Now showing items 1-20 of 54

    • Adenosine signaling promotes hematopoietic stem and progenitor cell emergence 

      Jing, Lili; Tamplin, Owen J.; Chen, Michael J.; Deng, Qing; Patterson, Shenia; Kim, Peter G.; Durand, Ellen M.; McNeil, Ashley; Green, Julie M.; Matsuura, Shinobu; Ablain, Julien; Brandt, Margot K.; Schlaeger, Thorsten M.; Huttenlocher, Anna; Daley, George Q.; Ravid, Katya; Zon, Leonard I. (The Rockefeller University Press, 2015)
      Hematopoietic stem cells (HSCs) emerge from aortic endothelium via the endothelial-to-hematopoietic transition (EHT). The molecular mechanisms that initiate and regulate EHT remain poorly understood. Here, we show that ...
    • Angiopoietin-like proteins stimulate HSPC development through interaction with notch receptor signaling 

      Lin, Michelle I; Price, Emily N; Boatman, Sonja; Hagedorn, Elliott J; Trompouki, Eirini; Satishchandran, Sruthi; Carspecken, Charles W; Uong, Audrey; DiBiase, Anthony; Yang, Song; Canver, Matthew C; Dahlberg, Ann; Lu, Zhigang; Zhang, Cheng Cheng; Orkin, Stuart H; Bernstein, Irwin D; Aster, Jon C; White, Richard M; Zon, Leonard I (eLife Sciences Publications, Ltd, 2015)
      Angiopoietin-like proteins (angptls) are capable of ex vivo expansion of mouse and human hematopoietic stem and progenitor cells (HSPCs). Despite this intriguing ability, their mechanism is unknown. In this study, we show ...
    • Caudal Transcription Factors in Hematopoietic Development 

      Paik, Elizabeth Jae-Eun (2013-02-12)
      During embryogenesis, hematopoietic cells arise from the lateral plate mesoderm (LPM) following gastrulation. The transcriptional program required for this LPM to blood switch is not fully understood. Previous work on a ...
    • Ccdc94 Protects Cells from Ionizing Radiation by Inhibiting the Expression of p53 

      Sorrells, Shelly; Carbonneau, Seth; Harrington, Erik; Chen, Aye T.; Hast, Bridgid; Milash, Brett; Major, Michael B.; Stewart, Rodney A.; Jette, Cicely; Pyati, Ujwal Jagdeesh; Zon, Leonard Ira; Look, A. Thomas; Zhou, Yi (Public Library of Science, 2012)
      DNA double-strand breaks (DSBs) represent one of the most deleterious forms of DNA damage to a cell. In cancer therapy, induction of cell death by DNA DSBs by ionizing radiation (IR) and certain chemotherapies is thought ...
    • Cdx4 Is Dispensable for Murine Adult Hematopoietic Stem Cells but Promotes MLL-AF9-Mediated Leukemogenesis 

      Koo, Sumin; Huntly, Brian J.; Wang, Yuan; Chen, Jing; Brumme, Kristina; Ball, Brian; McKinney-Freeman, Shannon L.; Yabuuchi, Akiko; Scholl, Claudia; Bansal, Dimple; Zon, Leonard Ira; Fröhling, Stefan; Daley, George Quentin; Gilliland, D. Gary; Mercher, Thomas (Ferrata Storti Foundation (Haematologica), 2010)
      Background: Cdx4 is a homeobox gene essential for normal blood formation during embryonic development in the zebrafish, through activation of posterior Hox genes. However, its role in adult mammalian hematopoiesis has not ...
    • Chamber identity programs drive early functional partitioning of the heart 

      Mosimann, Christian; Panáková, Daniela; Werdich, Andreas A.; Musso, Gabriel; Burger, Alexa; Lawson, Katy L.; Carr, Logan A.; Nevis, Kathleen R.; Sabeh, M. Khaled; Zhou, Yi; Davidson, Alan J.; DiBiase, Anthony; Burns, Caroline E.; Burns, C. Geoffrey; MacRae, Calum A.; Zon, Leonard I. (Nature Pub. Group, 2015)
      The vertebrate heart muscle (myocardium) develops from the first heart field (FHF) and expands by adding second heart field (SHF) cells. While both lineages exist already in teleosts, the primordial contributions of FHF ...
    • Characterization of immune-matched hematopoietic transplantation in zebrafish 

