Now showing items 1-16 of 16

    • An Active Form of Vav1 Induces Migration of Mammary Epithelial Cells by Stimulating Secretion of an Epidermal Growth Factor Receptor Ligand 

      Wilsbacher, Julie L; Moores, Sheri L; Brugge, Joan S. (BioMed Central, 2006)
      Background: Vav proteins are guanine nucleotide exchange factors (GEF) for Rho family GTPases and are activated following engagement of membrane receptors. Overexpression of Vav proteins enhances lamellipodium and ruffle ...
    • Altered metabolic requirements in cancer cell migration and metastasis 

      Lee, Jaewon; Ng, Mei Rosa; Sinkevicius, Kerstin Wolf; Kim, Carla; Danuser, Gaudenz; Brugge, Joan S.; Haigis, Marcia C. (BioMed Central, 2012)
    • Amplification of phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis 

      Locasale, J.; Grassian, Alexandra; Beroukhim, Rameen; Meyerson, Matthew Langer; Wagner, Gerhard; Asara, John M.; Brugge, Joan S.; Vander Heiden, Matthew George; Cantley, Lewis C. (BioMed Central, 2012)
    • Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours 

      Ince, Tan A.; Sousa, Aurea D.; Jones, Michelle A.; Harrell, J. Chuck; Agoston, Elin S.; Krohn, Marit; Selfors, Laura M.; Liu, Wenbin; Chen, Ken; Yong, Mao; Buchwald, Peter; Wang, Bin; Hale, Katherine S.; Cohick, Evan; Sergent, Petra; Witt, Abigail; Kozhekbaeva, Zhanna; Gao, Sizhen; Agoston, Agoston T.; Merritt, Melissa A.; Foster, Rosemary; Rueda, Bo R.; Crum, Christopher P.; Brugge, Joan S.; Mills, Gordon B. (Nature Pub. Group, 2015)
      Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that ...
    • Evidence for a multipotent mammary progenitor with pregnancy-specific activity 

      Kaanta, Alice S; Virtanen, Carl; Selfors, Laura M; Brugge, Joan S; Neel, Benjamin G (BioMed Central, 2013)
      Introduction: The mouse mammary gland provides a powerful model system for studying processes involved in epithelial tissue development. Although markers that enrich for mammary stem cells and progenitors have been identified, ...
    • Evidence for a Novel Multipotent Mammary Progenitor with Pregnancy-Specific Activity 

      Kaanta, Alice (2012-12-20)
      The mouse mammary gland has emerged as a model system for studying processes involved in the development of epithelial tissues. Current evidence suggests the existence of a differentiation hierarchy in the mammary gland, ...
    • Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters 

      Ng, Mei Rosa; Besser, Achim; Brugge, Joan S; Danuser, Gaudenz (eLife Sciences Publications, Ltd, 2014)
      Force transduction at cell-cell adhesions regulates tissue development, maintenance and adaptation. We developed computational and experimental approaches to quantify, with both sub-cellular and multi-cellular resolution, ...
    • Mechanical Regulation of Epithelial Cell Collective Migration 

      Ng, Mei Rosa (2013-03-05)
      Cell migration is a fundamental biological process involved in tissue development, wound repair, and diseases such as cancer metastasis. It is a biomechanical process involving the adhesion of a cell to a substratum, usually ...
    • Mechanisms Governing Mesothelial Clearance by Ovarian Cancer Spheroids 

      Davidowitz, Rachel Alexis (2013-10-08)
      Metastatic dissemination of ovarian tumors involves the invasion of multi-cellular tumor cell clusters into the mesothelial cell lining of organs in the peritoneal cavity. We developed an in vitro assay that models this ...
    • Molecular Determinants and Transcriptional Regulators in Triple Negative Breast Cancer 

      Subedee, Ashim (2015-08-07)
      Breast cancer is a highly heterogeneous disease with differences in histopathological and biological characteristics, variable prognoses, and response to therapy. Clinically, breast tumors are classified based on the ...
    • Profiling Y561-dependent and -independent substrates of CSF-1R in epithelial cells 

      Knowlton, Melodie L.; Selfors, Laura Marie; Wrobel, Carolyn N.; Gu, Ting-Lei; Ballif, Bryan A.; Gygi, Steven P.; Polakiewicz, Roberto; Brugge, Joan S. (Public Library of Science, 2010)
      Receptor tyrosine kinases (RTKs) activate multiple downstream cytosolic tyrosine kinases following ligand stimulation. SRC family kinases (SFKs), which are recruited to activated RTKs through SH2 domain interactions with ...
    • A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for β-catenin 

      Gallegos, Lisa Leon; Ng, Mei Rosa; Sowa, Mathew E.; Selfors, Laura M.; White, Anne; Zervantonakis, Ioannis K.; Singh, Pragya; Dhakal, Sabin; Harper, J. Wade; Brugge, Joan S. (Nature Publishing Group, 2016)
      Cell-cell adhesion is central to morphogenesis and maintenance of epithelial cell state. We previously identified 27 candidate cell-cell adhesion regulatory proteins (CCARPs) whose down-regulation disrupts epithelial ...
    • PTK6 Regulates IGF-1-Induced Anchorage-Independent Survival 

      Iida, Naoko; Zou, Lihua; Yao, Jun; Lu, Yiling; Epstein, Charles B.; Natesan, Sridaran; Mills, Gordon B.; Irie, Hanna Yoko; Shrestha, Yashaswi; Selfors, Laura Marie; Frye, Fabianne; Wang, Zhigang C.; Richardson, Andrea Lynn; Polyak, Kornelia; Hahn, William C.; Brugge, Joan S. (Public Library of Science, 2010)
      Background: Proteins that are required for anchorage-independent survival of tumor cells represent attractive targets for therapeutic intervention since this property is believed to be critical for survival of tumor cells ...
    • Starved epithelial cells uptake extracellular matrix for survival 

      Muranen, Taru; Iwanicki, Marcin P.; Curry, Natasha L.; Hwang, Julie; DuBois, Cory D.; Coloff, Jonathan L.; Hitchcock, Daniel S.; Clish, Clary B.; Brugge, Joan S.; Kalaany, Nada Y. (Nature Publishing Group, 2017)
      Extracellular matrix adhesion is required for normal epithelial cell survival, nutrient uptake and metabolism. This requirement can be overcome by oncogene activation. Interestingly, inhibition of PI3K/mTOR leads to apoptosis ...
    • Substrate stiffness regulates cadherin-dependent collective migration through myosin-II contractility 

      Ng, Mei Rosa; Besser, Achim; Danuser, Gaudenz; Brugge, Joan S. (The Rockefeller University Press, 2012)
      The mechanical microenvironment is known to influence single-cell migration; however, the extent to which mechanical cues affect collective migration of adherent cells is not well understood. We measured the effects of ...
    • Transcriptional Regulation of Metastatic [Id]entity by KLF17 

      Iwanicki, Marcin; Brugge, Joan S. (BioMed Central, 2009)
      A novel in vivo screening approach has identified KLF17 as a key metastasis suppressor gene that acts through regulation of Id1 transcription factor-dependent induction of the epithelial-to-mesenchymal transition.