Browsing by Author "Brown, Dennis"

Now showing items 1-13 of 13

    • The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer 

      Cotter, Kristina; Liberman, Rachel; Sun-Wada, GeHong; Wada, Yoh; Sgroi, Dennis; Naber, Stephen; Brown, Dennis; Breton, Sylvie; Forgac, Michael (Impact Journals LLC, 2016)
      The vacuolar (H+)-ATPases (V-ATPases) are a family of ATP-driven proton pumps that acidify intracellular compartments and transport protons across the plasma membrane. Previous work has demonstrated that plasma membrane ...

    • AQP2 is Necessary for Vasopressin- and Forskolin-Mediated Filamentous Actin Depolymerization in Renal Epithelial Cells 

      Yui, Naofumi; Lu, Hua A Jenny; Bouley, Richard; Brown, Dennis (The Company of Biologists, 2011)
      Remodeling of the actin cytoskeleton is required for vasopressin (VP)‐induced aquaporin 2 (AQP2) trafficking. Here, we asked whether VP and forskolin (FK)‐mediated F‐actin depolymerization depends on AQP2 expression. Using ...

    • Differential, Phosphorylation Dependent Trafficking of AQP2 in LLC-PK1 Cells 

      Rice, William; Zhang, Yan; Chen, Ying; Matsuzaki, Toshiyuki; Brown, Dennis; Lu, Hua A Jenny (Public Library of Science, 2012)
      The kidney maintains water homeostasis by modulating aquaporin 2 (AQP2) on the plasma membrane of collecting duct principal cells in response to vasopressin (VP). VP mediated phosphorylation of AQP2 at serine 256 is critical ...

    • An Extended Nomenclature for Mammalian V-ATPase Subunit Genes and Splice Variants 

      Miranda, Kevin C.; Karet, Fiona E.; Ruttenberg, Alan; Brown, Dennis (Public Library of Science, 2010)
      The vacuolar-type H+-ATPase (V-ATPase) is a multisubunit proton pump that is involved in both intra- and extracellular acidification processes throughout the body. Multiple homologs and splice variants of V-ATPase subunits ...

    • High Resolution Helium Ion Scanning Microscopy of the Rat Kidney 

      Rice, William; Van Hoek, Alfred N.; Păunescu, Teodor G.; Huynh, Chuong; Goetze, Bernhard; Singh, Bipin; Scipioni, Larry; Stern, Lewis A.; Brown, Dennis (Public Library of Science, 2013)
      Helium ion scanning microscopy is a novel imaging technology with the potential to provide sub-nanometer resolution images of uncoated biological tissues. So far, however, it has been used mainly in materials science ...

    • Loss of the V-ATPase B1 Subunit Isoform Expressed in Non-Neuronal Cells of the Mouse Olfactory Epithelium Impairs Olfactory Function 

      Paunescu, Teodor Gabriel; Rodriguez, Steven; Benz, Eric; McKee, Mary; Tyszkowski, Robert; Albers, Mark W.; Brown, Dennis (Public Library of Science, 2012)
      The vacuolar proton-pumping ATPase (V-ATPase) is the main mediator of intracellular organelle acidification and also regulates transmembrane proton (H+) secretion, which is necessary for an array of physiological functions ...

    • Mapping the H+ (V)-ATPase interactome: identification of proteins involved in trafficking, folding, assembly and phosphorylation 

      Merkulova, Maria; Păunescu, Teodor G.; Azroyan, Anie; Marshansky, Vladimir; Breton, Sylvie; Brown, Dennis (Nature Publishing Group, 2015)
      V-ATPases (H+ ATPases) are multisubunit, ATP-dependent proton pumps that regulate pH homeostasis in virtually all eukaryotes. They are involved in key cell biological processes including vesicle trafficking, endosomal pH ...

