Now showing items 1-20 of 29

    • Analysis of Thyroid Response Element Activity During Retinal Development 

      Billings, Nathan; Emerson, Mark Monroe; Cepko, Connie (Public Library of Science, 2010)
      Thyroid hormone (TH) signaling components are expressed during retinal development in dynamic spatial and temporal patterns. To probe the competence of retinal cells to mount a transcriptional response to TH, reporters ...
    • Cell Type-Specific Manipulation with GFP-Dependent Cre Recombinase 

      Tang, Jonathan C Y; Rudolph, Stephanie; Dhande, Onkar S; Abraira, Victoria E; Choi, Seungwon; Lapan, Sylvain; Drew, Iain R; Drokhlyansky, Eugene; Huberman, Andrew D; Regehr, Wade G; Cepko, Constance L (2016)
      Summary There are many transgenic GFP reporter lines that allow visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables ...
    • The Cis-regulatory Logic of the Mammalian Photoreceptor Transcriptional Network 

      Hsiau, Timothy H.-C.; Diaconu, Claudiu; Myers, Connie A.; Lee, Jongwoo; Cepko, Constance L.; Corbo, Joseph C. (Public Library of Science, 2007)
      The photoreceptor cells of the retina are subject to a greater number of genetic diseases than any other cell type in the human body. The majority of more than 120 cloned human blindness genes are highly expressed in ...
    • Clustering Analysis of SAGE Data using a Poisson Approach 

      Cai, Li; Huang, Haiyan; Blackshaw, Seth; Liu, Jun; Cepko, Connie; Wong, Wing H. (BioMed Central, 2004)
      Serial analysis of gene expression (SAGE) data have been poorly exploited by clustering analysis owing to the lack of appropriate statistical methods that consider their specific properties. We modeled SAGE data by Poisson ...
    • Co-opting Intracellular Proteins for Cell-Specific Gene Manipulation 

      Tang, Chung Yiu Jonathan (2015-01-22)
      Studies of complex multicellular organisms would benefit from the ability to selectively manipulate the activities of any cell type of interest. Our ability to achieve this is currently limited by technology and available ...
    • Comparison of Hybridization-based and Sequencing-based Gene Expression Technologies on Biological Replicates 

      Liu, Fang; Jenssen, Tor-Kristian; Trimarchi, Jeff; Punzo, Claudio; Cepko, Connie; Ohno-Machado, Lucila; Hovig, Eivind; Patrick Kuo, Winston (BioMed Central, 2007)
      Background: High-throughput systems for gene expression profiling have been developed and have matured rapidly through the past decade. Broadly, these can be divided into two categories: hybridization-based and sequencing-based ...
    • Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies 

      Tang, Jonathan CY; Drokhlyansky, Eugene; Etemad, Behzad; Rudolph, Stephanie; Guo, Binggege; Wang, Sui; Ellis, Emily G; Li, Jonathan Z; Cepko, Constance L (eLife Sciences Publications, Ltd, 2016)
      The ability to detect and/or manipulate specific cell populations based upon the presence of intracellular protein epitopes would enable many types of studies and applications. Protein binders such as nanobodies (Nbs) can ...
    • Functional Analysis of Notch Signaling during Vertebrate Retinal Development 

      Mizeracka, Karolina (2013-02-25)
      The process of cell fate determination, which establishes the vastly diverse set of neural cell types found in the central nervous system, remains poorly understood. During retinal development, multipotent retinal progenitor ...
    • Gene Expression Changes within Müller Glial Cells in Retinitis Pigmentosa 

      Roesch, Karin; Stadler, Michael B.; Cepko, Connie (Molecular Vision, 2012)
      Purpose: Retinitis pigmentosa (RP) is a progressive retinal degeneration in which the retina loses nearly all of its photoreceptor cells and undergoes major structural changes. Little is known regarding the role the resident ...
    • Haploinsufficient Bmp4 Ocular Phenotypes Include Anterior Segment Dysgenesis with Elevated Intraocular Pressure 

      Chang, Bo; Peters, Maureen; Zabaleta, Adriana; Nusinowitz, Steven; Smith, Richard S.; Savinova, Olga V.; Hawes, Norman L.; Martin, Janice E.; Davisson, Muriel L.; Cepko, Connie; Hogan, Brigid L. M.; John, Simon W. M. (BioMed Central, 2001)
      Background: Glaucoma is a blinding disease usually associated with high intraocular pressure (IOP). In some families, abnormal anterior segment development contributes to glaucoma. The genes causing anterior segment ...
    • A Hybrid Photoreceptor Expressing Both Rod and Cone Genes in a Mouse Model of Enhanced S-Cone Syndrome 

