the neuroligin (NL) family of cell-adhesion proteins are found at excitatory and inhibitory synapses and are mutated in some familial forms of autism spectrum disorders. Although they display synaptogenic ... -
CRISPR/Cas9-Mediated Gene Knock-Down in Post-Mitotic Neurons
Straub, Christoph; Granger, Adam J.; Saulnier, Jessica L.; Sabatini, Bernardo L. (Public Library of Science, 2014)The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for genome editing in eukaryotic cells. This technique allows for sequence-specific induction of double-strand breaks in genomic DNA of individual ... -
Development and Application of Two-Photon Excitation Stimulated Emission Depletion Microscopy for Superresolution Fluorescence Imaging in Thick Tissue
Takasaki, Kevin Takao (2013-09-18)Two-photon laser scanning microscopy (2PLSM) allows fluorescence imaging in thick biological samples where absorption and scattering typically degrade resolution and signal collection of 1-photon imaging approaches. The ... -
A direct GABAergic output from the basal ganglia to frontal cortex
Saunders, Arpiar; Oldenburg, Ian A.; Berezovskii, Vladimir K.; Johnson, Caroline A.; Kingery, Nathan D.; Elliott, Hunter L.; Xie, Tiao; Gerfen, Charles R.; Sabatini, Bernardo L. (2014)The basal ganglia (BG) are phylogenetically conserved subcortical nuclei necessary for coordinated motor action and reward learning1. Current models postulate that the BG modulate cerebral cortex indirectly via an inhibitory ... -
A Direct Projection from Mouse Primary Visual Cortex to Dorsomedial Striatum
Khibnik, Lena A.; Tritsch, Nicolas X.; Sabatini, Bernardo L. (Public Library of Science, 2014)The mammalian striatum receives inputs from many cortical areas, but the existence of a direct axonal projection from the primary visual cortex (V1) is controversial. In this study we use anterograde and retrograde tracing ... -
Dopaminergic neurons inhibit striatal output via non-canonical release of GABA
Tritsch, Nicolas X.; Ding, Jun B.; Sabatini, Bernardo L. (2012)The substantia nigra pars compacta (SNc) and ventral tegmental area (VTA) contain the two largest populations of dopamine (DA)-releasing neurons in the mammalian brain. These neurons extend elaborate projections in striatum, ... -
Early hyperactivity and precocious maturation of corticostriatal circuits in Shank3B−/− mice
Peixoto, Rui T.; Wang, Wengang; Croney, Donyell M.; Kozorovitskiy, Yevgenia; Sabatini, Bernardo L. (2016)Some autistic individuals exhibit abnormal development of the caudate nucleus and associative cortical areas, suggesting potential dysfunction of cortico-basal ganglia (BG) circuits. Using optogenetic and electrophysiological ... -
Globus Pallidus Externus Neurons Expressing parvalbumin Interconnect the Subthalamic Nucleus and Striatal Interneurons
Saunders, Arpiar; Huang, Kee Wui; Sabatini, Bernardo Luis (Public Library of Science, 2016)The globus pallidus externus (GP) is a nucleus of the basal ganglia (BG), containing GABAergic projection neurons that arborize widely throughout the BG, thalamus and cortex. Ongoing work seeks to map axonal projection ... -
High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons
Nieland, Thomas J. F.; Logan, David J.; Saulnier, Jessica; Lam, Daniel; Johnson, Caroline; Root, David E.; Carpenter, Anne E.; Sabatini, Bernardo L. (Public Library of Science, 2014)The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation ... -
Midbrain dopamine neurons sustain inhibitory transmission using plasma membrane uptake of GABA, not synthesis
Tritsch, Nicolas X; Oh, Won-Jong; Gu, Chenghua; Sabatini, Bernardo L (eLife Sciences Publications, Ltd, 2014)Synaptic transmission between midbrain dopamine neurons and target neurons in the striatum is essential for the selection and reinforcement of movements. Recent evidence indicates that nigrostriatal dopamine neurons inhibit ... -
Neuromodulation of excitatory synaptogenesis in striatal development
Kozorovitskiy, Yevgenia; Peixoto, Rui; Wang, Wengang; Saunders, Arpiar; Sabatini, Bernardo L (eLife Sciences Publications, Ltd, 2015)Dopamine is released in the striatum during development and impacts the activity of Protein Kinase A (PKA) in striatal spiny projection neurons (SPNs). We examined whether dopaminergic neuromodulation regulates activity-dependent ... -
Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons
Saunders, Arpiar Bruce; Johnson, Caroline; Sabatini, Bernardo Luis (Frontiers Media S.A., 2012)Understanding the organization of the nervous system requires methods for dissecting the contributions of each component cell type to circuit function. One widely used approach combines genetic targeting of Cre recombinase ... -
A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging
Chen, Yao; Saulnier, Jessica L.; Yellen, Gary; Sabatini, Bernardo L. (Frontiers Media S.A., 2014)Neuromodulators have profound effects on behavior, but the dynamics of their intracellular effectors has remained unclear. Most neuromodulators exert their function via G-protein-coupled receptors (GPCRs). One major challenge ... -
Recurrent network activity drives striatal synaptogenesis
Kozorovitskiy, Yevgenia; Saunders, Arpiar; Johnson, Caroline A.; Lowell, Bradford B.; Sabatini, Bernardo L. (2012)Neural activity during development critically shapes postnatal wiring of the mammalian brain. This is best illustrated by the sensory systems, in which the patterned feed-forward excitation provided by sensory organs and ...