      de Jong, J. L. O.; Burns, Caroline Erter; Chen, A. T.; Pugach, E.; Mayhall, E. A.; Smith, A. C. H.; Feldman, Henry Arthur; Zhou, Yi; Zon, Leonard Ira (American Society of Hematology, 2011)
      Evaluating hematopoietic stem cell (HSC) function in vivo requires a long-term transplantation assay. Although zebrafish are a powerful model for discovering the genetics of hematopoiesis, hematopoietic transplantation ...
    • A Chemical Genetic Screen in Zebrafish for Pathways Interacting with cdx4 in Primitive Hematopoiesis 

      Paik, Elizabeth Jae-Eun; de Jong, Jill L.O.; Pugach, Emily; Opara, Praise; Zon, Leonard Ira (Mary Ann Liebert Inc, 2010)
      cdx4, a caudal-related homeodomain-containing transcription factor, functions as a regulator of hox genes, thereby playing a critical role in anterior–posterior (A-P) patterning during embryogenesis. In zebrafish, homozygous ...
    • Chemical Genetics of Hematopoietic Stem Cell Transplantation 

      Li, Pulin (2012-09-18)
      Hematopoietic stem and progenitor cells (HSPCs) repopulate the blood system upon transplantation. A large-scale genetic approach to understand the factors that participate in successful engraftment has not been undertaken. ...
    • CHMP1A encodes an essential regulator of BMI1-INK4A in cerebellar development 

      Mochida, Ganeshwaran H.; Ganesh, Vijay S; de Michelena, Maria I.; Dias, Hugo; Atabay, Kutay D.; Kathrein, Katie L.; Huang, Emily; Hill, Robert Sean; Felie, Jillian M.; Rakiec, Daniel; Gleason, Danielle; Hill, Anthony D.; Malik, Athar Naveed; Barry, Brenda J.; Partlow, Jennifer; Tan, Wen-Hann; Glader, Laurie Jean; Barkovich, A. James; Dobyns, William B.; Zon, Leonard Ira; Walsh, Christopher A (2013)
    • Chromatin-Modifying Factors in Zebrafish Models of Rhabdomyosarcoma and Hematopoiesis 

      Albacker, Colleen Elizabeth (2012-12-20)
      Epigenetics, or the reversible and heritable marks of gene regulation not including DNA sequence, encompasses modifications on both the DNA and histones and is as important as the DNA sequence itself. Gene transcription, ...
    • A comparison of non-integrating reprogramming methods 

      Schlaeger, Thorsten M.; Daheron, Laurence Marie; Brickler, Thomas R; Entwisle, Samuel; Chan, Karrie; Cianci, Amelia; DeVine, Alexander; Ettenger, Andrew; Fitzgerald, Kelly; Godfrey, Michelle; Gupta, Dipti; McPherson, Jade; Malwadkar, Prerana; Gupta, Manav; Bell, Blair; Doi, Akiko; Jung, Namyoung; Li, Xin; Lynes, Maureen M.; Brookes, Emily; Cherry, Anne B C; Demirbas, Didem; Tsankov, Alexander; Zon, Leonard Ira; Rubin, Lee; Feinberg, Andrew P; Meissner, Alexander; Cowan, Chad A.; Daley, George Quentin (Nature Publishing Group, 2014)
      Human induced pluripotent stem cells (hiPSCs1, 2, 3) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation ...
    • DHODH modulates transcriptional elongation in the neural crest and melanoma 

      White, Richard Mark; Cech, Jennifer; Ratanasirintrawoot, Sutheera; Lin, Charles P.; Rahl, Peter B.; Burke, Christopher J.; Langdon, Erin; Tomlinson, Matthew L.; Mosher, Jack; Kaufman, Charles Kore; Chen, Frank; Long, Hannah K.; Kramer, Martin; Datta, Sumon Michael; Neuberg, Donna S.; Granter, Scott R.; Young, Richard A.; Morrison, Sean; Wheeler, Grant N.; Zon, Leonard Ira (Nature Publishing Group, 2011)
    • Direct Recruitment of Polycomb Repressive Complex 1 to Chromatin by Core Binding Transcription Factors 