      Corbo, Joseph C; Cepko, Connie (Public Library of Science, 2005)
      Rod and cone photoreceptors subserve vision under dim and bright light conditions, respectively. The differences in their function are thought to stem from their different gene expression patterns, morphologies, and synaptic ...
    • Identification of Molecular Markers of Bipolar Cells in the Murine Retina 

      Kim, Douglas S; Ross, Sarah Elizabeth; Trimarchi, Jeffrey M; Aach, John Dennis; Greenberg, Michael Eldon; Cepko, Connie (Wiley Subscription Services, Inc., A Wiley Company, 2008)
      Retinal bipolar neurons serve as relay interneurons that connect rod and cone photoreceptor cells to amacrine and ganglion cells. They exhibit diverse morphologies essential for correct routing of photoreceptor cell signals ...
    • Individual Retinal Progenitor Cells Display Extensive Heterogeneity of Gene Expression 

      Trimarchi, Jeffrey M.; Stadler, Michael B.; Cepko, Connie (Public Library of Science, 2008)
      The development of complex tissues requires that mitotic progenitor cells integrate information from the environment. The highly varied outcomes of such integration processes undoubtedly depend at least in part upon ...
    • MicroSAGE is Highly Representative and Reproducible but Reveals Major Differences in Gene Expression Among Samples Obtained from Similar Tissues 

      Blackshaw, Seth; Kuo, Winston Patrick; Park, Peter J.; Tsujikawa, Motokazu; Gunnersen, Jenny M; Scott, Hamish S; Boon, Wee-Ming; Tan, Seong-Seng; Cepko, Constance L. (BioMed Central, 2003)
      Background: Serial analysis of gene expression using small amounts of starting material (microSAGE) has not yet been conclusively shown to be representative, reproducible or accurate. Results: We show that microSAGE is ...
    • Neuroanatomy goes viral! 

      Nassi, Jonathan J.; Cepko, Constance L.; Born, Richard T.; Beier, Kevin T. (Frontiers Media S.A., 2015)
      The nervous system is complex not simply because of the enormous number of neurons it contains but by virtue of the specificity with which they are connected. Unraveling this specificity is the task of neuroanatomy. In ...
    • Postnatal Genome Editing With CRISPR 

      Chew, Wei Leong (2016-05-18)
      Targeted genome editing holds tremendous promise for permanent correction of many genetic diseases. The recently developed CRISPR/Cas9 genome-editing tool exhibits facile programmability and robust gene-editing efficiency, ...
    • Potent Delivery of Functional Proteins into Mammalian Cells in Vitro and in Vivo Using a Supercharged Protein 

      Cronican, James J.; Thompson, David B.; Beier, Kevin T.; McNaughton, Brian R.; Cepko, Connie; Liu, David Ruchien (American Chemical Society (ACS), 2010)
      The inability of proteins to potently penetrate mammalian cells limits their usefulness as tools and therapeutics. When fused to superpositively charged GFP, proteins rapidly (within minutes) entered five different types ...
    • Preferential Budding of Vesicular Stomatitis Virus from the Basolateral Surface of Polarized Epithelial Cells Is Not Solely Directed by Matrix Protein or Glycoprotein 

      Drokhlyansky, Eugene; Soh, Timothy K.; Cepko, Constance L. (American Society for Microbiology, 2015)
      Vesicular stomatitis virus has been shown to bud basolaterally, and the matrix protein, but not glycoprotein, was proposed to mediate this asymmetry. Using polarized T84 monolayers, we demonstrate that no single viral ...
    • RCAS-RNAi: A Loss-of-Function Method for the Developing Chick Retina 

      Harpavat, Sanjiv; Cepko, Connie (BioMed Central, 2006)
      Background: The embryonic chick provides an excellent model system for studies of development. However, it has lacked an efficient loss-of-function method for studies of gene function. Results: We show that avian retroviruses ...
    • Requirement of Histone Deacetylase Activity for the Expression of Critical Photoreceptor Genes 

      Chen, Bo; Cepko, Constance L. (BioMed Central, 2007)
      Background: Histone deacetylases (HDACs) play a major role in the regulation of gene transcription, often leading to transcriptional repression, as well as other effects following deacetylation of non-histone proteins. ...