    • Massively Parallel Sequencing of Human Urinary Exosome/Microvesicle RNA Reveals a Predominance of Non-Coding RNA 

      Miranda, Kevin C.; Bond, Daniel T.; Levin, Joshua Z.; Adiconis, Xian; Sivachenko, Andrey; Russ, Carsten; Brown, Dennis; Nusbaum, Chad; Russo, Leileata M. (Public Library of Science, 2014)
      Intact RNA from exosomes/microvesicles (collectively referred to as microvesicles) has sparked much interest as potential biomarkers for the non-invasive analysis of disease. Here we use the Illumina Genome Analyzer to ...

    • A Mouse Model for Distal Renal Tubular Acidosis Reveals a Previously Unrecognized Role of the V-ATPase a4 Subunit in the Proximal Tubule 

      Hennings, J Christopher; Picard, Nicolas; Huebner, Antje K; Stauber, Tobias; Maier, Hannes; Brown, Dennis; Jentsch, Thomas J; Vargas-Poussou, Rosa; Eladari, Dominique; Hübner, Christian A (Wiley, 2012)
      The V-ATPase is a multisubunit complex that transports protons across membranes. Mutations of its B1 or a4 subunit are associated with distal renal tubular acidosis and deafness. In the kidney, the a4 subunit is expressed ...

    • Myocardial Infarction Activates CCR2+ Hematopoietic Stem and Progenitor Cells 

      Dutta, Partha; Sager, Hendrik B; Stengel, Kristy R.; Naxerova, Kamila; Courties, Gabriel P; Saez, Borja; Silberstein, Lev; Heidt, Timo; Sebas, Matthew; Sun, Yuan; Wojtkiewicz, Gregory; Feruglio, Paolo Fumene; King, Kevin Robert; Baker, Joshua N.; van der Laan, Anja M.; Borodovsky, Anna; Fitzgerald, Kevin; Hulsmans, Maarten; Hoyer, Friedrich Felix; Iwamoto, Yoshiko; Vinegoni, Claudio; Brown, Dennis; Di Carli, Marcelo; Libby, Peter; Hiebert, Scott W.; Scadden, David Thomas; Swirski, Filip K.; Weissleder, Ralph; Nahrendorf, Matthias (Elsevier BV, 2015)
      Following myocardial infarction (MI), myeloid cells derived from the hematopoietic system drive a sharp increase in systemic leukocyte levels that correlates closely with mortality. The origin of these myeloid cells, and ...

    • Renal Intercalated Cells Sense and Mediate Inflammation via the P2Y14 Receptor 

      Azroyan, Anie; Cortez-Retamozo, Virna; Bouley, Richard; Liberman, Rachel; Ruan, Ye Chun; Kiselev, Evgeny; Jacobson, Kenneth A.; Pittet, Mikael J.; Brown, Dennis; Breton, Sylvie (Public Library of Science, 2015)
      Uncontrolled inflammation is one of the leading causes of kidney failure. Pro-inflammatory responses can occur in the absence of infection, a process called sterile inflammation. Here we show that the purinergic receptor ...

    • Tyrosine kinase-mediated axial motility of basal cells revealed by intravital imaging 

      Roy, Jeremy; Kim, Bongki; Hill, Eric; Visconti, Pablo; Krapf, Dario; Vinegoni, Claudio; Weissleder, Ralph; Brown, Dennis; Breton, Sylvie (Nature Publishing Group, 2016)
      Epithelial cells are generally considered to be static relative to their neighbours. Basal cells in pseudostratified epithelia display a single long cytoplasmic process that can cross the tight junction barrier to reach ...

    • The V‐ATPase is expressed in the choroid plexus and mediates cAMP‐induced intracellular pH alterations 

      Christensen, Henriette L.; Păunescu, Teodor G.; Matchkov, Vladimir; Barbuskaite, Dagne; Brown, Dennis; Damkier, Helle H.; Praetorius, Jeppe (John Wiley and Sons Inc., 2017)
      Abstract The cerebrospinal fluid (CSF) pH influences brain interstitial pH and, therefore, brain function. We hypothesized that the choroid plexus epithelium (CPE) expresses the vacuolar H+‐ATPase (V‐ATPase) as an acid ...