      Yu, Ming; Mazor, Tali; Huang, Hui; Huang, Hsuan-Ting; Kathrein, Katie L.; Woo, Andrew; Chouinard, Candace R.; Labadorf, Adam; Akie, Thomas E.; Moran, Tyler B.; Xie, Huafeng; Zacharek, Sima; Taniuchi, Ichiro; Roeder, Robert G.; Kim, Carla; Zon, Leonard Ira; Fraenkel, Ernest; Cantor, Alan B. (Elsevier BV, 2012)
      Polycomb repressive complexes (PRCs) play key roles in developmental epigenetic regulation. Yet the mechanisms that target PRCs to specific loci in mammalian cells remain incompletely understood. In this study we show that ...
    • DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish 

      Liu, Xiaohui; Jia, Xiaoe; Yuan, Hao; Ma, Ke; Chen, Yi; Jin, Yi; Deng, Min; Pan, Weijun; Chen, Saijuan; Chen, Zhu; de The, Hugues; Zon, Leonard I; Zhou, Yi; Zhou, Jun; Zhu, Jun (BioMed Central, 2015)
      Background: DNA methyltransferase 1 (Dnmt1) regulates expression of many critical genes through maintaining parental DNA methylation patterns on daughter DNA strands during mitosis. It is essential for embryonic development ...
    • Early Cell Fate Determination in Zebrafish 

      Xu, Cong (2013-02-08)
      ESC/iPSC-derive somatic cells may be ideal for treating disorders caused by cellular deficiency or dysfunction. To form a lineage-specific cell population, ESCs/iPSCs undergo a multi-step process that recapitulates embryonic ...
    • Epigenetic Regulation of Hematopoiesis in Zebrafish 

      Huang, Hsuan-Ting (2012-11-02)
      The initiation of the hematopoietic program is orchestrated by key transcription factors that recruit chromatin regulators in order to activate or inhibit blood target gene expression. To generate a complete compendium of ...
    • Epoxyeicosatrienoic Acids Enhance Embryonic Haematopoiesis and Adult Marrow Engraftment 

      Li, Pulin; Lahvic, Jamie L.; Binder, Vera; Pugach, Emily K.; Riley, Elizabeth B.; Tamplin, Owen J.; Panigrahy, Dipak; Bowman, Teresa V.; Barrett, Francesca G.; Heffner, Garrett C.; McKinney-Freeman, Shannon; Schlaeger, Thorsten M.; Daley, George Q.; Zeldin, Darryl C.; Zon, Leonard I. (2015)
      Haematopoietic stem and progenitor cell (HSPC) transplant is a widely used treatment for life-threatening conditions including leukemia; however, the molecular mechanisms regulating HSPC engraftment of the recipient niche ...
    • Extensive Genetic Diversity and Substructuring Among Zebrafish Strains Revealed through Copy Number Variant Analysis 

      Brown, Kim; Dobrinski, Kimberly P.; Lee, Arthur S.; Gokcumen, Omer; Mills, Ryan; Shi, Xinghua; Chong, Wilson W. S.; Chen, Jin Yun Helen; Yoo, Paulo; David, Sthuthi; Peterson, Samuel M.; Raj, Towfique; Choy, Kwong Wai; Stranger, Barbara; Williamson, Robin E.; Zon, Leonard Ira; Freeman, Jennifer L.; Lee, Charles (Proceedings of the National Academy of Sciences, 2012)
      Copy number variants (CNVs) represent a substantial source of genomic variation in vertebrates and have been associated with numerous human diseases. Despite this, the extent of CNVs in the zebrafish, an important model ...
    • Flow-induced protein kinase A–CREB pathway acts via BMP signaling to promote HSC emergence 

      Kim, Peter Geon; Nakano, Haruko; Das, Partha P.; Chen, Michael J.; Rowe, R. Grant; Chou, Stephanie S.; Ross, Samantha J.; Sakamoto, Kathleen M.; Zon, Leonard I.; Schlaeger, Thorsten M.; Orkin, Stuart H.; Nakano, Atsushi; Daley, George Q. (The Rockefeller University Press, 2015)
      Fluid shear stress promotes the emergence of hematopoietic stem cells (HSCs) in the aorta–gonad–mesonephros (AGM) of the developing mouse embryo. We determined that the AGM is enriched for expression of targets of protein ...