Browsing by Author "Cluzel, Philippe"
Now showing items 1-12 of 12
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Adaptive Resistance in Bacteria Requires Epigenetic Inheritance, Genetic Noise, and Cost of Efflux Pumps
Motta, Santiago Sandoval; Cluzel, Philippe; Aldana, Maximino (Public Library of Science, 2015)Adaptive resistance emerges when populations of bacteria are subjected to gradual increases of antibiotics. It is characterized by a rapid emergence of resistance and fast reversibility to the non-resistant phenotype when ... -
Environmental Perturbations Lift the Degeneracy of the Genetic Code to Regulate Protein Levels in Bacteria
Subramaniam, Arvind R; Pan, Tao; Cluzel, Philippe (National Academy of Sciences, 2013)The genetic code underlying protein synthesis is a canonical example of a degenerate biological system. Degeneracies in physical and biological systems can be lifted by external perturbations, thus allowing degenerate ... -
Evidence for the Universal Scaling Behaviour of a Freely Relaxing DNA Molecule
Manneville, S.; Cluzel, Philippe; Viovy, J.-L.; Chatenay, D.; Caron, F. (EDP Sciences, 1996)Relaxation measurements on a fluorescently labelled free DNA molecule after stretching by a Poiseuille flow in a capillary vessel reveal universal scaling features: at intermediate times the scaling exponent of the decay ... -
Fine-Tuning of Chemotactic Response in E. coli Determined by High-Throughput Capillary Assay
Park, Heungwon; Guet, Calin C.; Emonet, Thierry; Cluzel, Philippe (Springer Science + Business Media, 2010)In E. coli, chemotactic behavior exhibits perfect adaptation that is robust to changes in the intracellular concentration of the chemotactic proteins, such as CheR and CheB. However, the robustness of the perfect adaptation ... -
Hidden Stochastic Nature of a Single Bacterial Motor
Korobkova, Ekaterina A.; Emonet, Thierry; Park, Heungwon; Cluzel, Philippe (American Physical Society, 2006)The rotary flagellar motor of Escherichia coli bacterium switches stochastically between the clockwise (CW) and counterclockwise (CCW) direction. We found that the CW and CCW intervals could be described by a gamma ... -
Mechanism-Independent Method for Predicting Response to Multidrug Combinations in Bacteria
Wood, Kevin; Nishida, Satoshi; Sontag, Eduardo D.; Cluzel, Philippe (Proceedings of the National Academy of Sciences, 2012)Drugs are commonly used in combinations larger than two for treating bacterial infection. However, it is generally impossible to infer directly from the effects of individual drugs the net effect of a multidrug combination. ... -
Minimally Invasive Determination of mRNA Concentration in Single Living Bacteria
Guet, Calin C.; Bruneaux, Luke; Min, Taejin L.; Siegal-Gaskins, Dan; Figueroa, Israel; Emonet, Thierry; Cluzel, Philippe (Oxford University Press, 2008)Fluorescence correlation spectroscopy (FCS) has permitted the characterization of high concentrations of noncoding RNAs in a single living bacterium. Here, we extend the use of FCS to low concentrations of coding RNAs in ... -
Noise Underlies Switching Behavior of the Bacterial Flagellum
Park, Heungwon; Oikonomou, Panos; Guet, Calin C.; Cluzel, Philippe (Elsevier BV, 2011)We report the switching behavior of the full bacterial flagellum system that includes the filament and the motor in wild-type Escherichia coli cells. In sorting the motor behavior by the clockwise bias, we find that the ... -
Single-cell behavior
Cluzel, Philippe (CRC Press, 2013) -
The single-cell chemostat: an agarose-based, microfluidic device for high-throughput, single-cell studies of bacteria and bacterial communities
Moffitt, Jeffrey R; Lee, Jeffrey B.; Cluzel, Philippe (Royal Society of Chemistry (RSC), 2012)Optical microscopy of single bacteria growing on solid agarose support is a powerful method for studying the natural heterogeneity in growth and gene expression. While the material properties of agarose make it an excellent ... -
Trade-offs between drug toxicity and benefit in the multi-antibiotic resistance system underlie optimal growth of E. coli
Wood, Kevin B; Cluzel, Philippe (BioMed Central, 2012)Background: Efflux is a widespread mechanism of reversible drug resistance in bacteria that can be triggered by environmental stressors, including many classes of drugs. While such chemicals when used alone are typically ... -
Uncovering Scaling Laws to Infer Multi-drug Response of Resistant Microbes and Cancer Cells
Wood, Kevin B.; Wood, Kris C.; Nishida, Satoshi; Cluzel, Philippe (Elsevier (Cell Press), 2014-02-14)Drug resistance in bacterial infections and cancers constitutes a major threat to human health. Treatments often include several interacting drugs, but even potent therapies can become ineffective in resistant mutants. ...