Now showing items 1-3 of 3

    • Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation 

      Kerenyi, Marc A; Shao, Zhen; Hsu, Yu-Jung; Guo, Guoji; Luc, Sidinh; O'Brien, Kassandra; Fujiwara, Yuko; Peng, Cong; Nguyen, Minh; Orkin, Stuart H (eLife Sciences Publications, Ltd, 2013)
      Here, we describe that lysine-specific demethylase 1 (Lsd1/KDM1a), which demethylates histone H3 on Lys4 or Lys9 (H3K4/K9), is an indispensible epigenetic governor of hematopoietic differentiation. Integrative genomic ...
    • Scl binds to primed enhancers in mesoderm to regulate hematopoietic and cardiac fate divergence 

      Org, Tõnis; Duan, Dan; Ferrari, Roberto; Montel-Hagen, Amelie; Van Handel, Ben; Kerényi, Marc A; Sasidharan, Rajkumar; Rubbi, Liudmilla; Fujiwara, Yuko; Pellegrini, Matteo; Orkin, Stuart H; Kurdistani, Siavash K; Mikkola, Hanna KA (BlackWell Publishing Ltd, 2015)
      Scl/Tal1 confers hemogenic competence and prevents ectopic cardiomyogenesis in embryonic endothelium by unknown mechanisms. We discovered that Scl binds to hematopoietic and cardiac enhancers that become epigenetically ...
    • A Transient Transgenic RNAi Strategy for Rapid Characterization of Gene Function during Embryonic Development 

      Bjork, Bryan Cary; Fujiwara, Yuko; Davis, Shannon W.; Qiu, Haiyan; Saunders, Thomas L.; Sandy, Peter; Orkin, Stuart Holland; Camper, Sally A.; Beier, David Randolph (Public Library of Science, 2010)
      RNA interference (RNAi) is a powerful strategy for studying the phenotypic consequences of reduced gene expression levels in model systems. To develop a method for the rapid characterization of the developmental consequences ...