Browsing by Author "Harper, Jeffrey"
Now showing items 1-7 of 7
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Deciphering the biological functions of F-box proteins through the use of Parallel Adaptor Capture (PAC) proteomics.
Tan, Meng Kwang Marcus (2013-10-18)The timely and selective proteasomal degradation of proteins is important for the maintenance of proper cellular processes. Prior to proteasomal degradation, proteins destined for degradation are polyubiquitiylated by ... -
Defining the Landscape of the PARKIN- and PINK1-Dependent Ubiquitin-Modified Proteome in Response to Mitochondrial Dysfunction
Sarraf, Shireen Akhavan (2013-09-26)Parkinson's disease (PD) is a progressive neurological disorder resulting from loss of dopaminergic neurons of the substantia nigra, in part due to mitochondrial dysfunction. The E3 ubiquitin ligase, PARKIN, and mitochondrial ... -
A Gain of Function Senescence Bypass Screen Identifies the Homeobox Transcription Factor DLX2 as a Regulator of ATM-P53 Signaling
Wang, Yifan (2016-01-26)Senescence stimuli activate multiple tumor suppressor pathways to initiate cycle arrest and a differentiation program characteristic of senescent cells. We performed a two-stage, gain-of function screen to select for the ... -
A Genetic Screen Identifies FAN1, a Fanconi Anemia-Associated Nuclease Necessary for DNA Interstrand Crosslink Repair
Smogorzewska, Agata; Desetty, Rohini; Saito, Takamune; Schlabach, Michael; Lach, Francis P.; Sowa, Mathew E.; Clark, Alan B.; Kunkel, Thomas A.; Harper, Jeffrey Wade; Colaiacovo, Monica P.; Elledge, Stephen J. (Elsevier BV, 2010)The Fanconi anemia (FA) pathway is responsible for interstrand crosslink repair. At the heart of this pathway is the FANCI-FAND2 (ID) complex, which, upon ubiquitination by the FA core complex, travels to sites of damage ... -
An OBSL1-Cul7\(^{\text{Fbxw8}}\) Ubiquitin Ligase Signaling Mechanism Regulates Golgi Morphology and Dendrite Patterning
Litterman, Nadia Kathryn; Ikeuchi, Yoshiho; Gallardo, Gilbert; O'Connell, Brenda C.; Sowa, Mathew E.; Gygi, Steven P.; Harper, J. Wade; Bonni, Azad (Public Library of Science, 2011)The elaboration of dendrites in neurons requires secretory trafficking through the Golgi apparatus, but the mechanisms that govern Golgi function in neuronal morphogenesis in the brain have remained largely unexplored. ... -
SLX-1 Is Required for Maintaining Genomic Integrity and Promoting Meiotic Noncrossovers in the Caenorhabditis elegans Germline
Saito, Takamune; Mohideen, Firaz; Meyer, Katherine; Harper, J. Wade; Colaiácovo, Monica P. (Public Library of Science, 2012)Although the SLX4 complex, which includes structure-specific nucleases such as XPF, MUS81, and SLX1, plays important roles in the repair of several kinds of DNA damage, the function of SLX1 in the germline remains unknown. ... -
A Systematic Analysis of Factors Localized to Damaged Chromatin Reveals PARP-Dependent Recruitment of Transcription Factors
Izhar, Lior; Adamson, Britt; Ciccia, Alberto; Lewis, Jedd; Pontano-Vaites, Laura; Leng, Yumei; Westbrook, Thomas F.; Elledge, Stephen; Liang, Anthony; Harper, Jeffrey (Elsevier BV, 2015-06)Localization to sites of DNA damage is a hallmark of DNA damage response (DDR) proteins. To identify DDR factors, we screened epitope-tagged proteins for localization to sites of chromatin damaged by UV laser microirradiation ...