Now showing items 1-3 of 3

    • A Genome-Scale shRNA Resource for Transgenic RNAi in Drosophila 

      Ni, Jian-Quan; Zhou, Rui; Czech, Benjamin; Liu, Lu-Ping; Holderbaum, Laura; Yang-Zhou, Donghui; Shim, Hye-Seok; Tao, Rong; Handler, Dominik; Karpowicz, Phillip; Binari, Richard; Booker, Matthew; Brennecke, Julius; Perkins, Lizabeth A.; Hannon, Gregory J.; Perrimon, Norbert (Springer Science and Business Media LLC, 2011-05)
      Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important ...
    • Optimized Strategy for in Vivo Cas9-Activation in Drosophila 

      Ewen-Campen, Benjamin; Yang-Zhou, Donghui; Fernandes, Vitória R.; González, Delfina P.; Liu, Lu-Ping; Tao, Rong; Ren, Xingjie; Sun, Jin; Hu, Yanhui; Zirin, Jonathan; Mohr, Stephanie; Ni, Jian-Quan; Perrimon, Norbert (National Academy of Sciences, 2017-08-29)
      While several large-scale resources are available for in vivo loss-of-function studies in Drosophila, an analogous resource for overexpressing genes from their endogenous loci does not exist. We describe a strategy for ...
    • Regulators of Autophagosome Formation in Drosophila Muscles 

      Zirin, Jonathan; Nieuwenhuis, Joppe; Samsonova, Anastasia; Tao, Rong; Perrimon, Norbert (Public Library of Science, 2015)
      Given the diversity of autophagy targets and regulation, it is important to characterize autophagy in various cell types and conditions. We used a primary myocyte cell culture system to assay the role of putative autophagy ...